LOCUS       sequence-127424-         475 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  to knockdown endogenous miR144 levels; lentiviral vector containing 
            8x copies of bulged trap sequences cloned 3' to GFP under the 
            control of a biPGK promoter .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 475)
  AUTHORS   Kim M, Tan YS, Cheng WC, Kingsbury TJ, Heimfeld S, Civin CI
  TITLE     MIR144 and MIR451 regulate human erythropoiesis via RAB14.
  JOURNAL   Br J Haematol. 2014 Oct 14. doi: 10.1111/bjh.13164.
  PUBMED    25312678
REFERENCE   2  (bases 1 to 475)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Br J 
            Haematol. 2014 Oct 14. doi: 10.1111/bjh.13164."
COMMENT     Sequence Label: miR-451 Trap
FEATURES             Location/Qualifiers
     source          1..475
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(37..48)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
ORIGIN
        1 ggattctgcg cgggacgtcc ttctgcctac gtcccttcgg ccctcaatcc agcggacctt
       61 ccttcccgcg gcctgctgcc ggctctagaa ttcaactcag taaccaaacg gttttagaga
      121 acttaactca gtaaccaaac ggtttagaga acttaaactc agtaaccaaa cggtttgaac
      181 ttagagaact cagtaaccaa acggtttctt agagaacaac tcagtaacca aacggtttag
      241 agaacttaaa ctcagtaacc aaacggtttt agagaactta actcagtaac caaacggttt
      301 cttagagaac aactcagtaa ccaaacggtt taccggtgaa ttcgatatca agggtacctt
      361 taagaccaat gacttacaag gcagctgtag atcttagcca ctttttaaaa gaaaaggggg
      421 gactggaagg gctaattcac tcccaacgaa gacaagatct gctttttgct tgtac
//
