LOCUS       sequence-127491-       10445 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Binary destination vector for plant transformation. Kanamycin 
            resistance..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10445)
  AUTHORS   Siligato R, Wang X, Yadav SR, Lehesranta S, Ma G, Ursache R, Sevilem
            I, Zhang J, Gorte M, Prasad K, Wrzaczek M, Heidstra R, Murphy A, 
            Scheres B, Mahonen AP
  TITLE     MultiSite Gateway compatible cell type-specific gene inducible 
            system for plants.
  JOURNAL   Plant Physiol. 2015 Dec 7. pii: pp.01246.2015.
  PUBMED    26644504
REFERENCE   2  (bases 1 to 10445)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Plant 
            Physiol. 2015 Dec 7. pii: pp.01246.2015."
COMMENT     Sequence Label: pCAM-kan-R4R3
FEATURES             Location/Qualifiers
     source          1..10445
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(42..58)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     misc_feature    261..285
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     CDS             1585..2214
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
     CDS             2648..3715
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="GRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESWQA
                     AADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLSKR
                     DRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKPGR
                     VFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGEAL
                     ISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYRLA
                     RRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPILV
                     MRYRNLIEGEASAGS"
     rep_origin      3781..3975
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
     primer_bind     complement(4211..4233)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     misc_feature    4319..4459
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     4374..4393
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     rep_origin      complement(4645..5233)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(4725..4744)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(5320..6114)
                     /codon_start=1
                     /gene="aphA-3"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM
                     TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED
                     EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
                     PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
                     FCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     misc_feature    6539..6563
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     terminator      complement(6638..6889)
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     CDS             complement(6929..7723)
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERGRTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTQGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(7040..7059)
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     7650..7669
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     promoter        complement(7757..7936)
                     /label=NOS promoter
                     /note="nopaline synthase promoter"
     primer_bind     8183..8199
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     8206..8225
