LOCUS       sequence-127377-        9943 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  MLV retroviral vector expressing the hTERT reverse transcriptase 
            immortalizing gene and a GFP marker.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9943)
  TITLE     xlox(GFP)hTERT
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 9943)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: xlox(GFP)hTERT
FEATURES             Location/Qualifiers
     source          1..9943
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    580..921
                     /label=MESV Psi
                     /note="packaging signal of murine embryonic stem cell 
                     virus"
     CDS             988..1404
                     /label=gag (truncated)
                     /note="truncated Moloney murine leukemia virus (MMLV) gag 
                     gene lacking the start codon"
     primer_bind     1334..1356
                     /label=pLXSN 5'
                     /note="Murine stem cell virus, forward primer. Also called 
                     MSCV"
     primer_bind     1372..1388
                     /label=pBABE 5'
                     /note="Psi packaging signal, forward primer for pBABE 
                     vectors"
     CDS             1470..4868
                     /codon_start=1
                     /gene="TERT"
                     /product="human telomerase reverse transcriptase, the 
                     catalytic subunit of telomerase"
                     /label=hTERT
                     /note="prevents telomere shortening and allows 
                     postsenescent cells to proliferate beyond crisis (Counter 
                     et al., 1998); also known as EST2"
                     /translation="MPRAPRCRAVRSLLRSHYREVLPLATFVRRLGPQGWRLVQRGDPA
                     AFRALVAQCLVCVPWDARPPPAAPSFRQVSCLKELVARVLQRLCERGAKNVLAFGFALL
                     DGARGGPPEAFTTSVRSYLPNTVTDALRGSGAWGLLLRRVGDDVLVHLLARCALFVLVA
                     PSCAYQVCGPPLYQLGAATQARPPPHASGPRRRLGCERAWNHSVREAGVPLGLPAPGAR
                     RRGGSASRSLPLPKRPRRGAAPEPERTPVGQGSWAHPGRTRGPSDRGFCVVSPARPAEE
                     ATSLEGALSGTRHSHPSVGRQHHAGPPSTSRPPRPWDTPCPPVYAETKHFLYSSGDKEQ
                     LRPSFLLSSLRPSLTGARRLVETIFLGSRPWMPGTPRRLPRLPQRYWQMRPLFLELLGN
                     HAQCPYGVLLKTHCPLRAAVTPAAGVCAREKPQGSVAAPEEEDTDPRRLVQLLRQHSSP
                     WQVYGFVRACLRRLVPPGLWGSRHNERRFLRNTKKFISLGKHAKLSLQELTWKMSVRGC
                     AWLRRSPGVGCVPAAEHRLREEILAKFLHWLMSVYVVELLRSFFYVTETTFQKNRLFFY
                     RKSVWSKLQSIGIRQHLKRVQLRELSEAEVRQHREARPALLTSRLRFIPKPDGLRPIVN
                     MDYVVGARTFRREKRAERLTSRVKALFSVLNYERARRPGLLGASVLGLDDIHRAWRTFV
                     LRVRAQDPPPELYFVKVDVTGAYDTIPQDRLTEVIASIIKPQNTYCVRRYAVVQKAAHG
                     HVRKAFKSHVSTLTDLQPYMRQFVAHLQETSPLRDAVVIEQSSSLNEASSGLFDVFLRF
                     MCHHAVRIRGKSYVQCQGIPQGSILSTLLCSLCYGDMENKLFAGIRRDGLLLRLVDDFL
                     LVTPHLTHAKTFLRTLVRGVPEYGCVVNLRKTVVNFPVEDEALGGTAFVQMPAHGLFPW
