LOCUS       sequence-127475-        8263 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8263)
  AUTHORS   Alberti S, Gitler AD, Lindquist S
  TITLE     A suite of Gateway cloning vectors for high-throughput genetic 
            analysis in Saccharomyces cerevisiae.
  JOURNAL   Yeast. 2007 Oct . 24(10):913-9.
  PUBMED    17583893
REFERENCE   2  (bases 1 to 8263)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Yeast. 2007
            Oct . 24(10):913-9."
COMMENT     Sequence Label: pAG413GPD-EGFP-ccdB
FEATURES             Location/Qualifiers
     source          1..8263
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(10..28)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     misc_feature    70..573
                     /label=CEN/ARS
                     /note="S. cerevisiae CEN6 centromere fused to an 
                     autonomously replicating sequence"
     promoter        603..707
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(708..776,777..1568)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(926..945)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      1739..2327
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2228..2247
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     2481..2498
                     /label=L4440
                     /note="L4440 vector, forward primer"
     promoter        join(2651..2656,2657..2674,2675..2681)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    2689..2705
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     2694..2716
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     2713..2729
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     2713..2729
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     2748..2768
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        2750..2768
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        2797..3441
                     /gene="S. cerevisiae TDH3"
                     /label=GAP promoter
                     /note="promoter for glyceraldehyde-3-phosphate 
                     dehydrogenase; also known as the TDH3 promoter"
     primer_bind     3335..3358
                     /label=GPDpro-F
                     /note="S. cerevisiae GPD promoter, forward primer"
     primer_bind     3447..3463
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     3447..3463
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     CDS             join(3476..3478,3479..3481,3482..4192)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(3476..3478,3479..3481,3482..4192)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(3521..3542)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(3782..3801)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     4129..4150
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     protein_bind    4217..4341
                     /gene="mutant version of attR"
                     /label=attR1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     promoter        join(4366..4371,4372..4389,4390..4396)
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an ""up"" mutation"
     CDS             4450..5109
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     complement(4516..4535)
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     CDS             5454..5759
