LOCUS       sequence-127402-        1044 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  bacterial expression of MBP-TEV-AICD.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1044)
  AUTHORS   Currinn H, Guscott B, Balklava Z, Rothnie A, Wassmer T
  TITLE     APP controls the formation of PI(3,5)P vesicles through its binding 
            of the PIKfyve complex.
  JOURNAL   Cell Mol Life Sci. 2015 Jul 28.
  PUBMED    26216398
REFERENCE   2  (bases 1 to 1044)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell Mol 
            Life Sci. 2015 Jul 28."
COMMENT     Sequence Label: pET28-MBP-TEV-AICD
FEATURES             Location/Qualifiers
     source          1..1044
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             30..47
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             57..74
                     /codon_start=1
                     /product="thrombin recognition and cleavage site"
                     /label=thrombin site
                     /translation="LVPRGS"
ORIGIN
        1 taagaaggag atataccatg ggcagcagcc atcatcatca tcatcacagc agcggcctgg
       61 tgccgcgcgg cagccatatg gctagcggta aaatcgaaga aggtaaactg gtaatctgga
      121 ttaacggcga taaaggctat aacggtctcg ctgaagtcgg taagaaattc gagaaagata
      181 ccggaattaa agtcaccgtt gagcatccgg ataaactgga agagaaattc ccacaggttg
      241 cggcaactgg cgatggccct gacattatct tctgggcaca cgaccgcttt ggtggctacg
      301 ctcaatctgg cctgttggct gaaatcaccc cggacaaagc gttccaggac aagctgtatc
      361 cgtttacctg ggatgccgta cgttacaacg gcaagctgat tgcttacccg atcgctgttg
      421 aagcgttatc gctgatttat aacaaagatc tgctgccgaa cccgccaaaa acctgggaag
      481 agatcccggc gctggataaa gaactgaaag cgaaaggtaa gagcgcgctg atgttcaacc
      541 tgcaagaacc gtacttcacc tggccgctga ttgctgctga cgggggttat gcgttcaagt
      601 atgaaaacgg caagtacgac attaaagacg tgggcgtgga taacgctggc gcgaaagcgg
      661 gtctgacctt cctggttgac ctgattaaaa acaaacacat gaatgcagac accgattact
      721 ccatcgcaga agctgccttt aataaaggcg aaacagcgat gaccatcaac ggcccgtggg
      781 catggtccaa catcgacacc agcaaagtga attatggtgt aacggtactg ccgaccttca
      841 agggtcaacc atccaaaccg ttcgttggcg tgctgagcgc aggtattaac gccgccagtc
      901 cgaacaaaga gctggcgaaa gagttcctcg aaaactatct gctgactgat gaaggtctgg
      961 aagcggttaa taaagacaaa ccgctgggtg ccgtagcgct gaagtcttac gaggaagagt
     1021 tggcgaaaga tccacgtatt gccg
//
