LOCUS       sequence-127468-        8064 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8064)
  AUTHORS   Alberti S, Gitler AD, Lindquist S
  TITLE     A suite of Gateway cloning vectors for high-throughput genetic 
            analysis in Saccharomyces cerevisiae.
  JOURNAL   Yeast. 2007 Oct . 24(10):913-9.
  PUBMED    17583893
REFERENCE   2  (bases 1 to 8064)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Yeast. 2007
            Oct . 24(10):913-9."
COMMENT     Sequence Label: pAG413GAL-EGFP-ccdB
FEATURES             Location/Qualifiers
     source          1..8064
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(10..28)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     misc_feature    70..573
                     /label=CEN/ARS
                     /note="S. cerevisiae CEN6 centromere fused to an 
                     autonomously replicating sequence"
     promoter        603..707
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(708..776,777..1568)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(926..945)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      1739..2327
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2228..2247
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     2481..2498
                     /label=L4440
                     /note="L4440 vector, forward primer"
     promoter        join(2651..2656,2657..2674,2675..2681)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    2689..2705
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     2694..2716
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     2713..2729
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     2713..2729
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     2748..2768
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        2750..2768
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        2792..3233
                     /gene="S. cerevisiae GAL1"
                     /label=GAL1 promoter
                     /note="inducible promoter, regulated by Gal4"
     protein_bind    2792..2909
                     /label=UAS
                     /bound_moiety="Gal4"
                     /note="upstream activating sequence mediating 
                     Gal4-dependent induction"
     primer_bind     3204..3227
                     /label=GAL1
                     /note="S. cerevisiae GAL1 promoter, forward primer"
     primer_bind     3248..3264
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     3248..3264
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     CDS             join(3277..3279,3280..3282,3283..3993)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(3277..3279,3280..3282,3283..3993)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(3322..3343)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(3583..3602)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     3930..3951
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     protein_bind    4018..4142
                     /gene="mutant version of attR"
                     /label=attR1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     promoter        join(4167..4172,4173..4190,4191..4197)
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an ""up"" mutation"
