LOCUS       sequence-127400-        1002 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  mtDNA elimination/production of rho-0 cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1002)
  AUTHORS   Spadafora D, Kozhukhar N, Chouljenko VN, Kousoulas KG, Alexeyev MF
  TITLE     Methods for Efficient Elimination of Mitochondrial DNA from Cultured
            Cells.
  JOURNAL   PLoS One. 2016 May 2;11(5):e0154684. doi: 
            10.1371/journal.pone.0154684. eCollection 2016.
  PUBMED    27136098
REFERENCE   2  (bases 1 to 1002)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2016-05-2"; volume: "11(5):e0154684. doi"; pages: " 
            10.1371/journal.pone.0154684. eCollection 2016"
COMMENT     Sequence Label: pMA3790
FEATURES             Location/Qualifiers
     source          1..1002
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      31..40
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(37..39,40..42,43..756)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(37..39,40..42,43..753)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(82..103)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(343..362)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     690..711
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     promoter        complement(862..880)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(863..880)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(900..917)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
ORIGIN
        1 tttcaggtgt cgtgaagctt ggtaccggtc gccaccatgg tgagcaaggg cgaggagctg
       61 ttcaccgggg tggtgcccat cctggtcgag ctggacggcg acgtaaacgg ccacaagttc
      121 agcgtgtccg gcgagggcga gggcgatgcc acctacggca agctgaccct gaagttcatc
      181 tgcaccaccg gcaagctgcc cgtgccctgg cccaccctcg tgaccaccct gacctacggc
      241 gtgcagtgct tcagccgcta ccccgaccac atgaagcagc acgacttctt caagtccgcc
      301 atgcccgaag gctacgtcca ggagcgcacc atcttcttca aggacgacgg caactacaag
      361 acccgcgccg aggtgaagtt cgagggcgac accctggtga accgcatcga gctgaagggc
      421 atcgacttca aggaggacgg caacatcctg gggcacaagc tggagtacaa ctacaacagc
      481 cacaacgtct atatcatggc cgacaagcag aagaacggca tcaaggtgaa cttcaagatc
      541 cgccacaaca tcgaggacgg cagcgtgcag ctcgccgacc actaccagca gaacaccccc
      601 atcggcgacg gccccgtgct gctgcccgac aaccactacc tgagcaccca gtccgccctg
      661 agcaaagacc ccaacgagaa gcgcgatcac atggtcctgc tggagttcgt gaccgccgcc
      721 gggatcactc tcggcatgga cgagctgtac aagtaaagcg gccggccgcg actctagaat
      781 tccaactgag cgccggtcgc taccattacc aacttgtctg gtgtcaaaaa taataggcct
      841 actagttcta gagggcccta ttctatagtg tcacctaaat gctagagctc gctgatcagc
      901 ctcgactgtg ccttctagtt gccagccatc tgttgtttgc ccctcccccg tgccttcctt
      961 gaccctggaa ggtgccactc ccactgtcct ttcctaataa aa
//
