LOCUS       sequence-127464-        8069 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8069)
  AUTHORS   Alberti S, Gitler AD, Lindquist S
  TITLE     A suite of Gateway cloning vectors for high-throughput genetic 
            analysis in Saccharomyces cerevisiae.
  JOURNAL   Yeast. 2007 Oct . 24(10):913-9.
  PUBMED    17583893
REFERENCE   2  (bases 1 to 8069)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Yeast. 2007
            Oct . 24(10):913-9."
COMMENT     Sequence Label: pAG413GAL-ccdB-EGFP
FEATURES             Location/Qualifiers
     source          1..8069
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(10..28)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     misc_feature    70..573
                     /label=CEN/ARS
                     /note="S. cerevisiae CEN6 centromere fused to an 
                     autonomously replicating sequence"
     promoter        603..707
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(708..776,777..1568)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(926..945)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      1739..2327
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2228..2247
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     2481..2498
                     /label=L4440
                     /note="L4440 vector, forward primer"
     promoter        join(2651..2656,2657..2674,2675..2681)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    2689..2705
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     2694..2716
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     2713..2729
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     2713..2729
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     2748..2768
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        2750..2768
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        2792..3233
                     /gene="S. cerevisiae GAL1"
                     /label=GAL1 promoter
                     /note="inducible promoter, regulated by Gal4"
     protein_bind    2792..2909
                     /label=UAS
                     /bound_moiety="Gal4"
                     /note="upstream activating sequence mediating 
                     Gal4-dependent induction"
     primer_bind     3204..3227
                     /label=GAL1
                     /note="S. cerevisiae GAL1 promoter, forward primer"
     primer_bind     3248..3264
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     3248..3264
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     protein_bind    3272..3396
                     /gene="mutant version of attR"
                     /label=attR1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     promoter        join(3421..3426,3427..3444,3445..3451)
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an ""up"" mutation"
     CDS             3505..4164
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     complement(3571..3590)
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     CDS             4509..4814
                     /codon_start=1
                     /gene="ccdB"
                     /product="CcdB, a bacterial toxin that poisons DNA gyrase"
                     /label=ccdB
