LOCUS       sequence-127469-        1088 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses rat Cx26 (Gjb2 CDS) with an 8 amino acid linker on the 
            N-terminus of the Connexin linking to Enhanced Blue Fluorescent 
            Protein 2 (FP fused to NT of connexin). CMV promoter..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1088)
  AUTHORS   Stout RF Jr, Snapp EL, Spray DC
  TITLE     Connexin Type and Fluorescent Protein-fusion Tag Determine 
            Structural Stability of Gap Junction Plaques.
  JOURNAL   J Biol Chem. 2015 Aug 11. pii: jbc.M115.659979.
  PUBMED    26265468
REFERENCE   2  (bases 1 to 1088)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Biol 
            Chem. 2015 Aug 11. pii: jbc.M115.659979."
COMMENT     Sequence Label: EBFP2-Cx26
FEATURES             Location/Qualifiers
     source          1..1088
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      46..55
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(52..54,55..57,58..768)
                     /codon_start=1
                     /product="enhanced blue variant of GFP (Ai et al., 2007)"
                     /label=EBFP2
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVRGEGEGDATNGKLTL
                     KFICTTGKLPVPWPTLVTTLSHGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GTYKTRAEVKFEGDTLVNRIELKGVDFKEDGNILGHKLEYNFNSHNIYIMAVKQKNGIK
                     VNFKIRHNVEDGSVQLADHYQQNTPIGDGPVLLPDSHYLSTQSVLSKDPNEKRDHMVLL
                     EFRTAAGITLGMDELYK"
     primer_bind     complement(97..118)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
ORIGIN
        1 cagagctggt ttagtgaacc gtcagatccg ctagcgctac cggtcgccac catggtgagc
       61 aagggcgagg agctgttcac cggggtggtg cccatcctgg tcgagctgga cggcgacgta
      121 aacggccaca agttcagcgt gaggggcgag ggcgagggcg atgccaccaa cggcaagctg
      181 accctgaagt tcatctgcac caccggcaag ctgcccgtgc cctggcccac cctcgtgacc
      241 accctgagcc acggcgtgca gtgcttcgcc cgctaccccg accacatgaa gcagcacgac
      301 ttcttcaagt ccgccatgcc cgaaggctac gtccaggagc gcaccatctt cttcaaggac
      361 gacggcacct acaagacccg cgccgaggtg aagttcgagg gcgacaccct ggtgaaccgc
      421 atcgagctga agggcgtcga cttcaaggag gacggcaaca tcctggggca caagctggag
      481 tacaacttca acagccacaa catctatatc atggccgtca agcagaagaa cggcatcaag
      541 gtgaacttca agatccgcca caacgtggag gacggcagcg tgcagctcgc cgaccactac
      601 cagcagaaca cccccatcgg cgacggcccc gtgctgctgc ccgacagcca ctacctgagc
      661 acccagtccg tgctgagcaa agaccccaac gagaagcgcg atcacatggt cctgctggag
      721 ttccgcaccg ccgccgggat cactctcggc atggacgagc tgtacaagtc cggactcaga
      781 tctcgagctc agatggattg gggcacacta cagagcatcc tcgggggtgt caacaagcac
      841 tccaccagca ttgggaaaat ctggctcact gtcctcttca tcttccgcat catgatcctc
      901 gtggtggccg cgaaggaggt gtggggagat gagcaagccg attttgtttg caacactctc
      961 cagcctggct gtaagaatgt gtgctacgac cactacttcc ccatctctca catccggctc
     1021 tgggctctgc agctgatcat ggtgtccacg ccggccctcc tggtagctat gcacgtggcc
     1081 taccggag
//
