LOCUS       sequence-127472-        8268 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8268)
  AUTHORS   Alberti S, Gitler AD, Lindquist S
  TITLE     A suite of Gateway cloning vectors for high-throughput genetic 
            analysis in Saccharomyces cerevisiae.
  JOURNAL   Yeast. 2007 Oct . 24(10):913-9.
  PUBMED    17583893
REFERENCE   2  (bases 1 to 8268)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Yeast. 2007
            Oct . 24(10):913-9."
COMMENT     Sequence Label: pAG413GPD-ccdB-EGFP
FEATURES             Location/Qualifiers
     source          1..8268
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(10..28)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     misc_feature    70..573
                     /label=CEN/ARS
                     /note="S. cerevisiae CEN6 centromere fused to an 
                     autonomously replicating sequence"
     promoter        603..707
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(708..776,777..1568)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(926..945)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      1739..2327
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2228..2247
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     2481..2498
                     /label=L4440
                     /note="L4440 vector, forward primer"
     promoter        join(2651..2656,2657..2674,2675..2681)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    2689..2705
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     2694..2716
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     2713..2729
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     2713..2729
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     2748..2768
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        2750..2768
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        2797..3441
                     /gene="S. cerevisiae TDH3"
                     /label=GAP promoter
                     /note="promoter for glyceraldehyde-3-phosphate 
                     dehydrogenase; also known as the TDH3 promoter"
     primer_bind     3335..3358
                     /label=GPDpro-F
                     /note="S. cerevisiae GPD promoter, forward primer"
     primer_bind     3447..3463
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     3447..3463
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     protein_bind    3471..3595
                     /gene="mutant version of attR"
                     /label=attR1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     promoter        join(3620..3625,3626..3643,3644..3650)
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an ""up"" mutation"
     CDS             3704..4363
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     complement(3770..3789)
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     CDS             4708..5013
                     /codon_start=1
                     /gene="ccdB"
                     /product="CcdB, a bacterial toxin that poisons DNA gyrase"
                     /label=ccdB
                     /note="Plasmids containing the ccdB gene cannot be 
                     propagated in standard E. coli strains."
                     /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK
                     VSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     primer_bind     4892..4911
                     /label=ccdB-fwd
                     /note="ccdB gene, forward primer"
     protein_bind    complement(5054..5178)
                     /gene="mutant version of attR"
                     /label=attR2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     CDS             join(5210..5212,5213..5215,5216..5929)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(5210..5212,5213..5215,5216..5926)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(5255..5276)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(5516..5535)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     5863..5884
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     terminator      5936..6183
                     /gene="S. cerevisiae CYC1"
                     /label=CYC1 terminator
                     /note="transcription terminator for CYC1"
     primer_bind     complement(5952..5970)
                     /label=CYC1
                     /note="CYC1 transcription termination signal, reverse 
                     primer"
     primer_bind     complement(6201..6220)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(6202..6220)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(6230..6247)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(6230..6246)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6239..6261)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      6391..6846
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(6478..6497)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     6688..6709
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     CDS             complement(7106..7765)
                     /codon_start=1
                     /gene="S. cerevisiae HIS3"
                     /product="imidazoleglycerol-phosphate dehydratase, required
                     for histidine biosynthesis"
                     /label=HIS3
                     /note="yeast auxotrophic marker"
                     /translation="MTEQKALVKRITNETKIQIAISLKGGPLAIEHSIFPEKEAEAVAE
                     QATQSQVINVHTGIGFLDHMIHALAKHSGWSLIVECIGDLHIDDHHTTEDCGIALGQAF
                     KEALLARGVKRFGSGFAPLDEALSRAVVDLSNRPYAVVELGLQREKVGDLSCEMIPHFL
                     ESFAEASRITLHVDCLRGKNDHHRSESAFKALAVAIREATSPNGTNDVPSTKGVLM"
     promoter        complement(7766..7952)
                     /gene="S. cerevisiae HIS3"
                     /label=HIS3 promoter
     primer_bind     complement(8099..8118)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     8218..8240
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
ORIGIN
        1 gacgaaaggg cctcgtgata cgcctatttt tataggttaa tgtcatgata ataatggttt
       61 cttaggacga tcacgtgcta taaaaataat tataatttaa attttttaat ataaatatat
      121 aaattaaaaa tagaaagtaa aaaaagaaat taaagaaaaa atagtttttg ttttccgaag
      181 atgtaaaaga ctctaggggg atcgccaaca aatactacct tttatcttgc tcttcctgct
      241 ctcaggtatt aatgccgaat tgtttcatct tgtctgtgta gaagaccaca cacgaaaatc
      301 ctgtgatttt acattttact tatcgttaat cgaatgtata tctatttaat ctgcttttct
      361 tgtctaataa atatatatgt aaagtacgct ttttgttgaa attttttaaa cctttgttta
      421 tttttttttc ttcattccgt aactcttcta ccttctttat ttactttcta aaatccaaat