     CDS             4251..4910
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     complement(4317..4336)
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     CDS             5255..5560
                     /codon_start=1
                     /gene="ccdB"
                     /product="CcdB, a bacterial toxin that poisons DNA gyrase"
                     /label=ccdB
                     /note="Plasmids containing the ccdB gene cannot be 
                     propagated in standard E. coli strains."
                     /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK
                     VSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     primer_bind     5439..5458
                     /label=ccdB-fwd
                     /note="ccdB gene, forward primer"
     protein_bind    complement(5601..5725)
                     /gene="mutant version of attR"
                     /label=attR2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     terminator      5732..5979
                     /gene="S. cerevisiae CYC1"
                     /label=CYC1 terminator
                     /note="transcription terminator for CYC1"
     primer_bind     complement(5748..5766)
                     /label=CYC1
                     /note="CYC1 transcription termination signal, reverse 
                     primer"
     primer_bind     complement(5997..6016)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(5998..6016)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(6026..6043)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(6026..6042)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6035..6057)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      6187..6642
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(6274..6293)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     6484..6505
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     CDS             complement(6902..7561)
                     /codon_start=1
                     /gene="S. cerevisiae HIS3"
                     /product="imidazoleglycerol-phosphate dehydratase, required
                     for histidine biosynthesis"
                     /label=HIS3
                     /note="yeast auxotrophic marker"
                     /translation="MTEQKALVKRITNETKIQIAISLKGGPLAIEHSIFPEKEAEAVAE
                     QATQSQVINVHTGIGFLDHMIHALAKHSGWSLIVECIGDLHIDDHHTTEDCGIALGQAF
                     KEALLARGVKRFGSGFAPLDEALSRAVVDLSNRPYAVVELGLQREKVGDLSCEMIPHFL
                     ESFAEASRITLHVDCLRGKNDHHRSESAFKALAVAIREATSPNGTNDVPSTKGVLM"
     promoter        complement(7562..7748)
                     /gene="S. cerevisiae HIS3"
                     /label=HIS3 promoter
     primer_bind     complement(7895..7914)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     8014..8036
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
ORIGIN
        1 gacgaaaggg cctcgtgata cgcctatttt tataggttaa tgtcatgata ataatggttt
       61 cttaggacga tcacgtgcta taaaaataat tataatttaa attttttaat ataaatatat
      121 aaattaaaaa tagaaagtaa aaaaagaaat taaagaaaaa atagtttttg ttttccgaag
      181 atgtaaaaga ctctaggggg atcgccaaca aatactacct tttatcttgc tcttcctgct
      241 ctcaggtatt aatgccgaat tgtttcatct tgtctgtgta gaagaccaca cacgaaaatc
