LOCUS       sequence-127437-         490 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  read-outloxN mCherry to GFP switch for integration on ttTi5605, Mos 
            Chr II.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 490)
  AUTHORS   Ruijtenberg S, van den Heuvel S
  TITLE     G1/S Inhibitors and the SWI/SNF Complex Control Cell-Cycle Exit 
            during Muscle Differentiation.
  JOURNAL   Cell. 2015 Jul 16;162(2):300-13. doi: 10.1016/j.cell.2015.06.013. 
            Epub 2015 Jul 2.
  PUBMED    26144318
REFERENCE   2  (bases 1 to 490)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2015.06.013"; journalName: "Cell"; date: 
            "2015-07-16- 16"; volume: "162"; issue: "2"; pages: "300-13"
COMMENT     Sequence Label: pSR06
FEATURES             Location/Qualifiers
     source          1..490
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(335..355)
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     protein_bind    384..417
                     /label=loxN
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (AGGTATAC) (Shaw et al., 2021). loxN sites are 
                     compatible with each other, but incompatible with loxP or 
                     lox2272 sites."
ORIGIN
        1 aggagagctt gaggtagtct gggatgtcgg ctgggtgctt gacgtaggcc tttgatccgt
       61 acatgaattg tggggagagg atgtcccagg cgaatgggag tggtcctccc ttggtgacct
      121 gaaaatttaa ataatcaggg ttagttagta tatatatgtt taaacttacc ttgagcttgg
      181 cggtttgggt tccctcgtat ggacgtccct ctccctctcc ctcgatctcg aactcgtgtc
      241 cgttgactga tccctccatg tggaccttga aacgcatgaa ctccttgatg atggccatgt
      301 tgtcctcctc tcccttggac attttttcta ccggtacctt acgcttcttc tttggagcag
      361 tcattttttc tggatcctct agaataactt cgtataaggt atactatacg aagttatttt
      421 tattccactt gttgagcggg gctggctgaa aatatgtgaa ttgagaaaat aaatagtata
      481 tttgaaagaa
//
