LOCUS       sequence-127441-         297 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Parental plasmid for transcription of RNAs with a HdV/M-domain 
            3'-tag for native purification.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 297)
  AUTHORS   Kieft JS, Batey RT
  TITLE     A general method for rapid and nondenaturing purification of RNAs.
  JOURNAL   RNA. 2004 Jun;10(6):988-95.
  PUBMED    15146082
REFERENCE   2  (bases 1 to 297)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "RNA."; 
            date: "2004-06"; volume: "10(6)"; pages: "988-95"
COMMENT     Sequence Label: pRAV12
FEATURES             Location/Qualifiers
     source          1..297
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     7..26
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        7..25
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     terminator      244..291
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
ORIGIN
        1 gaattctaat acgactcact atagggtcgc tcaaacatta agtggtgttg tgcgaaaaga
       61 atctcgaaga caagccggcc atggtcccag cctcctcgct ggcggccggt ggcaacatgc
      121 ttcggcatgg tgaatgggac ctctagactg tgcatcgggt caggactgaa aggtagcagc
      181 cctgggcagt tttttcctgt gcatcgggtc aggaccttcg ggtagcagcc ctgggcagga
      241 tccctagcat aaccccttgg ggcctctaaa cgggtcttga ggggtttttt gaagctt
//
