LOCUS       sequence-127453-         810 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Membrane Receptor, Excitation: N/A, Emission: N/A.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 810)
  TITLE     Davidson plasmids
REFERENCE   2  (bases 1 to 810)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: ALK1-C-18
FEATURES             Location/Qualifiers
     source          1..810
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      45..54
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             51..596
                     /codon_start=1
                     /product="human O6-alkylguanine-DNA-alkyltransferase 
                     variant that forms covalent bonds with benzylguanine 
                     derivatives"
                     /label=SNAP-tag(R)
                     /note="fast-reacting variant, optimized for mammalian 
                     expression"
                     /translation="MDKDCEMKRTTLDSPLGKLELSGCEQGLHRIIFLGKGTSAADAVE
                     VPAPAAVLGGPEPLMQATAWLNAYFHQPEAIEEFPVPALHHPVFQQESFTRQVLWKLLK
                     VVKFGEVISYSHLAALAGNPAATAAVKTALSGNPVPILIPCHRVVQGDLDVGGYEGGLA
                     VKEWLLAHEGHRLGKPGLG"
ORIGIN
        1 gagctggttt agtgaaccgt tcagatccgc tagcgctacc ggtcgccacc atggacaaag
       61 actgcgaaat gaagcgcacc accctggata gccctctggg caagctggaa ctgtctgggt
      121 gcgaacaggg cctgcaccgt atcatcttcc tgggcaaagg aacatctgcc gccgacgccg
      181 tggaagtgcc tgccccagcc gccgtgctgg gcggaccaga gccactgatg caggccaccg
      241 cctggctcaa cgcctacttt caccagcctg aggccatcga ggagttccct gtgccagccc
      301 tgcaccaccc agtgttccag caggagagct ttacccgcca ggtgctgtgg aaactgctga
      361 aagtggtgaa gttcggagag gtcatcagct acagccacct ggccgccctg gccggcaatc
      421 ccgccgccac cgccgccgtg aaaaccgccc tgagcggaaa tcccgtgccc attctgatcc
      481 cctgccaccg ggtggtgcag ggcgacctgg acgtgggggg ctacgagggc gggctcgccg
      541 tgaaagagtg gctgctggcc cacgagggcc acagactggg caagcctggg ctgggttccg
      601 gactcagatc tcgagctcaa gcttcgaatt cagggtctgg aagtggtagc accttgggct
      661 cccccaggaa aggccttctg atgctgctga tggccttggt gacccaggga gaccctgtga
      721 agccgtctcg gggcccgctg gtgacctgca cgtgtgagag cccacattgc aaggggccta
      781 cctgccgggg ggcctggtgc acagtagtgc
//
