LOCUS       sequence-127388-        1078 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  5' Entry vector containing the zebrafish mfap4 promoter. Used with 
            Gateway Recombination to generate transgenic constructs exhibiting 
            macrophage-restricted expression of transgenes..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1078)
  AUTHORS   Walton EM, Cronan MR, Beerman RW, Tobin DM
  TITLE     The Macrophage-Specific Promoter mfap4 Allows Live, Long-Term 
            Analysis of Macrophage Behavior during Mycobacterial Infection in 
            Zebrafish.
  JOURNAL   PLoS One. 2015 Oct 7;10(10):e0138949. doi: 
            10.1371/journal.pone.0138949. eCollection 2015.
  PUBMED    26445458
REFERENCE   2  (bases 1 to 1078)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2015-10-7"; volume: "10(10):e0138949. doi"; pages: " 
            10.1371/journal.pone.0138949. eCollection 2015"
COMMENT     Sequence Label: p5E-mfap4
FEATURES             Location/Qualifiers
     source          1..1078
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    22..117
                     /gene="mutant version of attL"
                     /label=attL4
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     119..138
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        119..137
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
ORIGIN
        1 ttaacgctac catggagctc caaataatga ttttattttg actgatagtg acctgttcgt
       61 tgcaacaaat tgataagcaa tgctttttta taatgccaac tttgtataga aaagttggta
      121 atacgactca ctatagggcg aattgggtac cgggcccccc ctcgaggcgt ttcttggtac
      181 agctggaaag ggaataacgc ggtcagtatg aaaaccatca gcatcaagat caaacaagtg
      241 ccttagaaac agggctttta aaaggaaacc tcttctcttt taaatgtgta tctgaaaaat
      301 cgagtctgaa atgtatggct gttggtgtaa tgacattttt ctctttgttt tcactttaaa
      361 gctttaataa actcctgtat gcagatttat gagattgtgt cctgctattc tttgaaacaa
      421 aagctcattt tccattcaaa taatgggaac tggattaatg tacaaaactg taaaagggca
      481 taaagcattt gtggataaag cagagtttcc atctaatgag acaaagacaa cacacaataa
      541 atgcaacgct gtggcttttg aaggtgagag atgctctttg ggatgctcaa gacagttctg
      601 gaggtaataa taataataat aatactgaac cactgtgatg acagacttta aggcatttta
      661 caataagcaa aacaacattt cagatgtttt acagtgtgct ggaattaaat gctgcaaagt
      721 ccattgatgc gtcccatcac gcacactttt ttctgatcat catctgttca aataaatcac
      781 attacatatg ctgcagcttt ctggattgtc tgtagaggtt tatgggtgca aggcaggagt
      841 ccagctagaa gagcattgca atagtccagc tttttggact aaatgttgtg cagtatgatc
      901 tcttaagaag ggtctgatgt taaagagtgc aaacaatttt tcactgaggg attcacacat
      961 gcagatatgt ctgataatca gatcatccat ataaattaga aatattaaga aatcgagtgg
     1021 atttacagcc agattcttag tgttcgctct taacttgcgc catttgatta gctctgtc
//