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        8206..8224
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     8249..8265
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     primer_bind     8250..8266
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    8275..8398
                     /gene="mutant version of attR"
                     /label=attR4
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     CDS             complement(8439..8744)
                     /codon_start=1
                     /gene="ccdB"
                     /product="CcdB, a bacterial toxin that poisons DNA gyrase"
                     /label=ccdB
                     /note="Plasmids containing the ccdB gene cannot be 
                     propagated in standard E. coli strains."
                     /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK
                     VPRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     primer_bind     complement(8541..8560)
                     /label=ccdB-fwd
                     /note="ccdB gene, forward primer"
     CDS             complement(9086..9745)
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     9660..9679
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     promoter        complement(join(9799..9805,9806..9823,9824..9829))
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an ""up"" mutation"
     protein_bind    complement(9855..9978)
                     /gene="mutant version of attR"
                     /label=attR3
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(10003..10019)
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(10003..10019)
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     promoter        complement(10056..10074)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(10095..10111)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(10095..10111)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(10108..10130)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    10119..10135
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(10143..10149,10150..10168,10169..10174))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    10189..10210
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
ORIGIN
        1 ggggatcctc tagagtcgac ctgcaggcat gcaagcttgg cactggccgt cgttttacaa
       61 cgtcgtgact gggaaaaccc tggcgttacc caacttaatc gccttgcagc acatccccct
      121 ttcgccagct ggcgtaatag cgaagaggcc cgcaccgatc gcccttccca acagttgcgc
      181 agcctgaatg gcgaatgcta gagcagcttg agcttggatc agattgtcgt ttcccgcctt
      241 cagtttaaac tatcagtgtt tgacaggata tattggcggg taaacctaag agaaaagagc
      301 gtttattaga ataacggata tttaaaaggg cgtgaaaagg tttatccgtt cgtccatttg
      361 tatgtgcatg ccaaccacag ggttcccctc gggatcaaag tactttgatc caacccctcc
      421 gctgctatag tgcagtcggc ttctgacgtt cagtgcagcc gtcttctgaa aacgacatgt
      481 cgcacaagtc ctaagttacg cgacaggctg ccgccctgcc cttttcctgg cgttttcttg
      541 tcgcgtgttt tagtcgcata aagtagaata cttgcgacta gaaccggaga cattacgcca
      601 tgaacaagag cgccgccgct ggcctgctgg gctatgcccg cgtcagcacc gacgaccagg
      661 acttgaccaa ccaacgggcc gaactgcacg cggccggctg caccaagctg ttttccgaga
      721 agatcaccgg caccaggcgc gaccgcccgg agctggccag gatgcttgac cacctacgcc
      781 ctggcgacgt tgtgacagtg accaggctag accgcctggc ccgcagcacc cgcgacctac
      841 tggacattgc cgagcgcatc caggaggccg gcgcgggcct gcgtagcctg gcagagccgt
      901 gggccgacac caccacgccg gccggccgca tggtgttgac cgtgttcgcc ggcattgccg