                     /codon_start=1
                     /gene="ccdB"
                     /product="CcdB, a bacterial toxin that poisons DNA gyrase"
                     /label=ccdB
                     /note="Plasmids containing the ccdB gene cannot be 
                     propagated in standard E. coli strains."
                     /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK
                     VSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     primer_bind     5638..5657
                     /label=ccdB-fwd
                     /note="ccdB gene, forward primer"
     protein_bind    complement(5800..5924)
                     /gene="mutant version of attR"
                     /label=attR2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     terminator      5931..6178
                     /gene="S. cerevisiae CYC1"
                     /label=CYC1 terminator
                     /note="transcription terminator for CYC1"
     primer_bind     complement(5947..5965)
                     /label=CYC1
                     /note="CYC1 transcription termination signal, reverse 
                     primer"
     primer_bind     complement(6196..6215)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(6197..6215)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(6225..6242)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(6225..6241)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6234..6256)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      6386..6841
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(6473..6492)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     6683..6704
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     CDS             complement(7101..7760)
                     /codon_start=1
                     /gene="S. cerevisiae HIS3"
                     /product="imidazoleglycerol-phosphate dehydratase, required
                     for histidine biosynthesis"
                     /label=HIS3
                     /note="yeast auxotrophic marker"
                     /translation="MTEQKALVKRITNETKIQIAISLKGGPLAIEHSIFPEKEAEAVAE
                     QATQSQVINVHTGIGFLDHMIHALAKHSGWSLIVECIGDLHIDDHHTTEDCGIALGQAF
                     KEALLARGVKRFGSGFAPLDEALSRAVVDLSNRPYAVVELGLQREKVGDLSCEMIPHFL
                     ESFAEASRITLHVDCLRGKNDHHRSESAFKALAVAIREATSPNGTNDVPSTKGVLM"
     promoter        complement(7761..7947)
                     /gene="S. cerevisiae HIS3"
                     /label=HIS3 promoter
     primer_bind     complement(8094..8113)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     8213..8235
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
ORIGIN
        1 gacgaaaggg cctcgtgata cgcctatttt tataggttaa tgtcatgata ataatggttt
       61 cttaggacga tcacgtgcta taaaaataat tataatttaa attttttaat ataaatatat
      121 aaattaaaaa tagaaagtaa aaaaagaaat taaagaaaaa atagtttttg ttttccgaag
      181 atgtaaaaga ctctaggggg atcgccaaca aatactacct tttatcttgc tcttcctgct
      241 ctcaggtatt aatgccgaat tgtttcatct tgtctgtgta gaagaccaca cacgaaaatc
      301 ctgtgatttt acattttact tatcgttaat cgaatgtata tctatttaat ctgcttttct
      361 tgtctaataa atatatatgt aaagtacgct ttttgttgaa attttttaaa cctttgttta
      421 tttttttttc ttcattccgt aactcttcta ccttctttat ttactttcta aaatccaaat