      481 acaaaacata aaaataaata aacacagagt aaattcccaa attattccat cattaaaaga
      541 tacgaggcgc gtgtaagtta caggcaagcg atccgtcagg tggcactttt cggggaaatg
      601 tgcgcggaac ccctatttgt ttatttttct aaatacattc aaatatgtat ccgctcatga
      661 gacaataacc ctgataaatg cttcaataat attgaaaaag gaagagtatg agtattcaac
      721 atttccgtgt cgcccttatt cccttttttg cggcattttg ccttcctgtt tttgctcacc
      781 cagaaacgct ggtgaaagta aaagatgctg aagatcagtt gggtgcacga gtgggttaca
      841 tcgaactgga tctcaacagc ggtaagatcc ttgagagttt tcgccccgaa gaacgttttc
      901 caatgatgag cacttttaaa gttctgctat gtggcgcggt attatcccgt attgacgccg
      961 ggcaagagca actcggtcgc cgcatacact attctcagaa tgacttggtt gagtactcac
     1021 cagtcacaga aaagcatctt acggatggca tgacagtaag agaattatgc agtgctgcca
     1081 taaccatgag tgataacact gcggccaact tacttctgac aacgatcgga ggaccgaagg
     1141 agctaaccgc ttttttgcac aacatggggg atcatgtaac tcgccttgat cgttgggaac
     1201 cggagctgaa tgaagccata ccaaacgacg agcgtgacac cacgatgcct gtagcaatgg
     1261 caacaacgtt gcgcaaacta ttaactggcg aactacttac tctagcttcc cggcaacaat
     1321 taatagactg gatggaggcg gataaagttg caggaccact tctgcgctcg gcccttccgg
     1381 ctggctggtt tattgctgat aaatctggag ccggtgagcg tgggtctcgc ggtatcattg
     1441 cagcactggg gccagatggt aagccctccc gtatcgtagt tatctacacg acggggagtc
     1501 aggcaactat ggatgaacga aatagacaga tcgctgagat aggtgcctca ctgattaagc
     1561 attggtaact gtcagaccaa gtttactcat atatacttta gattgattta aaacttcatt
     1621 tttaatttaa aaggatctag gtgaagatcc tttttgataa tctcatgacc aaaatccctt
     1681 aacgtgagtt ttcgttccac tgagcgtcag accccgtaga aaagatcaaa ggatcttctt
     1741 gagatccttt ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag
     1801 cggtggtttg tttgccggat caagagctac caactctttt tccgaaggta actggcttca
     1861 gcagagcgca gataccaaat actgtccttc tagtgtagcc gtagttaggc caccacttca
     1921 agaactctgt agcaccgcct acatacctcg ctctgctaat cctgttacca gtggctgctg
     1981 ccagtggcga taagtcgtgt cttaccgggt tggactcaag acgatagtta ccggataagg
     2041 cgcagcggtc gggctgaacg gggggttcgt gcacacagcc cagcttggag cgaacgacct
     2101 acaccgaact gagataccta cagcgtgagc tatgagaaag cgccacgctt cccgaaggga
     2161 gaaaggcgga caggtatccg gtaagcggca gggtcggaac aggagagcgc acgagggagc
     2221 ttccaggggg aaacgcctgg tatctttata gtcctgtcgg gtttcgccac ctctgacttg
     2281 agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac gccagcaacg
     2341 cggccttttt acggttcctg gccttttgct ggccttttgc tcacatgttc tttcctgcgt
     2401 tatcccctga ttctgtggat aaccgtatta ccgcctttga gtgagctgat accgctcgcc
     2461 gcagccgaac gaccgagcgc agcgagtcag tgagcgagga agcggaagag cgcccaatac
     2521 gcaaaccgcc tctccccgcg cgttggccga ttcattaatg cagctggcac gacaggtttc
     2581 ccgactggaa agcgggcagt gagcgcaacg caattaatgt gagttacctc actcattagg
     2641 caccccaggc tttacacttt atgcttccgg ctcctatgtt gtgtggaatt gtgagcggat
     2701 aacaatttca cacaggaaac agctatgacc atgattacgc caagcgcgca attaaccctc
     2761 actaaaggga acaaaagctg gagctcttcg agtttatcat tatcaatact cgccatttca
     2821 aagaatacgt aaataattaa tagtagtgat tttcctaact ttatttagtc aaaaaattag
     2881 ccttttaatt ctgctgtaac ccgtacatgc ccaaaatagg gggcgggtta cacagaatat
     2941 ataacaccga aggtgcctgg gtgaacaggt ttattcctgg catccactaa atataatgga
     3001 gcccgctttt taagctggca tccagaaaaa aaaagaatcc cagcaccaaa atattgtttt
