LOCUS       sequence-127427-        1000 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses rat Cx43 (Gja1 CDS) with a 7 amino acid linker on the 
            C-terminus of the Connexin linking to monomerized superfolder Green 
            Fluorescent Protein. Expression driven by CMV promoter..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1000)
  AUTHORS   Stout RF Jr, Snapp EL, Spray DC
  TITLE     Connexin Type and Fluorescent Protein-fusion Tag Determine 
            Structural Stability of Gap Junction Plaques.
  JOURNAL   J Biol Chem. 2015 Aug 11. pii: jbc.M115.659979.
  PUBMED    26265468
REFERENCE   2  (bases 1 to 1000)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Biol 
            Chem. 2015 Aug 11. pii: jbc.M115.659979."
COMMENT     Sequence Label: Cx43-msfGFP
FEATURES             Location/Qualifiers
     source          1..1000
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(join(1..714,715..717,718..720))
                     /codon_start=1
                     /product="GFP variant that folds robustly even when fused 
                     to poorly folded proteins (Pedelacq et al., 2006)"
                     /label=superfolder GFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVRGEGEGDATNGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKRHDFFKSAMPEGYVQERTISFKDD
                     GTYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNFNSHNVYITADKQKNGIK
                     ANFKIRHNVEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSKLSKDPNEKRDHMVLL
                     EFVTAAGITHGMDELYK"
     primer_bind     complement(46..67)
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     654..675
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     regulatory      717..726
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 ttacttgtac agctcgtcca tgccgtgagt gatcccggcg gcggtcacga actccagcag
       61 gaccatgtga tcgcgcttct cgttggggtc tttgctcagc ttggactggg tgctcaggta
      121 gtggttgtcg ggcagcagca cggggccgtc gccgatgggg gtgttctgct ggtagtggtc
      181 ggcgagctgc acgctgccgt cctccacgtt gtggcggatc ttgaagttgg ccttgatgcc
      241 gttcttctgc ttgtcggcgg tgatatagac gttgtggctg ttgaagttgt actccagctt
      301 gtgccccagg atgttgccgt cctccttgaa gtcgatgccc ttcagctcga tgcggttcac
      361 cagggtgtcg ccctcgaact tcacctcggc gcgggtcttg taggtgccgt cgtccttgaa
      421 gctgatggtg cgctcctgga cgtagccttc gggcatggcg gacttgaaga agtcgtggcg
      481 cttcatgtgg tcggggtagc ggctgaagca ctgcacgccg taggtcaggg tggtcacgag
      541 ggtgggccag ggcacgggca gcttgccggt ggtgcagatg aacttcaggg tcagcttgcc
      601 gttggtggca tcgccctcgc cctcgccgcg cacgctgaac ttgtggccgt ttacgtcgcc
      661 gtccagctcg accaggatgg gcaccacccc ggtgaacagc tcctcgccct tgctcaccat
      721 ggtggcgacc ggtggatccc gggcggaaat ctccaggtca tcaggccgag gcctgctgct
      781 ggcgcggctg ctggctctgc tggaaggtcg ttggtccacg atggctaatg gctggagttc
      841 atgtccagca gcaacttttt tggcattctg gttgtcgtcg gggaaatcga acggctgggc
      901 gtgcgagttg gagatggtgc ttccggcctg ccccatgcga ttttgctctg cgctgtagtt
      961 cgcccagttt tgctcgctag cttgcttgtt gtaattgcgg
//