      481 acaaaacata aaaataaata aacacagagt aaattcccaa attattccat cattaaaaga
      541 tacgaggcgc gtgtaagtta caggcaagcg atccgtcagg tggcactttt cggggaaatg
      601 tgcgcggaac ccctatttgt ttatttttct aaatacattc aaatatgtat ccgctcatga
      661 gacaataacc ctgataaatg cttcaataat attgaaaaag gaagagtatg agtattcaac
      721 atttccgtgt cgcccttatt cccttttttg cggcattttg ccttcctgtt tttgctcacc
      781 cagaaacgct ggtgaaagta aaagatgctg aagatcagtt gggtgcacga gtgggttaca
      841 tcgaactgga tctcaacagc ggtaagatcc ttgagagttt tcgccccgaa gaacgttttc
      901 caatgatgag cacttttaaa gttctgctat gtggcgcggt attatcccgt attgacgccg
      961 ggcaagagca actcggtcgc cgcatacact attctcagaa tgacttggtt gagtactcac
     1021 cagtcacaga aaagcatctt acggatggca tgacagtaag agaattatgc agtgctgcca
     1081 taaccatgag tgataacact gcggccaact tacttctgac aacgatcgga ggaccgaagg
     1141 agctaaccgc ttttttgcac aacatggggg atcatgtaac tcgccttgat cgttgggaac
     1201 cggagctgaa tgaagccata ccaaacgacg agcgtgacac cacgatgcct gtagcaatgg
     1261 caacaacgtt gcgcaaacta ttaactggcg aactacttac tctagcttcc cggcaacaat
     1321 taatagactg gatggaggcg gataaagttg caggaccact tctgcgctcg gcccttccgg
     1381 ctggctggtt tattgctgat aaatctggag ccggtgagcg tgggtctcgc ggtatcattg
     1441 cagcactggg gccagatggt aagccctccc gtatcgtagt tatctacacg acggggagtc
     1501 aggcaactat ggatgaacga aatagacaga tcgctgagat aggtgcctca ctgattaagc
     1561 attggtaact gtcagaccaa gtttactcat atatacttta gattgattta aaacttcatt
     1621 tttaatttaa aaggatctag gtgaagatcc tttttgataa tctcatgacc aaaatccctt
     1681 aacgtgagtt ttcgttccac tgagcgtcag accccgtaga aaagatcaaa ggatcttctt
     1741 gagatccttt ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag
     1801 cggtggtttg tttgccggat caagagctac caactctttt tccgaaggta actggcttca
     1861 gcagagcgca gataccaaat actgtccttc tagtgtagcc gtagttaggc caccacttca
     1921 agaactctgt agcaccgcct acatacctcg ctctgctaat cctgttacca gtggctgctg
     1981 ccagtggcga taagtcgtgt cttaccgggt tggactcaag acgatagtta ccggataagg
     2041 cgcagcggtc gggctgaacg gggggttcgt gcacacagcc cagcttggag cgaacgacct
     2101 acaccgaact gagataccta cagcgtgagc tatgagaaag cgccacgctt cccgaaggga
     2161 gaaaggcgga caggtatccg gtaagcggca gggtcggaac aggagagcgc acgagggagc
     2221 ttccaggggg aaacgcctgg tatctttata gtcctgtcgg gtttcgccac ctctgacttg
     2281 agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac gccagcaacg
     2341 cggccttttt acggttcctg gccttttgct ggccttttgc tcacatgttc tttcctgcgt
     2401 tatcccctga ttctgtggat aaccgtatta ccgcctttga gtgagctgat accgctcgcc
     2461 gcagccgaac gaccgagcgc agcgagtcag tgagcgagga agcggaagag cgcccaatac
     2521 gcaaaccgcc tctccccgcg cgttggccga ttcattaatg cagctggcac gacaggtttc
     2581 ccgactggaa agcgggcagt gagcgcaacg caattaatgt gagttacctc actcattagg
     2641 caccccaggc tttacacttt atgcttccgg ctcctatgtt gtgtggaatt gtgagcggat
     2701 aacaatttca cacaggaaac agctatgacc atgattacgc caagcgcgca attaaccctc
     2761 actaaaggga acaaaagctg gagctcttcg agtttatcat tatcaatact cgccatttca
     2821 aagaatacgt aaataattaa tagtagtgat tttcctaact ttatttagtc aaaaaattag
     2881 ccttttaatt ctgctgtaac ccgtacatgc ccaaaatagg gggcgggtta cacagaatat
     2941 ataacaccga aggtgcctgg gtgaacaggt ttattcctgg catccactaa atataatgga
     3001 gcccgctttt taagctggca tccagaaaaa aaaagaatcc cagcaccaaa atattgtttt