      961 agttcgagcg ttccctaatc atcgaccgca cccggagcgg gcgcgaggcc gccaaggccc
     1021 gaggcgtgaa gtttggcccc cgccctaccc tcaccccggc acagatcgcg cacgcccgcg
     1081 agctgatcga ccaggaaggc cgcaccgtga aagaggcggc tgcactgctt ggcgtgcatc
     1141 gctcgaccct gtaccgcgca cttgagcgca gcgaggaagt gacgcccacc gaggccaggc
     1201 ggcgcggtgc cttccgtgag gacgcattga ccgaggccga cgccctggcg gccgccgaga
     1261 atgaacgcca agaggaacaa gcatgaaacc gcaccaggac ggccaggacg aaccgttttt
     1321 cattaccgaa gagatcgagg cggagatgat cgcggccggg tacgtgttcg agccgcccgc
     1381 gcacgtctca accgtgcggc tgcatgaaat cctggccggt ttgtctgatg ccaagctggc
     1441 ggcctggccg gccagcttgg ccgctgaaga aaccgagcgc cgccgtctaa aaaggtgatg
     1501 tgtatttgag taaaacagct tgcgtcatgc ggtcgctgcg tatatgatgc gatgagtaaa
     1561 taaacaaata cgcaagggga acgcatgaag gttatcgctg tacttaacca gaaaggcggg
     1621 tcaggcaaga cgaccatcgc aacccatcta gcccgcgccc tgcaactcgc cggggccgat
     1681 gttctgttag tcgattccga tccccagggc agtgcccgcg attgggcggc cgtgcgggaa
     1741 gatcaaccgc taaccgttgt cggcatcgac cgcccgacga ttgaccgcga cgtgaaggcc
     1801 atcggccggc gcgacttcgt agtgatcgac ggagcgcccc aggcggcgga cttggctgtg
     1861 tccgcgatca aggcagccga cttcgtgctg attccggtgc agccaagccc ttacgacata
     1921 tgggccaccg ccgacctggt ggagctggtt aagcagcgca ttgaggtcac ggatggaagg
     1981 ctacaagcgg cctttgtcgt gtcgcgggcg atcaaaggca cgcgcatcgg cggtgaggtt
     2041 gccgaggcgc tggccgggta cgagctgccc attcttgagt cccgtatcac gcagcgcgtg
     2101 agctacccag gcactgccgc cgccggcaca accgttcttg aatcagaacc cgagggcgac
     2161 gctgcccgcg aggtccaggc gctggccgct gaaattaaat caaaactcat ttgagttaat
     2221 gaggtaaaga gaaaatgagc aaaagcacaa acacgctaag tgccggccgt ccgagcgcac
     2281 gcagcagcaa ggctgcaacg ttggccagcc tggcagacac gccagccatg aagcgggtca
     2341 actttcagtt gccggcggag gatcacacca agctgaagat gtacgcggta cgccaaggca
     2401 agaccattac cgagctgcta tctgaataca tcgcgcagct accagagtaa atgagcaaat
     2461 gaataaatga gtagatgaat tttagcggct aaaggaggcg gcatggaaaa tcaagaacaa
     2521 ccaggcaccg acgccgtgga atgccccatg tgtggaggaa cgggcggttg gccaggcgta
     2581 agcggctggg ttgtctgccg gccctgcaat ggcactggaa cccccaagcc cgaggaatcg
     2641 gcgtgacggt cgcaaaccat ccggcccggt acaaatcggc gcggcgctgg gtgatgacct
     2701 ggtggagaag ttgaaggccg cgcaggccgc ccagcggcaa cgcatcgagg cagaagcacg
     2761 ccccggtgaa tcgtggcaag cggccgctga tcgaatccgc aaagaatccc ggcaaccgcc
     2821 ggcagccggt gcgccgtcga ttaggaagcc gcccaagggc gacgagcaac cagatttttt
     2881 cgttccgatg ctctatgacg tgggcacccg cgatagtcgc agcatcatgg acgtggccgt
     2941 tttccgtctg tcgaagcgtg accgacgagc tggcgaggtg atccgctacg agcttccaga
     3001 cgggcacgta gaggtttccg cagggccggc cggcatggcc agtgtgtggg attacgacct
     3061 ggtactgatg gcggtttccc atctaaccga atccatgaac cgataccggg aagggaaggg
     3121 agacaagccc ggccgcgtgt tccgtccaca cgttgcggac gtactcaagt tctgccggcg
     3181 agccgatggc ggaaagcaga aagacgacct ggtagaaacc tgcattcggt taaacaccac
     3241 gcacgttgcc atgcagcgta cgaagaaggc caagaacggc cgcctggtga cggtatccga
     3301 gggtgaagcc ttgattagcc gctacaagat cgtaaagagc gaaaccgggc ggccggagta
     3361 catcgagatc gagctagctg attggatgta ccgcgagatc acagaaggca agaacccgga
     3421 cgtgctgacg gttcaccccg attacttttt gatcgatccc ggcatcggcc gttttctcta
     3481 ccgcctggca cgccgcgccg caggcaaggc agaagccaga tggttgttca agacgatcta
     3541 cgaacgcagt ggcagcgccg gagagttcaa gaagttctgt ttcaccgtgc gcaagctgat
     3601 cgggtcaaat gacctgccgg agtacgattt gaaggaggag gcggggcagg ctggcccgat
     3661 cctagtcatg cgctaccgca acctgatcga gggcgaagca tccgccggtt cctaatgtac
