LOCUS       sequence-127374-         858 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses GFP(1-10)-VP64-NLS in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 858)
  AUTHORS   Kamiyama D, Sekine S, Barsi-Rhyne B, Hu J, Chen B, Gilbert LA, 
            Ishikawa H, Leonetti MD, Marshall WF, Weissman JS, Huang B
  TITLE     Versatile protein tagging in cells with split fluorescent protein.
  JOURNAL   Nat Commun. 2016 Mar 18;7:11046. doi: 10.1038/ncomms11046.
  PUBMED    26988139
REFERENCE   2  (bases 1 to 858)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2016-03-18"; volume: "7:11046. doi"; pages: " 
            10.1038/ncomms11046"
COMMENT     Sequence Label: pHRm-GFP(1-10)-VP64-NLS
FEATURES             Location/Qualifiers
     source          1..858
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             1..642
                     /codon_start=1
                     /product="fragment of superfolder GFP consisting of the 
                     first 10 beta-strands"
                     /label=GFP(1-10)
                     /note="associates with GFP11, the 11th beta-strand, to 
                     reconstitute fluorescent GFP (Cabantous et al., 2005; 
                     Kamiyama et al., 2016)"
                     /translation="MSKGEELFTGVVPILVELDGDVNGHKFSVRGEGEGDATIGKLTLK
                     FICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKRHDFFKSAMPEGYVQERTISFKDDG
                     KYKTRAVVKFEGDTLVNRIELKGTDFKEDGNILGHKLEYNFNSHNVYITADKQKNGIKA
                     NFTVRHNVEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQTVLSKDPNEK"
     CDS             658..807
                     /codon_start=1
                     /product="tetrameric repeat of the minimal activation 
                     domain of herpes simplex virus VP16 (Beerli et al., 1998)"
                     /label=VP64
                     /translation="DALDDFDLDMLGSDALDDFDLDMLGSDALDDFDLDMLGSDALDDF
                     DLDML"
     CDS             835..855
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
ORIGIN
        1 atgtccaaag gagaagaact gtttaccggt gttgtgccaa ttttggttga actcgatggt
       61 gatgtcaacg gacataagtt ctcagtgaga ggcgaaggag aaggtgacgc caccattgga
      121 aaattgactc ttaaattcat ctgtactact ggtaaacttc ctgtaccatg gccgactctc
      181 gtaacaacgc ttacgtacgg agttcagtgc ttttcgagat acccagacca tatgaaaaga
      241 catgactttt ttaagtcggc tatgcctgaa ggttacgtgc aagaaagaac aatttcgttc
      301 aaagatgatg gaaaatataa aactagagca gttgttaaat ttgaaggaga tactttggtt
      361 aaccgcattg aactgaaagg aacagatttt aaagaagatg gtaatattct tggacacaaa
      421 ctcgaataca attttaatag tcataacgta tacatcactg ctgataagca aaagaacgga
      481 attaaagcga atttcacagt acgccataat gtagaagatg gcagtgttca acttgccgac
      541 cattaccaac aaaacacccc tattggagac ggtccggtac ttcttcctga taatcactac
      601 ctctcaacac aaacagtcct gagcaaagat ccaaatgaaa aaggtttaaa cggtagtgat
      661 gctttagacg attttgactt agatatgctt ggttcagacg cgttagacga cttcgaccta
      721 gacatgttag gctcagatgc attggacgac ttcgatttag atatgttggg ctccgatgcc
      781 ctagatgact ttgatctaga tatgctaggt agtactagtg gtggcggcgg aagtcccaag
      841 aagaagcgca aggtgtaa
//