                     CGLLLDTRTLEVQSDYSSYARTSIRASLTFNRGFKAGRNMRRKLFGVLRLKCHSLFLDL
                     QVNSLQTVCTNIYKILLLQAYRFHACVLQLPFHQQVWKNPTFFLRVISDTASLCYSILK
                     AKNAGMSLGAKGAAGPLPSEAVQWLCHQAFLLKLTRHRVTYVPLLGSLRTAQTQLSRKL
                     PGTTLTALEAAANPALPSDFKTILD"
     primer_bind     complement(4947..4970)
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     primer_bind     5336..5355
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     CDS             join(5423..5425,5426..5428,5429..6142)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(5423..5425,5426..5428,5429..6139)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(5468..5489)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     6076..6097
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     LTR             6218..6733
                     /label=3' LTR
                     /note="3' long terminal repeat from murine embryonic stem 
                     cell virus"
     primer_bind     complement(6902..6918)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6902..6918)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(6915..6937)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    6926..6942
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(6950..6956,6957..6974,6975..6980))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6995..7016
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(7133..7150)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(7304..7892)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7384..7403)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(8063..8854,8855..8923))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     8686..8705
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(8924..9028)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     9096..9114
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(9152..9174)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     9274..9293
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     9487..9509
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     9501..9518
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     9502..9518
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     9721..9740
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     LTR             join(9942..9943,1..516)
                     /label=5' LTR
                     /note="5' long terminal repeat from murine embryonic stem 
                     cell virus"
ORIGIN
        1 tgaaagaccc cacctgtagg tttggcaagc tagcttaagt aacgccattt tgcaaggcat
       61 ggaaaataca taactgagaa tagagaagtt cagatcaagg ttaggaacag agagacagca
      121 gaatatgggc caaacaggat atctgtggta agcagttcct gccccggctc agggccaaga
      181 acagatggtc cccagatgcg gtcccgccct cagcagtttc tagagaacca tcagatgttt
      241 ccagggtgcc ccaaggacct gaaaatgacc ctgtgcctta tttgaactaa ccaatcagtt
      301 cgcttctcgc ttctgttcgc gcgcttctgc tccccgagct caataaaaga gcccacaacc
      361 cctcactcgg cgcgccagtc ctccgataga ctgcgtcgcc cgggtacccg tattcccaat