     3721 ggagcagatg ctagggcaaa ttgccctagc aggggaaaaa ggtcgaaaag gtctctttcc
     3781 tgtggatagc acgtacattg ggaacccaaa gccgtacatt gggaaccgga acccgtacat
     3841 tgggaaccca aagccgtaca ttgggaaccg gtcacacatg taagtgactg atataaaaga
     3901 gaaaaaaggc gatttttccg cctaaaactc tttaaaactt attaaaactc ttaaaacccg
     3961 cctggcctgt gcataactgt ctggccagcg cacagccgaa gagctgcaaa aagcgcctac
     4021 ccttcggtcg ctgcgctccc tacgccccgc cgcttcgcgt cggcctatcg cggccgctgg
     4081 ccgctcaaaa atggctggcc tacggccagg caatctacca gggcgcggac aagccgcgcc
     4141 gtcgccactc gaccgccggc gcccacatca aggcaccctg cctcgcgcgt ttcggtgatg
     4201 acggtgaaaa cctctgacac atgcagctcc cggagacggt cacagcttgt ctgtaagcgg
     4261 atgccgggag cagacaagcc cgtcagggcg cgtcagcggg tgttggcggg tgtcggggcg
     4321 cagccatgac ccagtcacgt agcgatagcg gagtgtatac tggcttaact atgcggcatc
     4381 agagcagatt gtactgagag tgcaccatat gcggtgtgaa ataccgcaca gatgcgtaag
     4441 gagaaaatac cgcatcaggc gctcttccgc ttcctcgctc actgactcgc tgcgctcggt
     4501 cgttcggctg cggcgagcgg tatcagctca ctcaaaggcg gtaatacggt tatccacaga
     4561 atcaggggat aacgcaggaa agaacatgtg agcaaaaggc cagcaaaagg ccaggaaccg
     4621 taaaaaggcc gcgttgctgg cgtttttcca taggctccgc ccccctgacg agcatcacaa
     4681 aaatcgacgc tcaagtcaga ggtggcgaaa cccgacagga ctataaagat accaggcgtt
     4741 tccccctgga agctccctcg tgcgctctcc tgttccgacc ctgccgctta ccggatacct
     4801 gtccgccttt ctcccttcgg gaagcgtggc gctttctcat agctcacgct gtaggtatct
     4861 cagttcggtg taggtcgttc gctccaagct gggctgtgtg cacgaacccc ccgttcagcc
     4921 cgaccgctgc gccttatccg gtaactatcg tcttgagtcc aacccggtaa gacacgactt
     4981 atcgccactg gcagcagcca ctggtaacag gattagcaga gcgaggtatg taggcggtgc
     5041 tacagagttc ttgaagtggt ggcctaacta cggctacact agaaggacag tatttggtat
     5101 ctgcgctctg ctgaagccag ttaccttcgg aaaaagagtt ggtagctctt gatccggcaa
     5161 acaaaccacc gctggtagcg gtggtttttt tgtttgcaag cagcagatta cgcgcagaaa
     5221 aaaaggatct caagaagatc ctttgatctt ttctacgggg tctgacgctc agtggaacga
     5281 aaactcacgt taagggattt tggtcatgca ttctaggtac taaaacaatt catccagtaa
     5341 aatataatat tttattttct cccaatcagg cttgatcccc agtaagtcaa aaaatagctc
     5401 gacatactgt tcttccccga tatcctccct gatcgaccgg acgcagaagg caatgtcata
     5461 ccacttgtcc gccctgccgc ttctcccaag atcaataaag ccacttactt tgccatcttt
     5521 cacaaagatg ttgctgtctc ccaggtcgcc gtgggaaaag acaagttcct cttcgggctt
     5581 ttccgtcttt aaaaaatcat acagctcgcg cggatcttta aatggagtgt cttcttccca
     5641 gttttcgcaa tccacatcgg ccagatcgtt attcagtaag taatccaatt cggctaagcg
     5701 gctgtctaag ctattcgtat agggacaatc cgatatgtcg atggagtgaa agagcctgat
     5761 gcactccgca tacagctcga taatcttttc agggctttgt tcatcttcat actcttccga
     5821 gcaaaggacg ccatcggcct cactcatgag cagattgctc cagccatcat gccgttcaaa
     5881 gtgcaggacc tttggaacag gcagctttcc ttccagccat agcatcatgt ccttttcccg
     5941 ttccacatca taggtggtcc ctttataccg gctgtccgtc atttttaaat ataggttttc
     6001 attttctccc accagcttat ataccttagc aggagacatt ccttccgtat cttttacgca
     6061 gcggtatttt tcgatcagtt ttttcaattc cggtgatatt ctcattttag ccatttatta
     6121 tttccttcct cttttctaca gtatttaaag ataccccaag aagctaatta taacaagacg
     6181 aactccaatt cactgttcct tgcattctaa aaccttaaat accagaaaac agctttttca
     6241 aagttgtttt caaagttggc gtataacata gtatcgacgg agccgatttt gaaaccgcgg
     6301 tgatcacagg cagcaacgct ctgtcatcgt tacaatcaac atgctaccct ccgcgagatc
     6361 atccgtgttt caaacccggc agcttagttg ccgttcttcc gaatagcatc ggtaacatga
     6421 gcaaagtctg ccgccttaca acggctctcc cgctgacgcc gtcccggact gatgggctgc