                     /note="Plasmids containing the ccdB gene cannot be 
                     propagated in standard E. coli strains."
                     /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK
                     VSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     primer_bind     4693..4712
                     /label=ccdB-fwd
                     /note="ccdB gene, forward primer"
     protein_bind    complement(4855..4979)
                     /gene="mutant version of attR"
                     /label=attR2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     CDS             join(5011..5013,5014..5016,5017..5730)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(5011..5013,5014..5016,5017..5727)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(5056..5077)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(5317..5336)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     5664..5685
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     terminator      5737..5984
                     /gene="S. cerevisiae CYC1"
                     /label=CYC1 terminator
                     /note="transcription terminator for CYC1"
     primer_bind     complement(5753..5771)
                     /label=CYC1
                     /note="CYC1 transcription termination signal, reverse 
                     primer"
     primer_bind     complement(6002..6021)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(6003..6021)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(6031..6048)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(6031..6047)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6040..6062)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      6192..6647
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(6279..6298)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     6489..6510
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     CDS             complement(6907..7566)
                     /codon_start=1
                     /gene="S. cerevisiae HIS3"
                     /product="imidazoleglycerol-phosphate dehydratase, required
                     for histidine biosynthesis"
                     /label=HIS3
                     /note="yeast auxotrophic marker"
                     /translation="MTEQKALVKRITNETKIQIAISLKGGPLAIEHSIFPEKEAEAVAE
                     QATQSQVINVHTGIGFLDHMIHALAKHSGWSLIVECIGDLHIDDHHTTEDCGIALGQAF
                     KEALLARGVKRFGSGFAPLDEALSRAVVDLSNRPYAVVELGLQREKVGDLSCEMIPHFL
                     ESFAEASRITLHVDCLRGKNDHHRSESAFKALAVAIREATSPNGTNDVPSTKGVLM"
     promoter        complement(7567..7753)
                     /gene="S. cerevisiae HIS3"
                     /label=HIS3 promoter
     primer_bind     complement(7900..7919)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     8019..8041
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
ORIGIN
        1 gacgaaaggg cctcgtgata cgcctatttt tataggttaa tgtcatgata ataatggttt
       61 cttaggacga tcacgtgcta taaaaataat tataatttaa attttttaat ataaatatat
      121 aaattaaaaa tagaaagtaa aaaaagaaat taaagaaaaa atagtttttg ttttccgaag
      181 atgtaaaaga ctctaggggg atcgccaaca aatactacct tttatcttgc tcttcctgct
      241 ctcaggtatt aatgccgaat tgtttcatct tgtctgtgta gaagaccaca cacgaaaatc