      301 ctgtgatttt acattttact tatcgttaat cgaatgtata tctatttaat ctgcttttct
      361 tgtctaataa atatatatgt aaagtacgct ttttgttgaa attttttaaa cctttgttta
      421 tttttttttc ttcattccgt aactcttcta ccttctttat ttactttcta aaatccaaat
      481 acaaaacata aaaataaata aacacagagt aaattcccaa attattccat cattaaaaga
      541 tacgaggcgc gtgtaagtta caggcaagcg atccgtcagg tggcactttt cggggaaatg
      601 tgcgcggaac ccctatttgt ttatttttct aaatacattc aaatatgtat ccgctcatga
      661 gacaataacc ctgataaatg cttcaataat attgaaaaag gaagagtatg agtattcaac
      721 atttccgtgt cgcccttatt cccttttttg cggcattttg ccttcctgtt tttgctcacc
      781 cagaaacgct ggtgaaagta aaagatgctg aagatcagtt gggtgcacga gtgggttaca
      841 tcgaactgga tctcaacagc ggtaagatcc ttgagagttt tcgccccgaa gaacgttttc
      901 caatgatgag cacttttaaa gttctgctat gtggcgcggt attatcccgt attgacgccg
      961 ggcaagagca actcggtcgc cgcatacact attctcagaa tgacttggtt gagtactcac
     1021 cagtcacaga aaagcatctt acggatggca tgacagtaag agaattatgc agtgctgcca
     1081 taaccatgag tgataacact gcggccaact tacttctgac aacgatcgga ggaccgaagg
     1141 agctaaccgc ttttttgcac aacatggggg atcatgtaac tcgccttgat cgttgggaac
     1201 cggagctgaa tgaagccata ccaaacgacg agcgtgacac cacgatgcct gtagcaatgg
     1261 caacaacgtt gcgcaaacta ttaactggcg aactacttac tctagcttcc cggcaacaat
     1321 taatagactg gatggaggcg gataaagttg caggaccact tctgcgctcg gcccttccgg
     1381 ctggctggtt tattgctgat aaatctggag ccggtgagcg tgggtctcgc ggtatcattg
     1441 cagcactggg gccagatggt aagccctccc gtatcgtagt tatctacacg acggggagtc
     1501 aggcaactat ggatgaacga aatagacaga tcgctgagat aggtgcctca ctgattaagc
     1561 attggtaact gtcagaccaa gtttactcat atatacttta gattgattta aaacttcatt
     1621 tttaatttaa aaggatctag gtgaagatcc tttttgataa tctcatgacc aaaatccctt
     1681 aacgtgagtt ttcgttccac tgagcgtcag accccgtaga aaagatcaaa ggatcttctt
     1741 gagatccttt ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag
     1801 cggtggtttg tttgccggat caagagctac caactctttt tccgaaggta actggcttca
     1861 gcagagcgca gataccaaat actgtccttc tagtgtagcc gtagttaggc caccacttca
     1921 agaactctgt agcaccgcct acatacctcg ctctgctaat cctgttacca gtggctgctg
     1981 ccagtggcga taagtcgtgt cttaccgggt tggactcaag acgatagtta ccggataagg
     2041 cgcagcggtc gggctgaacg gggggttcgt gcacacagcc cagcttggag cgaacgacct
     2101 acaccgaact gagataccta cagcgtgagc tatgagaaag cgccacgctt cccgaaggga
     2161 gaaaggcgga caggtatccg gtaagcggca gggtcggaac aggagagcgc acgagggagc
     2221 ttccaggggg aaacgcctgg tatctttata gtcctgtcgg gtttcgccac ctctgacttg
     2281 agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac gccagcaacg
     2341 cggccttttt acggttcctg gccttttgct ggccttttgc tcacatgttc tttcctgcgt
     2401 tatcccctga ttctgtggat aaccgtatta ccgcctttga gtgagctgat accgctcgcc
     2461 gcagccgaac gaccgagcgc agcgagtcag tgagcgagga agcggaagag cgcccaatac
     2521 gcaaaccgcc tctccccgcg cgttggccga ttcattaatg cagctggcac gacaggtttc
     2581 ccgactggaa agcgggcagt gagcgcaacg caattaatgt gagttacctc actcattagg
     2641 caccccaggc tttacacttt atgcttccgg ctcctatgtt gtgtggaatt gtgagcggat
     2701 aacaatttca cacaggaaac agctatgacc atgattacgc caagcgcgca attaaccctc
     2761 actaaaggga acaaaagctg gagctctagt acggattaga agccgccgag cgggcgacag
     2821 ccctccgacg gaagactctc ctccgtgcgt cctcgtcttc accggtcgcg ttcctgaaac
