LOCUS       sequence-127379-        1055 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  mammalian expression of human AICD fused to EGFP.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1055)
  AUTHORS   Currinn H, Guscott B, Balklava Z, Rothnie A, Wassmer T
  TITLE     APP controls the formation of PI(3,5)P vesicles through its binding 
            of the PIKfyve complex.
  JOURNAL   Cell Mol Life Sci. 2015 Jul 28.
  PUBMED    26216398
REFERENCE   2  (bases 1 to 1055)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell Mol 
            Life Sci. 2015 Jul 28."
COMMENT     Sequence Label: pEGFP-n1-AICD
FEATURES             Location/Qualifiers
     source          1..1055
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     213..234
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     regulatory      276..285
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     promoter        complement(494..697)
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     complement(523..543)
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     enhancer        698..1001
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
ORIGIN
        1 gtagccttcg ggcatggcgg acttgaagaa gtcgtgctgc ttcatgtggt cggggtagcg
       61 gctgaagcac tgcacgccgt aggtcagggt ggtcacgagg gtgggccagg gcacgggcag
      121 cttgccggtg gtgcagatga acttcagggt cagcttgccg taggtggcat cgccctcgcc
      181 ctcgccggac acgctgaact tgtggccgtt tacgtcgccg tccagctcga ccaggatggg
      241 caccaccccg gtgaacagct cctcgccctt gctcaccatg gtggcgaccg gtggatcccg
      301 ggcccgcggt accgtcgacc cgttctgcat ctgctcaaag aacttgtagg ttggattttc
      361 gtagccgttc tgctgcatct tggacaggtg gcgctcctct ggggtgacag cggcgtcaac
      421 ctccaccaca ccatgatgaa tggatgtgta ctgtttcttc ttcatgctag cggatctgac
      481 ggttcactaa accagctctg cttatataga cctcccaccg tacacgccta ccgcccattt
      541 gcgtcaatgg ggcggagttg ttacgacatt ttggaaagtc ccgttgattt tggtgccaaa
      601 acaaactccc attgacgtca atggggtgga gacttggaaa tccccgtgag tcaaaccgct
      661 atccacgccc attgatgtac tgccaaaacc gcatcaccat ggtaatagcg atgactaata
      721 cgtagatgta ctgccaagta ggaaagtccc ataaggtcat gtactgggca taatgccagg
      781 cgggccattt accgtcattg acgtcaatag ggggcgtact tggcatatga tacacttgat
      841 gtactgccaa gtgggcagtt taccgtaaat actccaccca ttgacgtcaa tggaaagtcc
      901 ctattggcgt tactatggga acatacgtca ttattgacgt caatgggcgg gggtcgttgg
      961 gcggtcagcc aggcgggcca tttaccgtaa gttatgtaac gcggaactcc atatatgggc
     1021 tatgaactaa tgaccccgta attgattact attaa
//
