LOCUS       sequence-127406-         752 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  IPTG-inducible CRISPRi vector targeting nonsense sequence (NS), 
            pNBU2 backbone, AmpR.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 752)
  AUTHORS   Mimee M, Tucker AC, Voigt CA, Lu TK
  TITLE     Programming a Human Commensal Bacterium, Bacteroides 
            thetaiotaomicron, to Sense and Respond to Stimuli in the Murine Gut 
            Microbiota.
  JOURNAL   Cell Syst. 2015 Jul 29;1(1):62-71. doi: 10.1016/j.cels.2015.06.001.
  PUBMED    26918244
REFERENCE   2  (bases 1 to 752)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cels.2015.06"; journalName: "Cell Syst"; date: 
            "2015-07-29- 29"; volume: "1"; issue: "1"; pages: "62-71"
COMMENT     Sequence Label: pMM710
FEATURES             Location/Qualifiers
     source          1..752
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     misc_RNA        125..200
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     primer_bind     complement(664..683)
                     /label=LacI-R
                     /note="LacI, reverse primer"
ORIGIN
        1 ccaaaaaaat cctagcaccc tgcagcagta ctgcttgacc ataagaacaa aaaaacttcc
       61 gataaagttt ggaagataaa gctaaaagtt cttatctttg cagtctggaa tgaattggcc
      121 tatggtttta gagctagaaa tagcaagtta aaataaggct agtccgttat caacttgaaa
      181 aagtggcacc gagtcggtgc tttttttcta gatgatgatt aaggatcttg cgtacaattt
      241 gacgatcgtt tcacgctaag atgctggcta attgcctatc ttccagtgat ggaacagcat
      301 ttgtgcattg gctgcaacaa tagccttgat ctggaagaag caatgaaagc tgctgttaag
      361 tctccgaatc aggtattgtt cctgacaggt gtattcccat ccggtaaacg cggatacttt
      421 gcagttgatc tgactcagga ataaattata aattaaggta agaagattgt aggataagct
      481 aatgaaatag aaaaaggatg ccgtcacaca acttgtcggc attctttttt gttttattag
      541 ttgaaaatat agtgaaaaag ttgcctaaat atgtatgtta acaaattatt tgtcgtaact
      601 ttgcactcca aatctgtttt taacatatgg cactagtggt gaatgtgaaa ccagtaacgt
      661 tatacgatgt cgcagagtat gccggtgtct cttatcagac cgtttcccgc gtggtgaacc
      721 aggccagcca cgtttctgcg aaaacgcggg aa
//