      421 aaagcctctt gctgtttgca tccgaatcgt ggactcgctg atccttggga gggtctcctc
      481 agattgattg actgcccacc tcgggggtct ttcatttgga ggttccaccg agatttggag
      541 acccctgcct agggaccacc gacccccccg ccgggaggta agctggccag cggtcgtttc
      601 gtgtctgtct ctgtctttgt gcgtgtttgt gccggcatct aatgtttgcg cctgcgtctg
      661 tactagttag ctaactagct ctgtatctgg cggacccgtg gtggaactga cgagttctga
      721 acacccggcc gcaaccctgg gagacgtccc agggactttg ggggccgttt ttgtggcccg
      781 acctgaggaa gggagtcgat gtggaatccg accccgtcag gatatgtggt tctggtagga
      841 gacgagaacc taaaacagtt cccgcctccg tctgaatttt tgctttcggt ttggaaccga
      901 agccgcgcgt cttgtctgct gcagcgctgc agcatcgttc tgtgttgtct ctgtctgact
      961 gtgtttctgt atttgtctga aaattagggc cagactgtta ccactccctt aagtttgacc
     1021 ttaggtcact ggaaagatgt cgagcggatc gctcacaacc agtcggtaga tgtcaagaag
     1081 agacgttggg ttaccttctg ctctgcagaa tggccaacct ttaacgtcgg atggccgcga
     1141 gacggcacct ttaaccgaga cctcatcacc caggttaaga tcaaggtctt ttcacctggc
     1201 ccgcatggac acccagacca ggtcccctac atcgtgacct gggaagcctt ggcttttgac
     1261 ccccctccct gggtcaagcc ctttgtacac cctaagcctc cgcctcctct tcctccatcc
     1321 gccccgtctc tcccccttga acctcctcgt tcgaccccgc ctcgatcctc cctttatcca
     1381 gccctcactc cttctctagg cgccggaatt agatcccgga attcgtcctg ctgcgcacgt
     1441 gggaagccct ggccccggcc acccccgcga tgccgcgcgc tccccgctgc cgagccgtgc
     1501 gctccctgct gcgcagccac taccgcgagg tgctgccgct ggccacgttc gtgcggcgcc
     1561 tggggcccca gggctggcgg ctggtgcagc gcggggaccc ggcggctttc cgcgcgctgg
     1621 tggcccagtg cctggtgtgc gtgccctggg acgcacggcc gccccccgcc gccccctcct
     1681 tccgccaggt gtcctgcctg aaggagctgg tggcccgagt gctgcagagg ctgtgcgagc
     1741 gcggcgcgaa gaacgtgctg gccttcggct tcgcgctgct ggacggggcc cgcgggggcc
     1801 cccccgaggc cttcaccacc agcgtgcgca gctacctgcc caacacggtg accgacgcac
     1861 tgcgggggag cggggcgtgg gggctgctgc tgcgccgcgt gggcgacgac gtgctggttc
     1921 acctgctggc acgctgcgcg ctctttgtgc tggtggctcc cagctgcgcc taccaggtgt
     1981 gcgggccgcc gctgtaccag ctcggcgctg ccactcaggc ccggcccccg ccacacgcta
     2041 gtggaccccg aaggcgtctg ggatgcgaac gggcctggaa ccatagcgtc agggaggccg
     2101 gggtccccct gggcctgcca gccccgggtg cgaggaggcg cgggggcagt gccagccgaa
     2161 gtctgccgtt gcccaagagg cccaggcgtg gcgctgcccc tgagccggag cggacgcccg
     2221 ttgggcaggg gtcctgggcc cacccgggca ggacgcgtgg accgagtgac cgtggtttct
     2281 gtgtggtgtc acctgccaga cccgccgaag aagccacctc tttggagggt gcgctctctg
     2341 gcacgcgcca ctcccaccca tccgtgggcc gccagcacca cgcgggcccc ccatccacat
     2401 cgcggccacc acgtccctgg gacacgcctt gtcccccggt gtacgccgag accaagcact
     2461 tcctctactc ctcaggcgac aaggagcagc tgcggccctc cttcctactc agctctctga
     2521 ggcccagcct gactggcgct cggaggctcg tggagaccat ctttctgggt tccaggccct
     2581 ggatgccagg gactccccgc aggttgcccc gcctgcccca gcgctactgg caaatgcggc
     2641 ccctgtttct ggagctgctt gggaaccacg cgcagtgccc ctacggggtg ctcctcaaga
     2701 cgcactgccc gctgcgagct gcggtcaccc cagcagccgg tgtctgtgcc cgggagaagc
     2761 cccagggctc tgtggcggcc cccgaggagg aggacacaga cccccgtcgc ctggtgcagc
