LOCUS       sequence-127412-         472 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Express Streptococcus pyogenes Cas9 carrying two C-terminal SV40 
            NLS.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 472)
  AUTHORS   Lin S, Staahl BT, Alla RK, Doudna JA
  TITLE     Enhanced homology-directed human genome engineering by controlled 
            timing of CRISPR/Cas9 delivery.
  JOURNAL   Elife. 2014 Dec 15;3:e04766. doi: 10.7554/eLife.04766.
  PUBMED    25497837
REFERENCE   2  (bases 1 to 472)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Elife."; 
            date: "2014-12-15"; volume: "3:e04766. doi"; pages: " 
            10.7554/eLife.04766"
COMMENT     Sequence Label: pMJ915
FEATURES             Location/Qualifiers
     source          1..472
                     /mol_type="genomic DNA"
                     /organism="unspecified"
ORIGIN
        1 ggataagaaa tactcaatag gcttagatat cggcacaaat agcgtcggat gggcggtgat
       61 cactgatgaa tataaggttc cgtctaaaaa gttcaaggtt ctgggaaata cagaccgcca
      121 cagtatcaaa aaaaatctta taggggctct tttatttgac agtggagaga cagcggaagc
      181 gactcgtctc aaacggacag ctcgtagaag gtatacacgt cggaagaatc gtatttgtta
      241 tctacaggag attttttcaa atgagatggc gaaagtagat gatagtttct ttcatcgact
      301 tgaagagtct tttttggtgg aagaagacaa gaagcatgaa cgtcatccta tttttggaaa
      361 tatagtagat gaagttgctt atcatgagaa atatccaact atctatcatc tgcgaaaaaa
      421 attggtagat tctactgata aagcggattt gcgcttaatc tatttggcct ta
//