     3061 cttcaccaac catcagttca taggtccatt ctcttagcgc aactacagag aacaggggca
     3121 caaacaggca aaaaacgggc acaacctcaa tggagtgatg caacctgcct ggagtaaatg
     3181 atgacacaag gcaattgacc cacgcatgta tctatctcat tttcttacac cttctattac
     3241 cttctgctct ctctgatttg gaaaaagctg aaaaaaaagg ttgaaaccag ttccctgaaa
     3301 ttattcccct acttgactaa taagtatata aagacggtag gtattgattg taattctgta
     3361 aatctatttc ttaaacttct taaattctac ttttatagtt agtctttttt ttagttttaa
     3421 aacaccagaa cttagtttcg acggattcta gaactagtgg atcccccggg acaaaatggt
     3481 gagcaagggc gaggagctgt tcaccggggt ggtgcccatc ctggtcgagc tggacggcga
     3541 cgtaaacggc cacaagttca gcgtgtccgg cgagggcgag ggcgatgcca cctacggcaa
     3601 gctgaccctg aagttcatct gcaccaccgg caagctgccc gtgccctggc ccaccctcgt
     3661 gaccaccctg acctacggcg tgcagtgctt cagccgctac cccgaccaca tgaagcagca
     3721 cgacttcttc aagtccgcca tgcccgaagg ctacgtccag gagcgcacca tcttcttcaa
     3781 ggacgacggc aactacaaga cccgcgccga ggtgaagttc gagggcgaca ccctggtgaa
     3841 ccgcatcgag ctgaagggca tcgacttcaa ggaggacggc aacatcctgg ggcacaagct
     3901 ggagtacaac tacaacagcc acaacgtcta tatcatggcc gacaagcaga agaacggcat
     3961 caaggtgaac ttcaagatcc gccacaacat cgaggacggc agcgtgcagc tcgccgacca
     4021 ctaccagcag aacaccccca tcggcgacgg ccccgtgctg ctgcccgaca accactacct
     4081 gagcacccag tccgccctga gcaaagaccc caacgagaag cgcgatcaca tggtcctgct
     4141 ggagttcgtg accgccgccg ggatcactct cggcatggac gagctgtaca agggtggacc
     4201 aggtggtgga catcaaacaa gtttgtacaa aaaagctgaa cgagaaacgt aaaatgatat
     4261 aaatatcaat atattaaatt agattttgca taaaaaacag actacataat actgtaaaac
     4321 acaacatatc cagtcactat ggcggccgca ttaggcaccc caggctttac actttatgct
     4381 tccggctcgt ataatgtgtg gattttgagt taggatccgt cgagattttc aggagctaag
     4441 gaagctaaaa tggagaaaaa aatcactgga tataccaccg ttgatatatc ccaatggcat
     4501 cgtaaagaac attttgaggc atttcagtca gttgctcaat gtacctataa ccagaccgtt
     4561 cagctggata ttacggcctt tttaaagacc gtaaagaaaa ataagcacaa gttttatccg
     4621 gcctttattc acattcttgc ccgcctgatg aatgctcatc cggaattccg tatggcaatg
     4681 aaagacggtg agctggtgat atgggatagt gttcaccctt gttacaccgt tttccatgag
     4741 caaactgaaa cgttttcatc gctctggagt gaataccacg acgatttccg gcagtttcta
     4801 cacatatatt cgcaagatgt ggcgtgttac ggtgaaaacc tggcctattt ccctaaaggg
     4861 tttattgaga atatgttttt cgtctcagcc aatccctggg tgagtttcac cagttttgat
     4921 ttaaacgtgg ccaatatgga caacttcttc gcccccgttt tcaccatggg caaatattat
     4981 acgcaaggcg acaaggtgct gatgccgctg gcgattcagg ttcatcatgc cgtctgtgat
     5041 ggcttccatg tcggcagaat gcttaatgaa ttacaacagt actgcgatga gtggcagggc
     5101 ggggcgtaaa cgccgcgtgg atccggctta ctaaaagcca gataacagta tgcgtatttg
     5161 cgcgctgatt tttgcggtat aagaatatat actgatatgt atacccgaag tatgtcaaaa
     5221 agaggtatgc tatgaagcag cgtattacag tgacagttga cagcgacagc tatcagttgc
     5281 tcaaggcata tatgatgtca atatctccgg tctggtaagc acaaccatgc agaatgaagc
     5341 ccgtcgtctg cgtgccgaac gctggaaagc ggaaaatcag gaagggatgg ctgaggtcgc
     5401 ccggtttatt gaaatgaacg gctcttttgc tgacgagaac aggggctggt gaaatgcagt
     5461 ttaaggttta cacctataaa agagagagcc gttatcgtct gtttgtggat gtacagagtg
     5521 atattattga cacgcccggg cgacggatgg tgatccccct ggccagtgca cgtctgctgt
     5581 cagataaagt ctcccgtgaa ctttacccgg tggtgcatat cggggatgaa agctggcgca