     2821 tgctccgcca gcacagcagc ccctggcagg tgtacggctt cgtgcgggcc tgcctgcgcc
     2881 ggctggtgcc cccaggcctc tggggctcca ggcacaacga acgccgcttc ctcaggaaca
     2941 ccaagaagtt catctccctg gggaagcatg ccaagctctc gctgcaggag ctgacgtgga
     3001 agatgagcgt gcggggctgc gcttggctgc gcaggagccc aggggttggc tgtgttccgg
     3061 ccgcagagca ccgtctgcgt gaggagatcc tggccaagtt cctgcactgg ctgatgagtg
     3121 tgtacgtcgt cgagctgctc aggtctttct tttatgtcac ggagaccacg tttcaaaaga
     3181 acaggctctt tttctaccgg aagagtgtct ggagcaagtt gcaaagcatt ggaatcagac
     3241 agcacttgaa gagggtgcag ctgcgggagc tgtcggaagc agaggtcagg cagcatcggg
     3301 aagccaggcc cgccctgctg acgtccagac tccgcttcat ccccaagcct gacgggctgc
     3361 ggccgattgt gaacatggac tacgtcgtgg gagccagaac gttccgcaga gaaaagaggg
     3421 ccgagcgtct cacctcgagg gtgaaggcac tgttcagcgt gctcaactac gagcgggcgc
     3481 ggcgccccgg cctcctgggc gcctctgtgc tgggcctgga cgatatccac agggcctggc
     3541 gcaccttcgt gctgcgtgtg cgggcccagg acccgccgcc tgagctgtac tttgtcaagg
     3601 tggatgtgac gggcgcgtac gacaccatcc cccaggacag gctcacggag gtcatcgcca
     3661 gcatcatcaa accccagaac acgtactgcg tgcgtcggta tgccgtggtc cagaaggccg
     3721 cccatgggca cgtccgcaag gccttcaaga gccacgtctc taccttgaca gacctccagc
     3781 cgtacatgcg acagttcgtg gctcacctgc aggagaccag cccgctgagg gatgccgtcg
     3841 tcatcgagca gagctcctcc ctgaatgagg ccagcagtgg cctcttcgac gtcttcctac
     3901 gcttcatgtg ccaccacgcc gtgcgcatca ggggcaagtc ctacgtccag tgccagggga
     3961 tcccgcaggg ctccatcctc tccacgctgc tctgcagcct gtgctacggc gacatggaga
     4021 acaagctgtt tgcggggatt cggcgggacg ggctgctcct gcgtttggtg gatgatttct
     4081 tgttggtgac acctcacctc acccacgcga aaaccttcct caggaccctg gtccgaggtg
     4141 tccctgagta tggctgcgtg gtgaacttgc ggaagacagt ggtgaacttc cctgtagaag
     4201 acgaggccct gggtggcacg gcttttgttc agatgccggc ccacggccta ttcccctggt
     4261 gcggcctgct gctggatacc cggaccctgg aggtgcagag cgactactcc agctatgccc
     4321 ggacctccat cagagccagt ctcaccttca accgcggctt caaggctggg aggaacatgc
     4381 gtcgcaaact ctttggggtc ttgcggctga agtgtcacag cctgtttctg gatttgcagg
     4441 tgaacagcct ccagacggtg tgcaccaaca tctacaagat cctcctgctg caggcgtaca
     4501 ggtttcacgc atgtgtgctg cagctcccat ttcatcagca agtttggaag aaccccacat
     4561 ttttcctgcg cgtcatctct gacacggcct ccctctgcta ctccatcctg aaagccaaga
     4621 acgcagggat gtcgctgggg gccaagggcg ccgccggccc tctgccctcc gaggccgtgc
     4681 agtggctgtg ccaccaagca ttcctgctca agctgactcg acaccgtgtc acctacgtgc
     4741 cactcctggg gtcactcagg acagcccaga cgcagctgag tcggaagctc ccggggacga
     4801 cgctgactgc cctggaggcc gcagccaacc cggcactgcc ctcagacttc aagaccatcc
     4861 tggactgagt cgacccgggc ggccccggaa ttccgggatc tctcgagtac cgggtagggg
     4921 aggcgctttt cccaaggcag tctggagcat gcgctttagc agccccgctg gcacttggcg
     4981 ctacacaagt ggcctctggc ctcgcacaca ttccacatcc accggtagcg ccaaccggct
     5041 ccgttctttg gtggcccctt cgcgccactt ctactcctcc cctagtcagg aagtttcccc
     5101 cagcaagctc gcgtcgtgca ggacgtgaca aatggaagta gcacgtctca ctagtctcgt
     5161 gcagatggac agcaccgctg agcaatggaa gcgggtaggc ctttggggca gcggccaata
