LOCUS       sequence-127403-         820 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  bacterial expression of MBP-TEV-AICD.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 820)
  AUTHORS   Currinn H, Guscott B, Balklava Z, Rothnie A, Wassmer T
  TITLE     APP controls the formation of PI(3,5)P vesicles through its binding 
            of the PIKfyve complex.
  JOURNAL   Cell Mol Life Sci. 2015 Jul 28.
  PUBMED    26216398
REFERENCE   2  (bases 1 to 820)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell Mol 
            Life Sci. 2015 Jul 28."
COMMENT     Sequence Label: pET28-MBP-TEV-AICD
FEATURES             Location/Qualifiers
     source          1..820
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(170..190)
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     primer_bind     complement(209..232)
                     /label=MBP-F
                     /note="Maltose binding protein, forward primer"
     regulatory      340..349
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 ggtggtggtg ctcgagctag ttctgcatct gctcaaagaa cttgtaggtt ggattttcgt
       61 agccgttctg ctgcatcttg gacaggtggc gctcctctgg ggtgacagcg gcgtcaacct
      121 ccaccacacc atgatgaatg gatgtgtact gtttcttctt accacaggat ccttggaagt
      181 atagattttc acccttggtg atacgagtct gcgcgtcttt cagggcttca tcgacagtct
      241 gacgaccgct ggcggcgttg atcaccgcag tacgcacggc ataccagaaa gcggacatct
      301 gcgggatgtt cggcatgatt tcacctttct gggcgttttc catggtggcg gcaatacgtg
      361 gatctttcgc caactcttcc tcgtaagact tcagcgctac ggcacccagc ggtttgtctt
      421 tattaaccgc ttccagacct tcatcagtca gcagatagtt ttcgaggaac tctttcgcca
      481 gctctttgtt cggactggcg gcgttaatac ctgcgctcag cacgccaacg aacggtttgg
      541 atggttgacc cttgaaggtc ggcagtaccg ttacaccata attcactttg ctggtgtcga
      601 tgttggacca tgcccacggg ccgttgatgg tcatcgctgt ttcgccttta ttaaaggcag
      661 cttctgcgat ggagtaatcg gtgtctgcat tcatgtgttt gtttttaatc aggtcaacca
      721 ggaaggtcag acccgctttc gcgccagcgt tatccacgcc cacgtcttta atgtcgtact
      781 tgccgttttc atacttgaac gcataacccc cgtcagcagc
//