     2881 gcagatgtgc ctcgcgccgc actgctccga acaataaaga ttctacaata ctagctttta
     2941 tggttatgaa gaggaaaaat tggcagtaac ctggccccac aaaccttcaa attaacgaat
     3001 caaattaaca accataggat gataatgcga ttagtttttt agccttattt ctggggtaat
     3061 taatcagcga agcgatgatt tttgatctat taacagatat ataaatggaa aagctgcata
     3121 accactttaa ctaatacttt caacattttc agtttgtatt acttcttatt caaatgtcat
     3181 aaaagtatca acaaaaaatt gttaatatac ctctatactt taacgtcaag gagaaaaaac
     3241 cccggattct agaactagtg gatcccccgg gacaaaatgg tgagcaaggg cgaggagctg
     3301 ttcaccgggg tggtgcccat cctggtcgag ctggacggcg acgtaaacgg ccacaagttc
     3361 agcgtgtccg gcgagggcga gggcgatgcc acctacggca agctgaccct gaagttcatc
     3421 tgcaccaccg gcaagctgcc cgtgccctgg cccaccctcg tgaccaccct gacctacggc
     3481 gtgcagtgct tcagccgcta ccccgaccac atgaagcagc acgacttctt caagtccgcc
     3541 atgcccgaag gctacgtcca ggagcgcacc atcttcttca aggacgacgg caactacaag
     3601 acccgcgccg aggtgaagtt cgagggcgac accctggtga accgcatcga gctgaagggc
     3661 atcgacttca aggaggacgg caacatcctg gggcacaagc tggagtacaa ctacaacagc
     3721 cacaacgtct atatcatggc cgacaagcag aagaacggca tcaaggtgaa cttcaagatc
     3781 cgccacaaca tcgaggacgg cagcgtgcag ctcgccgacc actaccagca gaacaccccc
     3841 atcggcgacg gccccgtgct gctgcccgac aaccactacc tgagcaccca gtccgccctg
     3901 agcaaagacc ccaacgagaa gcgcgatcac atggtcctgc tggagttcgt gaccgccgcc
     3961 gggatcactc tcggcatgga cgagctgtac aagggtggac caggtggtgg acatcaaaca
     4021 agtttgtaca aaaaagctga acgagaaacg taaaatgata taaatatcaa tatattaaat
     4081 tagattttgc ataaaaaaca gactacataa tactgtaaaa cacaacatat ccagtcacta
     4141 tggcggccgc attaggcacc ccaggcttta cactttatgc ttccggctcg tataatgtgt
     4201 ggattttgag ttaggatccg tcgagatttt caggagctaa ggaagctaaa atggagaaaa
     4261 aaatcactgg atataccacc gttgatatat cccaatggca tcgtaaagaa cattttgagg
     4321 catttcagtc agttgctcaa tgtacctata accagaccgt tcagctggat attacggcct
     4381 ttttaaagac cgtaaagaaa aataagcaca agttttatcc ggcctttatt cacattcttg
     4441 cccgcctgat gaatgctcat ccggaattcc gtatggcaat gaaagacggt gagctggtga
     4501 tatgggatag tgttcaccct tgttacaccg ttttccatga gcaaactgaa acgttttcat
     4561 cgctctggag tgaataccac gacgatttcc ggcagtttct acacatatat tcgcaagatg
     4621 tggcgtgtta cggtgaaaac ctggcctatt tccctaaagg gtttattgag aatatgtttt
     4681 tcgtctcagc caatccctgg gtgagtttca ccagttttga tttaaacgtg gccaatatgg
     4741 acaacttctt cgcccccgtt ttcaccatgg gcaaatatta tacgcaaggc gacaaggtgc
     4801 tgatgccgct ggcgattcag gttcatcatg ccgtctgtga tggcttccat gtcggcagaa
     4861 tgcttaatga attacaacag tactgcgatg agtggcaggg cggggcgtaa acgccgcgtg
     4921 gatccggctt actaaaagcc agataacagt atgcgtattt gcgcgctgat ttttgcggta
     4981 taagaatata tactgatatg tatacccgaa gtatgtcaaa aagaggtatg ctatgaagca
     5041 gcgtattaca gtgacagttg acagcgacag ctatcagttg ctcaaggcat atatgatgtc
     5101 aatatctccg gtctggtaag cacaaccatg cagaatgaag cccgtcgtct gcgtgccgaa
     5161 cgctggaaag cggaaaatca ggaagggatg gctgaggtcg cccggtttat tgaaatgaac
     5221 ggctcttttg ctgacgagaa caggggctgg tgaaatgcag tttaaggttt acacctataa
     5281 aagagagagc cgttatcgtc tgtttgtgga tgtacagagt gatattattg acacgcccgg
     5341 gcgacggatg gtgatccccc tggccagtgc acgtctgctg tcagataaag tctcccgtga
     5401 actttacccg gtggtgcata tcggggatga aagctggcgc atgatgacca ccgatatggc
