LOCUS       sequence-127389-         992 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  5' Entry vector containing the zebrafish mfap4 promoter. Used with 
            Gateway Recombination to generate transgenic constructs exhibiting 
            macrophage-restricted expression of transgenes..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 992)
  AUTHORS   Walton EM, Cronan MR, Beerman RW, Tobin DM
  TITLE     The Macrophage-Specific Promoter mfap4 Allows Live, Long-Term 
            Analysis of Macrophage Behavior during Mycobacterial Infection in 
            Zebrafish.
  JOURNAL   PLoS One. 2015 Oct 7;10(10):e0138949. doi: 
            10.1371/journal.pone.0138949. eCollection 2015.
  PUBMED    26445458
REFERENCE   2  (bases 1 to 992)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2015-10-7"; volume: "10(10):e0138949. doi"; pages: " 
            10.1371/journal.pone.0138949. eCollection 2015"
COMMENT     Sequence Label: p5E-mfap4
FEATURES             Location/Qualifiers
     source          1..992
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    complement(80..203)
                     /gene="mutant version of attR"
                     /label=attR1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     promoter        204..222
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     259..275
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     259..275
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
ORIGIN
        1 ctatagggga tatcgcggcc gccctgcagc tctagagctc gaattctaca ggtcactaat
       61 accatctaag tagttggttc atagtgactg catatgttgt gttttacagt attatgtagt
      121 ctgtttttta tgcaaaatct aatttaatat attgatattt atatcatttt acgtttctcg
      181 ttcaactttt ttgtacaaac ttgaattaac cctcactaaa gggaacaaaa gctggagctc
      241 caccgcggtg gcggccgctc tagaactagt ggatcccacg atctaaagtc atgaagaaag
      301 aaatcaccaa tacgagacaa aatggcgtgt gtaaatgtga tcgtcacaag gaataaaaca
      361 gccacagaac ttactgcaat cttctcactc tctcctcaac agctcagttt atctacaaga
      421 gatgtaaaag agcttcactc acaccttctt attgttgcag gttatttctt cgtataatct
      481 cgctctacag aataagtcac tcaccgtgtg tgttcagctg gaaataactc aaacgctcct
      541 ttaaatcgct gatgctgagt ctcgtgctct gtgacaaaac ctgcttgagg caatcctcaa
      601 aaacccaaca atctgttttc acaaccgaag agtgaaagga cagcagttct cggttctgtt
      661 ttctgccaaa tctgcaaacg cagacaaatg ttatgataac cccaccaaat aacccttttc
      721 tgcagaagat aacaaacaca cgattcaacc tgcacaacac taatgacttt tttcaaaaca
      781 actataaaac attggttggt taaaagcttg gttgaggagt aaaaggacac aatgcagaga
      841 taagtgcaat gttaacctgg agtctgcatt tacaagactc taaattctct aacacaagaa
      901 ccagtcgagt gttctctttg ggttaacatt gactgattca gcatacacaa gaatacctga
      961 agtgacagag ctaatcaaat ggcgcaagtt aa
//
