LOCUS       sequence-127450-         485 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 485)
  AUTHORS   Alberti S, Gitler AD, Lindquist S
  TITLE     A suite of Gateway cloning vectors for high-throughput genetic 
            analysis in Saccharomyces cerevisiae.
  JOURNAL   Yeast. 2007 Oct . 24(10):913-9.
  PUBMED    17583893
REFERENCE   2  (bases 1 to 485)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Yeast. 2007
            Oct . 24(10):913-9."
COMMENT     Sequence Label: pAG423GPD-ccdB-ECFP
FEATURES             Location/Qualifiers
     source          1..485
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    41..88
                     /label=FRT
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="FLP-mediated recombination occurs in the 8-bp core 
                     sequence TCTAGAAA (Turan and Bode, 2011)."
ORIGIN
        1 gcgttgcatt ttcggaagcg ctcgttttcg gaaacgcttt gaagttccta ttccgaagtt
       61 cctattctct agaaagtata ggaacttcag agcgcttttg aaaaccaaaa gcgctctgaa
      121 gacgcacttt caaaaaacca aaaacgcacc ggactgtaac gagctactaa aatattgcga
      181 ataccgcttc cacaaacatt gctcaaaagt atctctttgc tatatatctc tgtgctatat
      241 ccctatataa cctacccatc cacctttcgc tccttgaact tgcatctaaa ctcgacctct
      301 acatttttta tgtttatctc tagtattact ctttagacaa aaaaattgta gtaagaacta
      361 ttcatagagt gaatcgaaaa caatacgaaa atgtaaacat ttcctatacg tagtatatag
      421 agacaaaata gaagaaaccg ttcataattt tctgaccaat gaagaatcat caacgctatc
      481 acttt
//
