LOCUS       sequence-127434-        1055 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses rat Cx26 (Gjb2 CDS) with a 7 amino acid linker on the 
            C-terminus of the Connexin linking to monomerized superfolder Green 
            Fluorescent Protein. Expression driven by CMV promoter..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1055)
  AUTHORS   Stout RF Jr, Snapp EL, Spray DC
  TITLE     Connexin Type and Fluorescent Protein-fusion Tag Determine 
            Structural Stability of Gap Junction Plaques.
  JOURNAL   J Biol Chem. 2015 Aug 11. pii: jbc.M115.659979.
  PUBMED    26265468
REFERENCE   2  (bases 1 to 1055)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Biol 
            Chem. 2015 Aug 11. pii: jbc.M115.659979."
COMMENT     Sequence Label: Cx26-msfGFP
FEATURES             Location/Qualifiers
     source          1..1055
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      699..708
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     primer_bind     complement(750..771)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
ORIGIN
        1 ctagcatgga ttggggcaca ctacagagca tcctcggggg tgtcaacaag cactccacca
       61 gcattgggaa aatctggctc actgtcctct tcatcttccg catcatgatc ctcgtggtgg
      121 ccgcgaagga ggtgtgggga gatgagcaag ccgattttgt ttgcaacact ctccagcctg
      181 gctgtaagaa tgtgtgctac gaccactact tccccatctc tcacatccgg ctctgggctc
      241 tgcagctgat catggtgtcc acgccggccc tcctggtagc tatgcacgtg gcctaccgga
      301 gacacgaaaa gaaacggaag ttcatgaagg gagagataaa gaacgagttt aaggacatcg
      361 aagagatcaa aacccagaag gtccgtatcg aagggtccct gtggtggacc tacaccacca
      421 gcatcttctt ccgggtcatc ttcgaagctg tcttcatgta tgtcttttac atcatgtaca
      481 atggcttctt catgcagcgt ctggtgaagt gtaacgcctg gccttgtccc aatacagtgg
      541 actgcttcat ttccaggccc acagaaaaga ctgtcttcac ggtgttcatg atctctgtgt
      601 ctggaatttg catcctgcta aacatcacag agctgtgcta tctgttcatt aggtattgct
      661 cagggaagtc caaaagacca gtcgcggatc caccggtcgc caccatggtg agcaagggcg
      721 aggagctgtt caccggggtg gtgcccatcc tggtcgagct ggacggcgac gtaaacggcc
      781 acaagttcag cgtgcgcggc gagggcgagg gcgatgccac caacggcaag ctgaccctga
      841 agttcatctg caccaccggc aagctgcccg tgccctggcc caccctcgtg accaccctga
      901 cctacggcgt gcagtgcttc agccgctacc ccgaccacat gaagcgccac gacttcttca
      961 agtccgccat gcccgaaggc tacgtccagg agcgcaccat cagcttcaag gacgacggca
     1021 cctacaagac ccgcgccgag gtgaagttcg agggc
//