      301 ctgtgatttt acattttact tatcgttaat cgaatgtata tctatttaat ctgcttttct
      361 tgtctaataa atatatatgt aaagtacgct ttttgttgaa attttttaaa cctttgttta
      421 tttttttttc ttcattccgt aactcttcta ccttctttat ttactttcta aaatccaaat
      481 acaaaacata aaaataaata aacacagagt aaattcccaa attattccat cattaaaaga
      541 tacgaggcgc gtgtaagtta caggcaagcg atccgtcagg tggcactttt cggggaaatg
      601 tgcgcggaac ccctatttgt ttatttttct aaatacattc aaatatgtat ccgctcatga
      661 gacaataacc ctgataaatg cttcaataat attgaaaaag gaagagtatg agtattcaac
      721 atttccgtgt cgcccttatt cccttttttg cggcattttg ccttcctgtt tttgctcacc
      781 cagaaacgct ggtgaaagta aaagatgctg aagatcagtt gggtgcacga gtgggttaca
      841 tcgaactgga tctcaacagc ggtaagatcc ttgagagttt tcgccccgaa gaacgttttc
      901 caatgatgag cacttttaaa gttctgctat gtggcgcggt attatcccgt attgacgccg
      961 ggcaagagca actcggtcgc cgcatacact attctcagaa tgacttggtt gagtactcac
     1021 cagtcacaga aaagcatctt acggatggca tgacagtaag agaattatgc agtgctgcca
     1081 taaccatgag tgataacact gcggccaact tacttctgac aacgatcgga ggaccgaagg
     1141 agctaaccgc ttttttgcac aacatggggg atcatgtaac tcgccttgat cgttgggaac
     1201 cggagctgaa tgaagccata ccaaacgacg agcgtgacac cacgatgcct gtagcaatgg
     1261 caacaacgtt gcgcaaacta ttaactggcg aactacttac tctagcttcc cggcaacaat
     1321 taatagactg gatggaggcg gataaagttg caggaccact tctgcgctcg gcccttccgg
     1381 ctggctggtt tattgctgat aaatctggag ccggtgagcg tgggtctcgc ggtatcattg
     1441 cagcactggg gccagatggt aagccctccc gtatcgtagt tatctacacg acggggagtc
     1501 aggcaactat ggatgaacga aatagacaga tcgctgagat aggtgcctca ctgattaagc
     1561 attggtaact gtcagaccaa gtttactcat atatacttta gattgattta aaacttcatt
     1621 tttaatttaa aaggatctag gtgaagatcc tttttgataa tctcatgacc aaaatccctt
     1681 aacgtgagtt ttcgttccac tgagcgtcag accccgtaga aaagatcaaa ggatcttctt
     1741 gagatccttt ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag
     1801 cggtggtttg tttgccggat caagagctac caactctttt tccgaaggta actggcttca
     1861 gcagagcgca gataccaaat actgtccttc tagtgtagcc gtagttaggc caccacttca
     1921 agaactctgt agcaccgcct acatacctcg ctctgctaat cctgttacca gtggctgctg
     1981 ccagtggcga taagtcgtgt cttaccgggt tggactcaag acgatagtta ccggataagg
     2041 cgcagcggtc gggctgaacg gggggttcgt gcacacagcc cagcttggag cgaacgacct
     2101 acaccgaact gagataccta cagcgtgagc tatgagaaag cgccacgctt cccgaaggga
     2161 gaaaggcgga caggtatccg gtaagcggca gggtcggaac aggagagcgc acgagggagc
     2221 ttccaggggg aaacgcctgg tatctttata gtcctgtcgg gtttcgccac ctctgacttg
     2281 agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac gccagcaacg
     2341 cggccttttt acggttcctg gccttttgct ggccttttgc tcacatgttc tttcctgcgt
     2401 tatcccctga ttctgtggat aaccgtatta ccgcctttga gtgagctgat accgctcgcc
     2461 gcagccgaac gaccgagcgc agcgagtcag tgagcgagga agcggaagag cgcccaatac
     2521 gcaaaccgcc tctccccgcg cgttggccga ttcattaatg cagctggcac gacaggtttc
     2581 ccgactggaa agcgggcagt gagcgcaacg caattaatgt gagttacctc actcattagg
     2641 caccccaggc tttacacttt atgcttccgg ctcctatgtt gtgtggaatt gtgagcggat
     2701 aacaatttca cacaggaaac agctatgacc atgattacgc caagcgcgca attaaccctc
     2761 actaaaggga acaaaagctg gagctctagt acggattaga agccgccgag cgggcgacag
     2821 ccctccgacg gaagactctc ctccgtgcgt cctcgtcttc accggtcgcg ttcctgaaac
