LOCUS       sequence-127398-        1001 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  mtDNA destruction/production of rho-0 cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1001)
  AUTHORS   Spadafora D, Kozhukhar N, Chouljenko VN, Kousoulas KG, Alexeyev MF
  TITLE     Methods for Efficient Elimination of Mitochondrial DNA from Cultured
            Cells.
  JOURNAL   PLoS One. 2016 May 2;11(5):e0154684. doi: 
            10.1371/journal.pone.0154684. eCollection 2016.
  PUBMED    27136098
REFERENCE   2  (bases 1 to 1001)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2016-05-2"; volume: "11(5):e0154684. doi"; pages: " 
            10.1371/journal.pone.0154684. eCollection 2016"
COMMENT     Sequence Label: pMA4008
FEATURES             Location/Qualifiers
     source          1..1001
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     69..86
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     promoter        106..124
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     106..123
                     /label=SP6
                     /note="SP6 promoter, forward primer"
ORIGIN
        1 agtgggagtg gcaccttcca gggtcaagga aggcacgggg gaggggcaaa caacagatgg
       61 ctggcaacta gaaggcacag tcgaggctga tcagcgagct ctagcattta ggtgacacta
      121 tagaataggg ccctctagat gcatgctcga gtcagcgaga cgacctcccc gtcgtcggtg
      181 atgaggacgt tgtttcggca gcagcagggc cgggccccgg agaacgagag gcccatagct
      241 cggcgagcgt gtcgtcgaac gccaggcggc tgcttcgctg gatggcctta tagatctccg
      301 gatcgatgcg gacgggggta atgatcaggg cgatcggaac ggcctggttc gggagaatgg
      361 acgccttgct gggtcctgcg gccccgagag ccccggcgcc gtcctccagg cggaacgtta
      421 cgccctcctc cgcgctggtg cggtgcctgc cgataaacgt caccagatgc gggtgggggg
      481 ggcagtcggg gaagtggctg tcgagcacgt agccctgcac caagatctgc ttaaagttcg
      541 ggtgacgggg gttcgcgaag acgggctcgc ggcggaccag atccccggag ctccaggaca
      601 cgggggagat ggtgtggcgt ccgaggtcgg gggcgccaaa cagaagcacc tccgagacaa
      661 cgccgctatt taactccacc aaggcccgat ccgcggcgga gcaccgcctt ttttcgcccg
      721 aggcgtgggc ctctgaccag gcctggtctt gcgtgacgag agcctcctcc gggccgggga
      781 cgcgcccggg cgcgaagtat cgcacgctgg gcttcgggat cgaccggata aatgcccgga
      841 acgcctccgg ggaccggtgt gtcatcaagt cctcgtacgc ggaggccgtg gggtcgctgg
      901 ggtccatggg gtcgaaagcg tacttggccc ggcatttgac ctcgtaaaag gccagggggg
      961 tcttggggac tggggccagg tagccgtgaa tgtcccgagg a
//
