LOCUS       sequence-127456-         741 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 741)
  AUTHORS   Alberti S, Gitler AD, Lindquist S
  TITLE     A suite of Gateway cloning vectors for high-throughput genetic 
            analysis in Saccharomyces cerevisiae.
  JOURNAL   Yeast. 2007 Oct . 24(10):913-9.
  PUBMED    17583893
REFERENCE   2  (bases 1 to 741)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Yeast. 2007
            Oct . 24(10):913-9."
COMMENT     Sequence Label: pAG424GPD-ccdB-HA
FEATURES             Location/Qualifiers
     source          1..741
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    248..295
                     /label=FRT
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="FLP-mediated recombination occurs in the 8-bp core 
                     sequence TCTAGAAA (Turan and Bode, 2011)."
ORIGIN
        1 gtgctgaagg aagcatacga taccccgcat ggaatgggat aatatcacag gaggtactag
       61 actacctttc atcctacata aatagacgca tataagtacg catttaagca taaacacgca
      121 ctatgccgtt cttctcatgt atatatatat acaggcaaca cgcagatata ggtgcgacgt
      181 gaacagtgag ctgtatgtgc gcagctcgcg ttgcattttc ggaagcgctc gttttcggaa
      241 acgctttgaa gttcctattc cgaagttcct attctctaga aagtatagga acttcagagc
      301 gcttttgaaa accaaaagcg ctctgaagac gcactttcaa aaaaccaaaa acgcaccgga
      361 ctgtaacgag ctactaaaat attgcgaata ccgcttccac aaacattgct caaaagtatc
      421 tctttgctat atatctctgt gctatatccc tatataacct acccatccac ctttcgctcc
      481 ttgaacttgc atctaaactc gacctctaca ttttttatgt ttatctctag tattactctt
      541 tagacaaaaa aattgtagta agaactattc atagagtgaa tcgaaaacaa tacgaaaatg
      601 taaacatttc ctatacgtag tatatagaga caaaatagaa gaaaccgttc ataattttct
      661 gaccaatgaa gaatcatcaa cgctatcact ttctgttcac aaagtatgcg caatccacat
      721 cggtatagaa tataatcggg g
//