     2881 gcagatgtgc ctcgcgccgc actgctccga acaataaaga ttctacaata ctagctttta
     2941 tggttatgaa gaggaaaaat tggcagtaac ctggccccac aaaccttcaa attaacgaat
     3001 caaattaaca accataggat gataatgcga ttagtttttt agccttattt ctggggtaat
     3061 taatcagcga agcgatgatt tttgatctat taacagatat ataaatggaa aagctgcata
     3121 accactttaa ctaatacttt caacattttc agtttgtatt acttcttatt caaatgtcat
     3181 aaaagtatca acaaaaaatt gttaatatac ctctatactt taacgtcaag gagaaaaaac
     3241 cccggattct agaactagtg gatcccccat cacaagtttg tacaaaaaag ctgaacgaga
     3301 aacgtaaaat gatataaata tcaatatatt aaattagatt ttgcataaaa aacagactac
     3361 ataatactgt aaaacacaac atatccagtc actatggcgg ccgcattagg caccccaggc
     3421 tttacacttt atgcttccgg ctcgtataat gtgtggattt tgagttagga tccgtcgaga
     3481 ttttcaggag ctaaggaagc taaaatggag aaaaaaatca ctggatatac caccgttgat
     3541 atatcccaat ggcatcgtaa agaacatttt gaggcatttc agtcagttgc tcaatgtacc
     3601 tataaccaga ccgttcagct ggatattacg gcctttttaa agaccgtaaa gaaaaataag
     3661 cacaagtttt atccggcctt tattcacatt cttgcccgcc tgatgaatgc tcatccggaa
     3721 ttccgtatgg caatgaaaga cggtgagctg gtgatatggg atagtgttca cccttgttac
     3781 accgttttcc atgagcaaac tgaaacgttt tcatcgctct ggagtgaata ccacgacgat
     3841 ttccggcagt ttctacacat atattcgcaa gatgtggcgt gttacggtga aaacctggcc
     3901 tatttcccta aagggtttat tgagaatatg tttttcgtct cagccaatcc ctgggtgagt
     3961 ttcaccagtt ttgatttaaa cgtggccaat atggacaact tcttcgcccc cgttttcacc
     4021 atgggcaaat attatacgca aggcgacaag gtgctgatgc cgctggcgat tcaggttcat
     4081 catgccgtct gtgatggctt ccatgtcggc agaatgctta atgaattaca acagtactgc
     4141 gatgagtggc agggcggggc gtaaacgccg cgtggatccg gcttactaaa agccagataa
     4201 cagtatgcgt atttgcgcgc tgatttttgc ggtataagaa tatatactga tatgtatacc
     4261 cgaagtatgt caaaaagagg tatgctatga agcagcgtat tacagtgaca gttgacagcg
     4321 acagctatca gttgctcaag gcatatatga tgtcaatatc tccggtctgg taagcacaac
     4381 catgcagaat gaagcccgtc gtctgcgtgc cgaacgctgg aaagcggaaa atcaggaagg
     4441 gatggctgag gtcgcccggt ttattgaaat gaacggctct tttgctgacg agaacagggg
     4501 ctggtgaaat gcagtttaag gtttacacct ataaaagaga gagccgttat cgtctgtttg
     4561 tggatgtaca gagtgatatt attgacacgc ccgggcgacg gatggtgatc cccctggcca
     4621 gtgcacgtct gctgtcagat aaagtctccc gtgaacttta cccggtggtg catatcgggg
     4681 atgaaagctg gcgcatgatg accaccgata tggccagtgt gccggtctcc gttatcgggg
     4741 aagaagtggc tgatctcagc caccgcgaaa atgacatcaa aaacgccatt aacctgatgt
     4801 tctggggaat ataaatgtca ggctccctta tacacagcca gtctgcaggt cgaccatagt
     4861 gactggatat gttgtgtttt acagtattat gtagtctgtt ttttatgcaa aatctaattt
     4921 aatatattga tatttatatc attttacgtt tctcgttcag ctttcttgta caaagtggtg
     4981 atgggctgca ggaattcgat atcaagctta atggtgagca agggcgagga gctgttcacc
     5041 ggggtggtgc ccatcctggt cgagctggac ggcgacgtaa acggccacaa gttcagcgtg
     5101 tccggcgagg gcgagggcga tgccacctac ggcaagctga ccctgaagtt catctgcacc
     5161 accggcaagc tgcccgtgcc ctggcccacc ctcgtgacca ccctgaccta cggcgtgcag
     5221 tgcttcagcc gctaccccga ccacatgaag cagcacgact tcttcaagtc cgccatgccc
     5281 gaaggctacg tccaggagcg caccatcttc ttcaaggacg acggcaacta caagacccgc
     5341 gccgaggtga agttcgaggg cgacaccctg gtgaaccgca tcgagctgaa gggcatcgac
     5401 ttcaaggagg acggcaacat cctggggcac aagctggagt acaactacaa cagccacaac
