LOCUS       sequence-127392-         812 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  bacterial expression of G2K (d-glycerate 2-kinase).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 812)
  AUTHORS   Sims, P, Reed, G
  TITLE     Method for the enzymatic synthesis of 2-phospho-d-glycerate from 
            adenosine 5′-triphosphate and d-glycerate via d-glycerate-2-kinase
  JOURNAL   Journal of Molecular Catalysis B: Enzymatic 32 (2005) 77–81
REFERENCE   2  (bases 1 to 812)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Journal of 
            Molecular Catalysis B: Enzymatic 32 (2005) 77–81"
COMMENT     Sequence Label: pET-G2K
FEATURES             Location/Qualifiers
     source          1..812
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(39..56)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             complement(66..107)
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
ORIGIN
        1 cttcctttcg ggctttgtta gcagccggat caaactcaat ggtgatggtg atgatgaccg
       61 gtacgcgtag aatcgagacc gaggagaggg ttagggatag gcttaccttc aagcttcgaa
      121 ttgagctcgc ccttcgcgtt gcgcattcca atcgccagtg tcgcggcgat attacgtgaa
      181 gcacggcaga tattgtcata agccccgcgg aatgcttcgt ccaacgtacc tatgctggtc
      241 aatacgctga agaccgcatc aatgccatgc tgatgtacaa cgccaacatc atcggtcagg
      301 ctacccgcaa tgccaatcac cggtttatgg tacttcttcg ccacgtttgc gacaccaatc
      361 ggtaccttcc cgtgaatact ctggctgtca atacgccctt caccggtgat caccagcgta
      421 caatcgtgaa tatgttcctc cagattcagc gccgtagtga cgatttcaat accacttttc
      481 agttccgcac caagaaacgc cattagcgcc gcgcccatac cacccgcagc tcctgcaccg
      541 gggacatctt tcacatcaac atgcagcgct tttttaatga cctcggcata gtgagagagg
      601 ttattgtcca gctcaacaat catcgcttca ctggctccct tttgtgggcc aaagatgcgc
      661 gatgcgccgt tatcgcccac cagcggattg gtgacatcac aagcgacgcg aatgacgcaa
      721 tcttttaagc gcggatcgag gccggaaata tcaatatcat tcagagtatt aagactaccg
      781 ccgccaaaac caatttcatt gccgttggcg tc
//