     3061 cttcaccaac catcagttca taggtccatt ctcttagcgc aactacagag aacaggggca
     3121 caaacaggca aaaaacgggc acaacctcaa tggagtgatg caacctgcct ggagtaaatg
     3181 atgacacaag gcaattgacc cacgcatgta tctatctcat tttcttacac cttctattac
     3241 cttctgctct ctctgatttg gaaaaagctg aaaaaaaagg ttgaaaccag ttccctgaaa
     3301 ttattcccct acttgactaa taagtatata aagacggtag gtattgattg taattctgta
     3361 aatctatttc ttaaacttct taaattctac ttttatagtt agtctttttt ttagttttaa
     3421 aacaccagaa cttagtttcg acggattcta gaactagtgg atcccccatc acaagtttgt
     3481 acaaaaaagc tgaacgagaa acgtaaaatg atataaatat caatatatta aattagattt
     3541 tgcataaaaa acagactaca taatactgta aaacacaaca tatccagtca ctatggcggc
     3601 cgcattaggc accccaggct ttacacttta tgcttccggc tcgtataatg tgtggatttt
     3661 gagttaggat ccgtcgagat tttcaggagc taaggaagct aaaatggaga aaaaaatcac
     3721 tggatatacc accgttgata tatcccaatg gcatcgtaaa gaacattttg aggcatttca
     3781 gtcagttgct caatgtacct ataaccagac cgttcagctg gatattacgg cctttttaaa
     3841 gaccgtaaag aaaaataagc acaagtttta tccggccttt attcacattc ttgcccgcct
     3901 gatgaatgct catccggaat tccgtatggc aatgaaagac ggtgagctgg tgatatggga
     3961 tagtgttcac ccttgttaca ccgttttcca tgagcaaact gaaacgtttt catcgctctg
     4021 gagtgaatac cacgacgatt tccggcagtt tctacacata tattcgcaag atgtggcgtg
     4081 ttacggtgaa aacctggcct atttccctaa agggtttatt gagaatatgt ttttcgtctc
     4141 agccaatccc tgggtgagtt tcaccagttt tgatttaaac gtggccaata tggacaactt
     4201 cttcgccccc gttttcacca tgggcaaata ttatacgcaa ggcgacaagg tgctgatgcc
     4261 gctggcgatt caggttcatc atgccgtctg tgatggcttc catgtcggca gaatgcttaa
     4321 tgaattacaa cagtactgcg atgagtggca gggcggggcg taaacgccgc gtggatccgg
     4381 cttactaaaa gccagataac agtatgcgta tttgcgcgct gatttttgcg gtataagaat
     4441 atatactgat atgtataccc gaagtatgtc aaaaagaggt atgctatgaa gcagcgtatt
     4501 acagtgacag ttgacagcga cagctatcag ttgctcaagg catatatgat gtcaatatct
     4561 ccggtctggt aagcacaacc atgcagaatg aagcccgtcg tctgcgtgcc gaacgctgga
     4621 aagcggaaaa tcaggaaggg atggctgagg tcgcccggtt tattgaaatg aacggctctt
     4681 ttgctgacga gaacaggggc tggtgaaatg cagtttaagg tttacaccta taaaagagag
     4741 agccgttatc gtctgtttgt ggatgtacag agtgatatta ttgacacgcc cgggcgacgg
     4801 atggtgatcc ccctggccag tgcacgtctg ctgtcagata aagtctcccg tgaactttac
     4861 ccggtggtgc atatcgggga tgaaagctgg cgcatgatga ccaccgatat ggccagtgtg
     4921 ccggtctccg ttatcgggga agaagtggct gatctcagcc accgcgaaaa tgacatcaaa
     4981 aacgccatta acctgatgtt ctggggaata taaatgtcag gctcccttat acacagccag
     5041 tctgcaggtc gaccatagtg actggatatg ttgtgtttta cagtattatg tagtctgttt
     5101 tttatgcaaa atctaattta atatattgat atttatatca ttttacgttt ctcgttcagc
     5161 tttcttgtac aaagtggtga tgggctgcag gaattcgata tcaagcttaa tggtgagcaa
     5221 gggcgaggag ctgttcaccg gggtggtgcc catcctggtc gagctggacg gcgacgtaaa
     5281 cggccacaag ttcagcgtgt ccggcgaggg cgagggcgat gccacctacg gcaagctgac
     5341 cctgaagttc atctgcacca ccggcaagct gcccgtgccc tggcccaccc tcgtgaccac
     5401 cctgacctac ggcgtgcagt gcttcagccg ctaccccgac cacatgaagc agcacgactt
     5461 cttcaagtcc gccatgcccg aaggctacgt ccaggagcgc accatcttct tcaaggacga
     5521 cggcaactac aagacccgcg ccgaggtgaa gttcgagggc gacaccctgg tgaaccgcat
     5581 cgagctgaag ggcatcgact tcaaggagga cggcaacatc ctggggcaca agctggagta
