LOCUS       sequence-127397-         977 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  mtDNA destruction/production of rho-0 cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 977)
  AUTHORS   Spadafora D, Kozhukhar N, Chouljenko VN, Kousoulas KG, Alexeyev MF
  TITLE     Methods for Efficient Elimination of Mitochondrial DNA from Cultured
            Cells.
  JOURNAL   PLoS One. 2016 May 2;11(5):e0154684. doi: 
            10.1371/journal.pone.0154684. eCollection 2016.
  PUBMED    27136098
REFERENCE   2  (bases 1 to 977)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2016-05-2"; volume: "11(5):e0154684. doi"; pages: " 
            10.1371/journal.pone.0154684. eCollection 2016"
COMMENT     Sequence Label: pMA4008
FEATURES             Location/Qualifiers
     source          1..977
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      23..32
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(29..31,32..34,35..748)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(29..31,32..34,35..745)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(74..95)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(335..354)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     682..703
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     polyA_signal    749..870
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(786..805)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     840..859
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
ORIGIN
        1 attggtgtgc acctccaagc tcgccaccat ggtgagcaag ggcgaggagc tgttcaccgg
       61 ggtggtgccc atcctggtcg agctggacgg cgacgtaaac ggccacaagt tcagcgtgtc
      121 cggcgagggc gagggcgatg ccacctacgg caagctgacc ctgaagttca tctgcaccac
      181 cggcaagctg cccgtgccct ggcccaccct cgtgaccacc ctgacctacg gcgtgcagtg
      241 cttcagccgc taccccgacc acatgaagca gcacgacttc ttcaagtccg ccatgcccga
      301 aggctacgtc caggagcgca ccatcttctt caaggacgac ggcaactaca agacccgcgc
      361 cgaggtgaag ttcgagggcg acaccctggt gaaccgcatc gagctgaagg gcatcgactt
      421 caaggaggac ggcaacatcc tggggcacaa gctggagtac aactacaaca gccacaacgt
      481 ctatatcatg gccgacaagc agaagaacgg catcaaggtg aacttcaaga tccgccacaa
      541 catcgaggac ggcagcgtgc agctcgccga ccactaccag cagaacaccc ccatcggcga
      601 cggccccgtg ctgctgcccg acaaccacta cctgagcacc cagtccgccc tgagcaaaga
      661 ccccaacgag aagcgcgatc acatggtcct gctggagttc gtgaccgccg ccgggatcac
      721 tctcggcatg gacgagctgt acaagtaaaa cttgtttatt gcagcttata atggttacaa
      781 ataaagcaat agcatcacaa atttcacaaa taaagcattt ttttcactgc attctagttg
      841 tggtttgtcc aaactcatca atgtatctta tcatgtctgt atacccccat tttcgcggga
      901 aaactgaata agaggaagtg aaatctgaat aattttgtgt tactcatagc gcgtaatatt
      961 tgtctagggc cgcgggg
//