     5641 tgatgaccac cgatatggcc agtgtgccgg tctccgttat cggggaagaa gtggctgatc
     5701 tcagccaccg cgaaaatgac atcaaaaacg ccattaacct gatgttctgg ggaatataaa
     5761 tgtcaggctc ccttatacac agccagtctg caggtcgacc atagtgactg gatatgttgt
     5821 gttttacagt attatgtagt ctgtttttta tgcaaaatct aatttaatat attgatattt
     5881 atatcatttt acgtttctcg ttcagctttc ttgtacaaag tggttcgatg tcatgtaatt
     5941 agttatgtca cgcttacatt cacgccctcc ccccacatcc gctctaaccg aaaaggaagg
     6001 agttagacaa cctgaagtct aggtccctat ttattttttt atagttatgt tagtattaag
     6061 aacgttattt atatttcaaa tttttctttt ttttctgtac agacgcgtgt acgcatgtaa
     6121 cattatactg aaaaccttgc ttgagaaggt tttgggacgc tcgaaggctt taatttgcgg
     6181 ccggtaccca attcgcccta tagtgagtcg tattacgcgc gctcactggc cgtcgtttta
     6241 caacgtcgtg actgggaaaa ccctggcgtt acccaactta atcgccttgc agcacatccc
     6301 cctttcgcca gctggcgtaa tagcgaagag gcccgcaccg atcgcccttc ccaacagttg
     6361 cgcagcctga atggcgaatg gcgcgacgcg ccctgtagcg gcgcattaag cgcggcgggt
     6421 gtggtggtta cgcgcagcgt gaccgctaca cttgccagcg ccctagcgcc cgctcctttc
     6481 gctttcttcc cttcctttct cgccacgttc gccggctttc cccgtcaagc tctaaatcgg
     6541 gggctccctt tagggttccg atttagtgct ttacggcacc tcgaccccaa aaaacttgat
     6601 tagggtgatg gttcacgtag tgggccatcg ccctgataga cggtttttcg ccctttgacg
     6661 ttggagtcca cgttctttaa tagtggactc ttgttccaaa ctggaacaac actcaaccct
     6721 atctcggtct attcttttga tttataaggg attttgccga tttcggccta ttggttaaaa
     6781 aatgagctga tttaacaaaa atttaacgcg aattttaaca aaatattaac gtttacaatt
     6841 tcctgatgcg gtattttctc cttacgcatc tgtgcggtat ttcacaccgc atagatccgt
     6901 cgagttcaag agaaaaaaaa agaaaaagca aaaagaaaaa aggaaagcgc gcctcgttca
     6961 gaatgacacg tatagaatga tgcattacct tgtcatcttc agtatcatac tgttcgtata
     7021 catacttact gacattcata ggtatacata tatacacatg tatatatatc gtatgctgca
     7081 gctttaaata atcggtgtca ctacataaga acacctttgg tggagggaac atcgttggta
     7141 ccattgggcg aggtggcttc tcttatggca accgcaagag ccttgaacgc actctcacta
     7201 cggtgatgat cattcttgcc tcgcagacaa tcaacgtgga gggtaattct gctagcctct
     7261 gcaaagcttt caagaaaatg cgggatcatc tcgcaagaga gatctcctac tttctccctt
     7321 tgcaaaccaa gttcgacaac tgcgtacggc ctgttcgaaa gatctaccac cgctctggaa
     7381 agtgcctcat ccaaaggcgc aaatcctgat ccaaaccttt ttactccacg cgccagtagg
     7441 gcctctttaa aagcttgacc gagagcaatc ccgcagtctt cagtggtgtg atggtcgtct
     7501 atgtgtaagt caccaatgca ctcaacgatt agcgaccagc cggaatgctt ggccagagca
     7561 tgtatcatat ggtccagaaa ccctatacct gtgtggacgt taatcacttg cgattgtgtg
     7621 gcctgttctg ctactgcttc tgcctctttt tctgggaaga tcgagtgctc tatcgctagg
     7681 ggaccaccct ttaaagagat cgcaatctga atcttggttt catttgtaat acgctttact
     7741 agggctttct gctctgtcat ctttgccttc gtttatcttg cctgctcatt ttttagtata
     7801 ttcttcgaag aaatcacatt actttatata atgtataatt cattatgtga taatgccaat
     7861 cgctaagaaa aaaaaagagt catccgctag gggaaaaaaa aaaatgaaaa tcattaccga
     7921 ggcataaaaa aatatagagt gtactagagg aggccaagag taatagaaaa agaaaattgc
     7981 gggaaaggac tgtgttatga cttccctgac taatgccgtg ttcaaacgat acctggcagt
     8041 gactcctagc gctcaccaag ctcttaaaac gggaatttat ggtgcactct cagtacaatc
     8101 tgctctgatg ccgcatagtt aagccagccc cgacacccgc caacacgcgc tgacgcgccc
     8161 tgacgggctt gtctgctccc ggcatccgct tacagacaag ctgtgaccgt ctccgggagc
     8221 tgcatgtgtc agaggttttc accgtcatca ccgaaacgcg cga
//
