LOCUS       sequence-127415-         977 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  KanR with FRT sites + ptet::OmpC::T7RNAP::T1 terminator + ColE.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 977)
  AUTHORS   Shin JE, Lin C, Lim HN
  TITLE     Horizontal transfer of DNA methylation patterns into bacterial 
            chromosomes.
  JOURNAL   Nucleic Acids Res. 2016 Apr 15. pii: gkw230.
  PUBMED    27084942
REFERENCE   2  (bases 1 to 977)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic 
            Acids Res. 2016 Apr 15. pii: gkw230."
COMMENT     Sequence Label: pHL 1477
FEATURES             Location/Qualifiers
     source          1..977
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    complement(390..423)
                     /label=FRT (minimal)
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="supports FLP-mediated excision but not integration 
                     (Turan and Bode, 2011)"
     primer_bind     494..512
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     protein_bind    533..551
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    558..576
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
ORIGIN
        1 tgcatccatc ttgttcaatc atgcgaaacg atcctcatcc tgtctcttga tcagatcttg
       61 atcccctgcg ccatcagatc cttggcggca agaaagccat ccagtttact ttgcagggct
      121 tcccaacctt accagagggc gccccagctg gcaattccgg ttcgcttgct gtccataaaa
      181 ccgcccagtc tagctatcgc catgtaagcc cactgcaagc tacctgcttt ctctttgcgc
      241 ttgcgttttc ccttgtccag atagcccagt agctgacatt catccggggt cagcaccgtt
      301 tctgcggact ggctttctac gtgttccgct tcctttagca gcccttgcgc cctgagtgct
      361 tgcggcagcg tgagcttcaa aagcgctctg aagttcctat actttctaga gaataggaac
      421 ttcgaactgc aggtcgacgg atccccggaa tgacgtctaa gaaaccatta ttatcatgac
      481 attaacctat aaaaataggc gtatcacgag gccctttcgt cttcacctcg agtccctatc
      541 agtgatagag attgacatcc ctatcagtga tagagatact gtcgacttgc cgactgatta
      601 atgagggtta atcagtatgc agtggcataa aaaagcaaat aaaggcatat aacagagggt
      661 taataacatg aaagttaaag tactgtccct cctggtccca gctagcaaca tgcatgcaca
      721 cgattaacat cgctaagaac gacttctctg acatcgaact ggctgctatc ccgttcaaca
      781 ctctggctga ccattacggt gagcgtttag ctcgcgaaca gttggccctt gagcatgagt
      841 cttacgagat gggtgaagca cgcttccgca agatgtttga gcgtcaactt aaagctggtg
      901 aggttgcgga taacgctgcc gccaagcctc tcatcactac cctactccct aagatgattg
      961 cacgcatcaa cgactgg
//