     5221 gcagctttgt tccttcgctt tctgggctca gaggctggga aggggtgggt ccgggggcgg
     5281 gctcaggggc gggctcaggg gcgggcgggc gcccgaaggt cctcccgagg cccggcattc
     5341 tgcacgcttc aaaagcgcac gtctgccgcg ctgttctcct cttcctcatc tccgggcctt
     5401 tcgacctgca ggcggccgca ccatggtgag caagggcgag gagctgttca ccggggtggt
     5461 gcccatcctg gtcgagctgg acggcgacgt aaacggccac aagttcagcg tgtccggcga
     5521 gggcgagggc gatgccacct acggcaagct gaccctgaag ttcatctgca ccaccggcaa
     5581 gctgcccgtg ccctggccca ccctcgtgac caccctgacc tacggcgtgc agtgcttcag
     5641 ccgctacccc gaccacatga agcagcacga cttcttcaag tccgccatgc ccgaaggcta
     5701 cgtccaggag cgcaccatct tcttcaagga cgacggcaac tacaagaccc gcgccgaggt
     5761 gaagttcgag ggcgacaccc tggtgaaccg catcgagctg aagggcatcg acttcaagga
     5821 ggacggcaac atcctggggc acaagctgga gtacaactac aacagccaca acgtctatat
     5881 catggccgac aagcagaaga acggcatcaa ggtgaacttc aagatccgcc acaacatcga
     5941 ggacggcagc gtgcagctcg ccgaccacta ccagcagaac acccccatcg gcgacggccc
     6001 cgtgctgctg cccgacaacc actacctgag cacccagtcc gccctgagca aagaccccaa
     6061 cgagaagcgc gatcacatgg tcctgctgga gttcgtgacc gccgccggga tcactctcgg
     6121 catggacgag ctgtacaagt aagcggccgc aatcgacctg cagccaagct tatcgataaa
     6181 ataaaagatt ttatttagtc tccagaaaaa ggggggaatg aaagacccca cctgtaggtt
     6241 tggcaagcta gcttaagtaa cgccattttg caaggcatgg aaaatacata actgagaata
     6301 gagaagttca gatcaaggtt aggaacagag agacagcaga atatgggcca aacaggatat
     6361 ctgtggtaag cagttcctgc cccggctcag ggccaagaac agatggtccc cagatgcggt
     6421 cccgccctca gcagtttcta gagaaccatc agatgtttcc agggtgcccc aaggacctga
     6481 aaatgaccct gtgccttatt tgaactaacc aatcagttcg cttctcgctt ctgttcgcgc
     6541 gcttctgctc cccgagctca ataaaagagc ccacaacccc tcactcggcg cgccagtcct
     6601 ccgatagact gcgtcgcccg ggtacccgtg tatccaataa accctcttgc agttgcatcc
     6661 gacttgtggt ctcgctgttc cttgggaggg tctcctctga gtgattgact acccgtcagc
     6721 gggggtcttt catgggtaac agtttcttga agttggagaa caacattctg agggtaggag
     6781 tcgaatatta agtaatcctg actcaattag ccactgtttt gaatccacat actccaatac
     6841 tcctgaaata gttcattatg gacagcgcag aagagctggg gagaattaat tcgtaatcat
     6901 ggtcatagct gtttcctgtg tgaaattgtt atccgctcac aattccacac aacatacgag
     6961 ccggaagcat aaagtgtaaa gcctggggtg cctaatgagt gagctaactc acattaattg
     7021 cgttgcgctc actgcccgct ttccagtcgg gaaacctgtc gtgccagctg cattaatgaa
     7081 tcggccaacg cgcggggaga ggcggtttgc gtattgggcg ctcttccgct tcctcgctca
     7141 ctgactcgct gcgctcggtc gttcggctgc ggcgagcggt atcagctcac tcaaaggcgg
     7201 taatacggtt atccacagaa tcaggggata acgcaggaaa gaacatgtga gcaaaaggcc
     7261 agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc gtttttccat aggctccgcc
     7321 cccctgacga gcatcacaaa aatcgacgct caagtcagag gtggcgaaac ccgacaggac
     7381 tataaagata ccaggcgttt ccccctggaa gctccctcgt gcgctctcct gttccgaccc
     7441 tgccgcttac cggatacctg tccgcctttc tcccttcggg aagcgtggcg ctttctcata
     7501 gctcacgctg taggtatctc agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc
     7561 acgaaccccc cgttcagccc gaccgctgcg ccttatccgg taactatcgt cttgagtcca
     7621 acccggtaag acacgactta tcgccactgg cagcagccac tggtaacagg attagcagag
     7681 cgaggtatgt aggcggtgct acagagttct tgaagtggtg gcctaactac ggctacacta
     7741 gaaggacagt atttggtatc tgcgctctgc tgaagccagt taccttcgga aaaagagttg
     7801 gtagctcttg atccggcaaa caaaccaccg ctggtagcgg tggttttttt gtttgcaagc
     7861 agcagattac gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt tctacggggt
     7921 ctgacgctca gtggaacgaa aactcacgtt aagggatttt ggtcatgaga ttatcaaaaa
     7981 ggatcttcac ctagatcctt ttaaattaaa aatgaagttt taaatcaatc taaagtatat
     8041 atgagtaaac ttggtctgac agttaccaat gcttaatcag tgaggcacct atctcagcga
     8101 tctgtctatt tcgttcatcc atagttgcct gactccccgt cgtgtagata actacgatac
     8161 gggagggctt accatctggc cccagtgctg caatgatacc gcgagaccca cgctcaccgg
     8221 ctccagattt atcagcaata aaccagccag ccggaagggc cgagcgcaga agtggtcctg
     8281 caactttatc cgcctccatc cagtctatta attgttgccg ggaagctaga gtaagtagtt
     8341 cgccagttaa tagtttgcgc aacgttgttg ccattgctac aggcatcgtg gtgtcacgct
     8401 cgtcgtttgg tatggcttca ttcagctccg gttcccaacg atcaaggcga gttacatgat
     8461 cccccatgtt gtgcaaaaaa gcggttagct ccttcggtcc tccgatcgtt gtcagaagta
     8521 agttggccgc agtgttatca ctcatggtta tggcagcact gcataattct cttactgtca
     8581 tgccatccgt aagatgcttt tctgtgactg gtgagtactc aaccaagtca ttctgagaat
     8641 agtgtatgcg gcgaccgagt tgctcttgcc cggcgtcaat acgggataat accgcgccac
     8701 atagcagaac tttaaaagtg ctcatcattg gaaaacgttc ttcggggcga aaactctcaa
     8761 ggatcttacc gctgttgaga tccagttcga tgtaacccac tcgtgcaccc aactgatctt
     8821 cagcatcttt tactttcacc agcgtttctg ggtgagcaaa aacaggaagg caaaatgccg
     8881 caaaaaaggg aataagggcg acacggaaat gttgaatact catactcttc ctttttcaat
     8941 attattgaag catttatcag ggttattgtc tcatgagcgg atacatattt gaatgtattt
     9001 agaaaaataa acaaataggg gttccgcgca catttccccg aaaagtgcca cctgacgtct
     9061 aagaaaccat tattatcatg acattaacct ataaaaatag gcgtatcacg aggccctttc
     9121 gtctcgcgcg tttcggtgat gacggtgaaa acctctgaca catgcagctc ccggagacgg
     9181 tcacagcttg tctgtaagcg gatgccggga gcagacaagc ccgtcagggc gcgtcagcgg
     9241 gtgttggcgg gtgtcggggc tggcttaact atgcggcatc agagcagatt gtactgagag
     9301 tgcaccatat gcggtgtgaa ataccgcaca gatgcgtaag gagaaaatac cgcatcaggc
     9361 gccattcgcc attcaggctg cgcaactgtt gggaagggcg atcggtgcgg gcctcttcgc
     9421 tattacgcca gctggcgaaa gggggatgtg ctgcaaggcg attaagttgg gtaacgccag
     9481 ggttttccca gtcacgacgt tgtaaaacga cggccagtgc caagctcgct ctcccttatg
     9541 cgactcctgc attaggaagc agcccagtag taggttgagg ccgttgagca ccgccgccgc
     9601 aaggaatggt gcatgcaagg agatggcgcc caacagtccc ccggccacgg ggcctgccac
     9661 catacccacg ccgaaacaag cgctcatgag cccgaagtgg cgagcccgat cttccccatc
     9721 ggtgatgtcg gcgatatagg cgccagcaac cgcacctgtg gcgccggtga tgccggccac
     9781 gatgcgtccg gcgtagagga tcgattagtc caatttgtta aagacaggat atcagtggtc
     9841 caggctctag ttttgactca acaatatcac cagctgaagc ctatagagta cgagccatag
     9901 ataaaataaa agattttatt tagtctccag aaaaaggggg gaa
//