     5461 cagtgtgccg gtctccgtta tcggggaaga agtggctgat ctcagccacc gcgaaaatga
     5521 catcaaaaac gccattaacc tgatgttctg gggaatataa atgtcaggct cccttataca
     5581 cagccagtct gcaggtcgac catagtgact ggatatgttg tgttttacag tattatgtag
     5641 tctgtttttt atgcaaaatc taatttaata tattgatatt tatatcattt tacgtttctc
     5701 gttcagcttt cttgtacaaa gtggttcgat gtcatgtaat tagttatgtc acgcttacat
     5761 tcacgccctc cccccacatc cgctctaacc gaaaaggaag gagttagaca acctgaagtc
     5821 taggtcccta tttatttttt tatagttatg ttagtattaa gaacgttatt tatatttcaa
     5881 atttttcttt tttttctgta cagacgcgtg tacgcatgta acattatact gaaaaccttg
     5941 cttgagaagg ttttgggacg ctcgaaggct ttaatttgcg gccggtaccc aattcgccct
     6001 atagtgagtc gtattacgcg cgctcactgg ccgtcgtttt acaacgtcgt gactgggaaa
     6061 accctggcgt tacccaactt aatcgccttg cagcacatcc ccctttcgcc agctggcgta
     6121 atagcgaaga ggcccgcacc gatcgccctt cccaacagtt gcgcagcctg aatggcgaat
     6181 ggcgcgacgc gccctgtagc ggcgcattaa gcgcggcggg tgtggtggtt acgcgcagcg
     6241 tgaccgctac acttgccagc gccctagcgc ccgctccttt cgctttcttc ccttcctttc
     6301 tcgccacgtt cgccggcttt ccccgtcaag ctctaaatcg ggggctccct ttagggttcc
     6361 gatttagtgc tttacggcac ctcgacccca aaaaacttga ttagggtgat ggttcacgta
     6421 gtgggccatc gccctgatag acggtttttc gccctttgac gttggagtcc acgttcttta
     6481 atagtggact cttgttccaa actggaacaa cactcaaccc tatctcggtc tattcttttg
     6541 atttataagg gattttgccg atttcggcct attggttaaa aaatgagctg atttaacaaa
     6601 aatttaacgc gaattttaac aaaatattaa cgtttacaat ttcctgatgc ggtattttct
     6661 ccttacgcat ctgtgcggta tttcacaccg catagatccg tcgagttcaa gagaaaaaaa
     6721 aagaaaaagc aaaaagaaaa aaggaaagcg cgcctcgttc agaatgacac gtatagaatg
     6781 atgcattacc ttgtcatctt cagtatcata ctgttcgtat acatacttac tgacattcat
     6841 aggtatacat atatacacat gtatatatat cgtatgctgc agctttaaat aatcggtgtc
     6901 actacataag aacacctttg gtggagggaa catcgttggt accattgggc gaggtggctt
     6961 ctcttatggc aaccgcaaga gccttgaacg cactctcact acggtgatga tcattcttgc
     7021 ctcgcagaca atcaacgtgg agggtaattc tgctagcctc tgcaaagctt tcaagaaaat
     7081 gcgggatcat ctcgcaagag agatctccta ctttctccct ttgcaaacca agttcgacaa
     7141 ctgcgtacgg cctgttcgaa agatctacca ccgctctgga aagtgcctca tccaaaggcg
     7201 caaatcctga tccaaacctt tttactccac gcgccagtag ggcctcttta aaagcttgac
     7261 cgagagcaat cccgcagtct tcagtggtgt gatggtcgtc tatgtgtaag tcaccaatgc
     7321 actcaacgat tagcgaccag ccggaatgct tggccagagc atgtatcata tggtccagaa
     7381 accctatacc tgtgtggacg ttaatcactt gcgattgtgt ggcctgttct gctactgctt
     7441 ctgcctcttt ttctgggaag atcgagtgct ctatcgctag gggaccaccc tttaaagaga
     7501 tcgcaatctg aatcttggtt tcatttgtaa tacgctttac tagggctttc tgctctgtca
     7561 tctttgcctt cgtttatctt gcctgctcat tttttagtat attcttcgaa gaaatcacat
     7621 tactttatat aatgtataat tcattatgtg ataatgccaa tcgctaagaa aaaaaaagag
     7681 tcatccgcta ggggaaaaaa aaaaatgaaa atcattaccg aggcataaaa aaatatagag
     7741 tgtactagag gaggccaaga gtaatagaaa aagaaaattg cgggaaagga ctgtgttatg
     7801 acttccctga ctaatgccgt gttcaaacga tacctggcag tgactcctag cgctcaccaa
     7861 gctcttaaaa cgggaattta tggtgcactc tcagtacaat ctgctctgat gccgcatagt
     7921 taagccagcc ccgacacccg ccaacacgcg ctgacgcgcc ctgacgggct tgtctgctcc
     7981 cggcatccgc ttacagacaa gctgtgaccg tctccgggag ctgcatgtgt cagaggtttt
     8041 caccgtcatc accgaaacgc gcga
//
