LOCUS       sequence-127459-        1018 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses human Cx30 (GJB6 CDS) with a 7 amino acid linker on the 
            C-terminus of the Connexin linking to monomerized superfolder Green 
            Fluorescent Protein. Expression driven by CMV promoter..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1018)
  AUTHORS   Stout RF Jr, Snapp EL, Spray DC
  TITLE     Connexin Type and Fluorescent Protein-fusion Tag Determine 
            Structural Stability of Gap Junction Plaques.
  JOURNAL   J Biol Chem. 2015 Aug 11. pii: jbc.M115.659979.
  PUBMED    26265468
REFERENCE   2  (bases 1 to 1018)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Biol 
            Chem. 2015 Aug 11. pii: jbc.M115.659979."
COMMENT     Sequence Label: Cx30-msfGFP
FEATURES             Location/Qualifiers
     source          1..1018
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      19..28
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     promoter        complement(858..909)
                     /label=CMVd2 promoter
                     /note="truncated human cytomegalovirus (CMV) immediate 
                     early promoter that yields moderately reduced expression 
                     relative to the full-length promoter"
     primer_bind     complement(887..907)
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
ORIGIN
        1 gctcctcgcc cttgctcacc atggtggcga ccggtggatc cgggtagctt gggaaacctg
       61 tgattgcatt ttgaccacta tctgaaatca gctcattcat ttcattctgc ttactctcct
      121 ttagggcatg attggggtga tttttttgcg tctgtgctct ctttgatctc ctaaaacaca
      181 ctttcagcag caggtagcac aactctgcca cgttaagcag catgcaaatc acagacgcag
      241 aaatcataaa aatggtaaac acggtcttct ctgttggcct agaaataaag cagtcaacaa
      301 ggttggggca ggggtcaatc ccacatttca acacccaggg caggtggtac ccattgtaaa
      361 ggaagtaaaa cacatacata aaggctgctt caaagatgat tcggaaaaag atgctgctgg
      421 tgtacgtcca ccacagcgac ccctctatcc gaaccttctg ctttttaatg tcctctatgt
      481 ctttgaaatc attcctcttc tctcctcgcc tgaacttgcg agtggtttcg tgcctgtagt
      541 aggccacatg catggccacc agcagcgctg gggtggagac gaagatcagc tggagggccc
      601 acagccggat gtgggacacc gggaaaaagt ggtcatagca cacatttttg catcccggtt
      661 gcagtgtgtt gcagacgaag tcctcttgct cgtcacccca cacttcctgg gcagccacca
      721 cgaggatcat gactcggaaa ataaagatga ctgtgatcca caccttcccg atgctggtgg
      781 agtgtttgtt gacacccccg atgaaagtgt gcagcgtccc ccaatccatg ctagcggatc
      841 tgacggttca ctaaaccagc tctgcttata tagacctccc accgtacacg cctaccgccc
      901 atttgcgtca atggggcgga gttgttacga cattttggaa agtcccgttg atttggtgcc
      961 aaacaaactc ccattgacgt caatggggtg gagacttgga aatccccgtg agtcaaac
//
