LOCUS       sequence-127460-        1013 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses human Cx30 (GJB6 CDS) with a 7 amino acid linker on the 
            C-terminus of the Connexin linking to monomerized superfolder Green 
            Fluorescent Protein. Expression driven by CMV promoter..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1013)
  AUTHORS   Stout RF Jr, Snapp EL, Spray DC
  TITLE     Connexin Type and Fluorescent Protein-fusion Tag Determine 
            Structural Stability of Gap Junction Plaques.
  JOURNAL   J Biol Chem. 2015 Aug 11. pii: jbc.M115.659979.
  PUBMED    26265468
REFERENCE   2  (bases 1 to 1013)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Biol 
            Chem. 2015 Aug 11. pii: jbc.M115.659979."
COMMENT     Sequence Label: Cx30-msfGFP
FEATURES             Location/Qualifiers
     source          1..1013
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(join(6..719,720..722,723..725))
                     /codon_start=1
                     /product="GFP variant that folds robustly even when fused 
                     to poorly folded proteins (Pedelacq et al., 2006)"
                     /label=superfolder GFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVRGEGEGDATNGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKRHDFFKSAMPEGYVQERTISFKDD
                     GTYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNFNSHNVYITADKQKNGIK
                     ANFKIRHNVEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSKLSKDPNEKRDHMVLL
                     EFVTAAGITHGMDELYK"
     primer_bind     complement(51..72)
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     659..680
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     regulatory      722..731
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 ccgctttact tgtacagctc gtccatgccg tgagtgatcc cggcggcggt cacgaactcc
       61 agcaggacca tgtgatcgcg cttctcgttg gggtctttgc tcagcttgga ctgggtgctc
      121 aggtagtggt tgtcgggcag cagcacgggg ccgtcgccga tgggggtgtt ctgctggtag
      181 tggtcggcga gctgcacgct gccgtcctcc acgttgtggc ggatcttgaa gttggccttg
      241 atgccgttct tctgcttgtc ggcggtgata tagacgttgt ggctgttgaa gttgtactcc
      301 agcttgtgcc ccaggatgtt gccgtcctcc ttgaagtcga tgcccttcag ctcgatgcgg
      361 ttcaccaggg tgtcgccctc gaacttcacc tcggcgcggg tcttgtaggt gccgtcgtcc
      421 ttgaagctga tggtgcgctc ctggacgtag ccttcgggca tggcggactt gaagaagtcg
      481 tggcgcttca tgtggtcggg gtagcggctg aagcactgca cgccgtaggt cagggtggtc
      541 acgagggtgg gccagggcac gggcagcttg ccggtggtgc agatgaactt cagggtcagc
      601 ttgccgttgg tggcatcgcc ctcgccctcg ccgcgcacgc tgaacttgtg gccgtttacg
      661 tcgccgtcca gctcgaccag gatgggcacc accccggtga acagctcctc gcccttgctc
      721 accatggtgg cgaccggtgg atccgggtag cttgggaaac ctgtgattgc attttgacca
      781 ctatctgaaa tcagctcatt catttcattc tgcttactct cctttagggc atgattgggg
      841 tgattttttt gcgtctgtgc tctctttgat ctcctaaaac acactttcag cagcaggtag
      901 cacaactctg ccacgttaag cagcatgcaa atcacagacg cagaaatcat aaaaatggta
      961 aacacggtct tctctgttgg cctagaaata aagcagtcaa caaggttggg gca
//