     5461 gtctatatca tggccgacaa gcagaagaac ggcatcaagg tgaacttcaa gatccgccac
     5521 aacatcgagg acggcagcgt gcagctcgcc gaccactacc agcagaacac ccccatcggc
     5581 gacggccccg tgctgctgcc cgacaaccac tacctgagca cccagtccgc cctgagcaaa
     5641 gaccccaacg agaagcgcga tcacatggtc ctgctggagt tcgtgaccgc cgccgggatc
     5701 actctcggca tggacgagct gtacaagtaa ctcgagtcat gtaattagtt atgtcacgct
     5761 tacattcacg ccctcccccc acatccgctc taaccgaaaa ggaaggagtt agacaacctg
     5821 aagtctaggt ccctatttat ttttttatag ttatgttagt attaagaacg ttatttatat
     5881 ttcaaatttt tctttttttt ctgtacagac gcgtgtacgc atgtaacatt atactgaaaa
     5941 ccttgcttga gaaggttttg ggacgctcga aggctttaat ttgcggccgg tacccaattc
     6001 gccctatagt gagtcgtatt acgcgcgctc actggccgtc gttttacaac gtcgtgactg
     6061 ggaaaaccct ggcgttaccc aacttaatcg ccttgcagca catccccctt tcgccagctg
     6121 gcgtaatagc gaagaggccc gcaccgatcg cccttcccaa cagttgcgca gcctgaatgg
     6181 cgaatggcgc gacgcgccct gtagcggcgc attaagcgcg gcgggtgtgg tggttacgcg
     6241 cagcgtgacc gctacacttg ccagcgccct agcgcccgct cctttcgctt tcttcccttc
     6301 ctttctcgcc acgttcgccg gctttccccg tcaagctcta aatcgggggc tccctttagg
     6361 gttccgattt agtgctttac ggcacctcga ccccaaaaaa cttgattagg gtgatggttc
     6421 acgtagtggg ccatcgccct gatagacggt ttttcgccct ttgacgttgg agtccacgtt
     6481 ctttaatagt ggactcttgt tccaaactgg aacaacactc aaccctatct cggtctattc
     6541 ttttgattta taagggattt tgccgatttc ggcctattgg ttaaaaaatg agctgattta
     6601 acaaaaattt aacgcgaatt ttaacaaaat attaacgttt acaatttcct gatgcggtat
     6661 tttctcctta cgcatctgtg cggtatttca caccgcatag atccgtcgag ttcaagagaa
     6721 aaaaaaagaa aaagcaaaaa gaaaaaagga aagcgcgcct cgttcagaat gacacgtata
     6781 gaatgatgca ttaccttgtc atcttcagta tcatactgtt cgtatacata cttactgaca
     6841 ttcataggta tacatatata cacatgtata tatatcgtat gctgcagctt taaataatcg
     6901 gtgtcactac ataagaacac ctttggtgga gggaacatcg ttggtaccat tgggcgaggt
     6961 ggcttctctt atggcaaccg caagagcctt gaacgcactc tcactacggt gatgatcatt
     7021 cttgcctcgc agacaatcaa cgtggagggt aattctgcta gcctctgcaa agctttcaag
     7081 aaaatgcggg atcatctcgc aagagagatc tcctactttc tccctttgca aaccaagttc
     7141 gacaactgcg tacggcctgt tcgaaagatc taccaccgct ctggaaagtg cctcatccaa
     7201 aggcgcaaat cctgatccaa acctttttac tccacgcgcc agtagggcct ctttaaaagc
     7261 ttgaccgaga gcaatcccgc agtcttcagt ggtgtgatgg tcgtctatgt gtaagtcacc
     7321 aatgcactca acgattagcg accagccgga atgcttggcc agagcatgta tcatatggtc
     7381 cagaaaccct atacctgtgt ggacgttaat cacttgcgat tgtgtggcct gttctgctac
     7441 tgcttctgcc tctttttctg ggaagatcga gtgctctatc gctaggggac caccctttaa
     7501 agagatcgca atctgaatct tggtttcatt tgtaatacgc tttactaggg ctttctgctc
     7561 tgtcatcttt gccttcgttt atcttgcctg ctcatttttt agtatattct tcgaagaaat
     7621 cacattactt tatataatgt ataattcatt atgtgataat gccaatcgct aagaaaaaaa
     7681 aagagtcatc cgctagggga aaaaaaaaaa tgaaaatcat taccgaggca taaaaaaata
     7741 tagagtgtac tagaggaggc caagagtaat agaaaaagaa aattgcggga aaggactgtg
     7801 ttatgacttc cctgactaat gccgtgttca aacgatacct ggcagtgact cctagcgctc
     7861 accaagctct taaaacggga atttatggtg cactctcagt acaatctgct ctgatgccgc
     7921 atagttaagc cagccccgac acccgccaac acgcgctgac gcgccctgac gggcttgtct
     7981 gctcccggca tccgcttaca gacaagctgt gaccgtctcc gggagctgca tgtgtcagag
     8041 gttttcaccg tcatcaccga aacgcgcga
//