     5641 caactacaac agccacaacg tctatatcat ggccgacaag cagaagaacg gcatcaaggt
     5701 gaacttcaag atccgccaca acatcgagga cggcagcgtg cagctcgccg accactacca
     5761 gcagaacacc cccatcggcg acggccccgt gctgctgccc gacaaccact acctgagcac
     5821 ccagtccgcc ctgagcaaag accccaacga gaagcgcgat cacatggtcc tgctggagtt
     5881 cgtgaccgcc gccgggatca ctctcggcat ggacgagctg tacaagtaac tcgagtcatg
     5941 taattagtta tgtcacgctt acattcacgc cctcccccca catccgctct aaccgaaaag
     6001 gaaggagtta gacaacctga agtctaggtc cctatttatt tttttatagt tatgttagta
     6061 ttaagaacgt tatttatatt tcaaattttt cttttttttc tgtacagacg cgtgtacgca
     6121 tgtaacatta tactgaaaac cttgcttgag aaggttttgg gacgctcgaa ggctttaatt
     6181 tgcggccggt acccaattcg ccctatagtg agtcgtatta cgcgcgctca ctggccgtcg
     6241 ttttacaacg tcgtgactgg gaaaaccctg gcgttaccca acttaatcgc cttgcagcac
     6301 atcccccttt cgccagctgg cgtaatagcg aagaggcccg caccgatcgc ccttcccaac
     6361 agttgcgcag cctgaatggc gaatggcgcg acgcgccctg tagcggcgca ttaagcgcgg
     6421 cgggtgtggt ggttacgcgc agcgtgaccg ctacacttgc cagcgcccta gcgcccgctc
     6481 ctttcgcttt cttcccttcc tttctcgcca cgttcgccgg ctttccccgt caagctctaa
     6541 atcgggggct ccctttaggg ttccgattta gtgctttacg gcacctcgac cccaaaaaac
     6601 ttgattaggg tgatggttca cgtagtgggc catcgccctg atagacggtt tttcgccctt
     6661 tgacgttgga gtccacgttc tttaatagtg gactcttgtt ccaaactgga acaacactca
     6721 accctatctc ggtctattct tttgatttat aagggatttt gccgatttcg gcctattggt
     6781 taaaaaatga gctgatttaa caaaaattta acgcgaattt taacaaaata ttaacgttta
     6841 caatttcctg atgcggtatt ttctccttac gcatctgtgc ggtatttcac accgcataga
     6901 tccgtcgagt tcaagagaaa aaaaaagaaa aagcaaaaag aaaaaaggaa agcgcgcctc
     6961 gttcagaatg acacgtatag aatgatgcat taccttgtca tcttcagtat catactgttc
     7021 gtatacatac ttactgacat tcataggtat acatatatac acatgtatat atatcgtatg
     7081 ctgcagcttt aaataatcgg tgtcactaca taagaacacc tttggtggag ggaacatcgt
     7141 tggtaccatt gggcgaggtg gcttctctta tggcaaccgc aagagccttg aacgcactct
     7201 cactacggtg atgatcattc ttgcctcgca gacaatcaac gtggagggta attctgctag
     7261 cctctgcaaa gctttcaaga aaatgcggga tcatctcgca agagagatct cctactttct
     7321 ccctttgcaa accaagttcg acaactgcgt acggcctgtt cgaaagatct accaccgctc
     7381 tggaaagtgc ctcatccaaa ggcgcaaatc ctgatccaaa cctttttact ccacgcgcca
     7441 gtagggcctc tttaaaagct tgaccgagag caatcccgca gtcttcagtg gtgtgatggt
     7501 cgtctatgtg taagtcacca atgcactcaa cgattagcga ccagccggaa tgcttggcca
     7561 gagcatgtat catatggtcc agaaacccta tacctgtgtg gacgttaatc acttgcgatt
     7621 gtgtggcctg ttctgctact gcttctgcct ctttttctgg gaagatcgag tgctctatcg
     7681 ctaggggacc accctttaaa gagatcgcaa tctgaatctt ggtttcattt gtaatacgct
     7741 ttactagggc tttctgctct gtcatctttg ccttcgttta tcttgcctgc tcatttttta
     7801 gtatattctt cgaagaaatc acattacttt atataatgta taattcatta tgtgataatg
     7861 ccaatcgcta agaaaaaaaa agagtcatcc gctaggggaa aaaaaaaaat gaaaatcatt
     7921 accgaggcat aaaaaaatat agagtgtact agaggaggcc aagagtaata gaaaaagaaa
     7981 attgcgggaa aggactgtgt tatgacttcc ctgactaatg ccgtgttcaa acgatacctg
     8041 gcagtgactc ctagcgctca ccaagctctt aaaacgggaa tttatggtgc actctcagta
     8101 caatctgctc tgatgccgca tagttaagcc agccccgaca cccgccaaca cgcgctgacg
     8161 cgccctgacg ggcttgtctg ctcccggcat ccgcttacag acaagctgtg accgtctccg
     8221 ggagctgcat gtgtcagagg ttttcaccgt catcaccgaa acgcgcga
//
