LOCUS       sequence-127447-         650 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 650)
  AUTHORS   Alberti S, Gitler AD, Lindquist S
  TITLE     A suite of Gateway cloning vectors for high-throughput genetic 
            analysis in Saccharomyces cerevisiae.
  JOURNAL   Yeast. 2007 Oct . 24(10):913-9.
  PUBMED    17583893
REFERENCE   2  (bases 1 to 650)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Yeast. 2007
            Oct . 24(10):913-9."
COMMENT     Sequence Label: pAG423GAL-ccdB-ECFP
FEATURES             Location/Qualifiers
     source          1..650
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    90..137
                     /label=FRT
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="FLP-mediated recombination occurs in the 8-bp core 
                     sequence TCTAGAAA (Turan and Bode, 2011)."
ORIGIN
        1 cacgcagata taggtgcgac gtgaacagtg agctgtatgt gcgcagctcg cgttgcattt
       61 tcggaagcgc tcgttttcgg aaacgctttg aagttcctat tccgaagttc ctattctcta
      121 gaaagtatag gaacttcaga gcgcttttga aaaccaaaag cgctctgaag acgcactttc
      181 aaaaaaccaa aaacgcaccg gactgtaacg agctactaaa atattgcgaa taccgcttcc
      241 acaaacattg ctcaaaagta tctctttgct atatatctct gtgctatatc cctatataac
      301 ctacccatcc acctttcgct ccttgaactt gcatctaaac tcgacctcta cattttttat
      361 gtttatctct agtattactc tttagacaaa aaaattgtag taagaactat tcatagagtg
      421 aatcgaaaac aatacgaaaa tgtaaacatt tcctatacgt agtatataga gacaaaatag
      481 aagaaaccgt tcataatttt ctgaccaatg aagaatcatc aacgctatca ctttctgttc
      541 acaaagtatg cgcaatccac atcggtatag aatataatcg gggatgcctt tatcttgaaa
      601 aaatgcaccc gcagcttcgc tagtaatcag taaacgcggg aagtggagtc
//
