LOCUS       sequence-127414-         775 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Express Streptococcus pyogenes Cas9 carrying two C-terminal SV40 
            NLS.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 775)
  AUTHORS   Lin S, Staahl BT, Alla RK, Doudna JA
  TITLE     Enhanced homology-directed human genome engineering by controlled 
            timing of CRISPR/Cas9 delivery.
  JOURNAL   Elife. 2014 Dec 15;3:e04766. doi: 10.7554/eLife.04766.
  PUBMED    25497837
REFERENCE   2  (bases 1 to 775)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Elife."; 
            date: "2014-12-15"; volume: "3:e04766. doi"; pages: " 
            10.7554/eLife.04766"
COMMENT     Sequence Label: pMJ915
FEATURES             Location/Qualifiers
     source          1..775
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(98..118)
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             complement(125..145)
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
ORIGIN
        1 gttagcagcc ggatcagcgc gcacgcgtgg taccggccgg ccaccaggtg gcgcgccgcg
       61 atcgcggatc cgttatccac ttccaatgtt attaatcaac ttttcgtttc tttttaggat
      121 cctcaacttt tcgttttttc ttaggtgacc cgtcacctcc tagctgactc aaatcaatgc
      181 gtgtttcata aagaccagtg atggattgat ggataagagt ggcatctaaa acttcttttg
      241 tagacgtata tcgtttacga tcaattgttg tatcaaaata tttaaaagca gcgggagctc
      301 caagattcgt caacgtaaat aaatgaataa tattttctgc ttgttcacgt attggtttgt
      361 ctctatgttt gttatatgca ctaagaactt tatctaaatt ggcatctgct aaaataacac
      421 gcttagaaaa ttcactgatt tgctcaataa tctcatctaa ataatgctta tgctgctcca
      481 caaacaattg tttttgttcg ttatcttctg gactaccctt caacttttca taatgactag
      541 ctaaatataa aaaattcaca tatttgcttg gcagagccag ctcatttcct ttttgtaatt
      601 ctccggcact agccagcatc cgtttacgac cgttttctaa ctcaaaaaga ctatatttag
      661 gtagtttaat gattaagtct tttttaactt ccttatatcc tttagcttct aaaaagtcaa
      721 tcggattttt ttcaaaggaa cttctttcca taattgtgat ccctagtaac tcttt
//
