LOCUS       sequence-127393-         980 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  bacterial expression of G2K (d-glycerate 2-kinase).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 980)
  AUTHORS   Sims, P, Reed, G
  TITLE     Method for the enzymatic synthesis of 2-phospho-d-glycerate from 
            adenosine 5′-triphosphate and d-glycerate via d-glycerate-2-kinase
  JOURNAL   Journal of Molecular Catalysis B: Enzymatic 32 (2005) 77–81
REFERENCE   2  (bases 1 to 980)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Journal of 
            Molecular Catalysis B: Enzymatic 32 (2005) 77–81"
COMMENT     Sequence Label: pET-G2K
FEATURES             Location/Qualifiers
     source          1..980
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        complement(2..79)
                     /gene="lacI"
                     /label=lacI promoter
     primer_bind     285..304
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     primer_bind     388..407
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        388..406
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    407..431
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
ORIGIN
        1 cattcaccac cctgaattga ctctcttccg ggcgctatca tgccataccg cgaaaggttt
       61 tgcgccattc gatggtgtcc gggatctcga cgctctccct tatgcgactc ctgcattagg
      121 aagcagccca gtagtaggtt gaggccgttg agcaccgccg ccgcaaggaa tggtgcatgc
      181 aaggagatgg cgcccaacag tcccccggcc acggggcctg ccaccatacc cacgccgaaa
      241 caagcgctca tgagcccgaa gtggcgagcc cgatcttccc catcggtgat gtcggcgata
      301 taggcgccag caaccgcacc tgtggcgccg gtgatgccgg ccacgatgcg tccggcgtag
      361 aggatcgaga tctcgatccc gcgaaattaa tacgactcac tataggggaa ttgtgagcgg
      421 ataacaattc ccctctagaa ataattttgt ttaactttaa gaaggaattc aggagccctt
      481 caccatggcg tattgcaatc cgggcctgga atccaggccg aataagagaa acgccctccg
      541 gcgtcatgtg gtaacaggca taggtatgaa aatcgtaatc gccccagact cttataaaga
      601 aagtttatct gccagcgagg ttgcgcaggc gatagaaaaa ggatttcggg aaatttttcc
      661 tgatgcacag tacgtttctg ttccggttgc cgacggtggc gaaggaacgg tggaagcgat
      721 gattgcagcc acccaggggg ctgaacgtca cgcctgggtt acagggccgc tgggcgagaa
      781 agtgaatgcc agttggggga tctccggcga tggcaaaacc gcgtttattg aaatggcggc
      841 ggccagtggg ctggagctgg tacctgcgga aaaacgcgat ccactcgtga ccacttcacg
      901 cggcacaggc gagttaatcc tgcaggcgct ggagagcggt gcgacaaaca ttattatcgg
      961 cattggcggc agcgctacaa
//