     6481 ctgtatcgag tggtgatttt gtgccgagct gccggtcggg gagctgttgg ctggctggtg
     6541 gcaggatata ttgtggtgta aacaaattga cgcttagaca acttaataac acattgcgga
     6601 cgtttttaat gtactgaatt aacgccgaat tccggtcgat ctagtaacat agatgacacc
     6661 gcgcgcgata atttatccta gtttgcgcgc tatattttgt tttctatcgc gtattaaatg
     6721 tataattgcg ggactctaat caaaaaaccc atctcataaa taacgtcatg cattacatgt
     6781 taattattac atgcttaacg taattcaaca gaaattatat gataatcatc gcaagaccgg
     6841 caacaggatt caatcttaag aaactttatt gccaaatgtt tgaacgatct gcttgactct
     6901 agctagagtc cgaaccccag agtcccgctc agaagaactc gtcaagaagg cgatagaagg
     6961 cgatgcgctg cgaatcggga gcggcgatac cgtaaagcac gaggaagcgg tcagcccatt
     7021 cgccgccaag ctcttcagca atatcacggg tagccaacgc tatgtcctga tagcggtccg
     7081 ccacacccag ccggccacag tcgatgaatc cagaaaagcg gccattttcc accatgatat
     7141 tcggcaagca ggcatcgccc tgggtcacga cgagatcctc gccgtcgggc atccgcgcct
     7201 tgagcctggc gaacagttcg gctggcgcga gcccctgatg ctcttcgtcc agatcatcct
     7261 gatcgacaag accggcttcc atccgagtac gtcctcgctc gatgcgatgt ttcgcttggt
     7321 ggtcgaatgg gcaggtagcc ggatcaagcg tatgcagccg ccgcattgca tcagccatga
     7381 tggatacttt ctcggcagga gcaaggtgag atgacaggag atcctgcccc ggcacttcgc
     7441 ccaatagcag ccagtccctt cccgcttcag tgacaacgtc gagcacagct gcgcaaggaa
     7501 cgcccgtcgt ggccagccac gatagccgcg ctgcctcgtc ttggagttca ttcagggcac
     7561 cggacaggtc ggtcttgaca aaaagaaccg ggcgcccctg cgctgacagc cggaacacgg
     7621 cggcatcaga gcagccgatt gtctgttgtg cccagtcata gccgaatagc ctctccaccc
     7681 aagcggccgg agaacctgcg tgcaatccat cttgttcaat catgcctcga tcgagttgag
     7741 agtgaatatg agactctaat tggataccga ggggaattta tggaacgtca gtggagcatt
     7801 tttgacaaga aatatttgct agctgatagt gaccttaggc gacttttgaa cgcgcaataa
     7861 tggtttctga cgtatgtgct tagctcatta aactccagaa acccgcggct gagtggctcc
     7921 ttcaacgttg cggttctgtc agttccaaac gtaaaacggc ttgtcccgcg tcatcggcgg
     7981 gggtcataac gtgactccct taattctcat gtatcgataa cattaacgtt tacaatttcg
     8041 cgccattcgc cattcaggct gcgcaactgt tgggaagggc gatcggtgcg ggcctcttcg
     8101 ctattacgcc agctggcgaa agggggatgt gctgcaaggc gattaagttg ggtaacgcca
     8161 gggttttccc agtcacgacg ttgtaaaacg acggccagtg aattgtaata cgactcacta
     8221 tagggcgaat tgggtaccgg gccccccctc gaggtcgacg gtatcgataa gcttcaactt
     8281 tgtatagaaa agttgaacga gaaacgtaaa atgatataaa tatcaatata ttaaattaga
     8341 ttttgcataa aaaacagact acataatact gtaaaacaca acatatccag tcactatggt
     8401 cgacctgcag actggctgtg tataagggag cctgacattt atattcccca gaacatcagg
     8461 ttaatggcgt ttttgatgtc attttcgcgg tggctgagat cagccacttc ttccccgata
     8521 acggagaccg gcacactggc catatcggtg gtcatcatgc gccagctttc atccccgata
     8581 tgcaccaccg ggtaaagttc acgggggact ttatctgaca gcagacgtgc actggccagg
     8641 gggatcacca tccgtcgccc gggcgtgtca ataatatcac tctgtacatc cacaaacaga
     8701 cgataacggc tctctctttt ataggtgtaa accttaaact gcatttcacc agcccctgtt
     8761 ctcgtcggca aaagagccgt tcatttcaat aaaccgggcg acctcagcca tcccttcctg
     8821 attttccgct ttccagcgtt cggcacgcag acgacgggct tcattctgca tggttgtgct
     8881 taccgaaccg gagatattga catcatatat gccttgagca actgatagct gtcgctgtca
     8941 actgtcactg taatacgctg cttcatagca tacctctttt tgacatactt cgggtataca
     9001 tatcagtata tattcttata ccgcaaaaat cagcgcgcaa atacgcatac tgttatctgg
     9061 cttttagtaa gccggatcct ctagattacg ccccgccctg ccactcatcg cagtactgtt
     9121 gtaattcatt aagcattctg ccgacatgga agccatcaca aacggcatga tgaacctgaa
     9181 tcgccagcgg catcagcacc ttgtcgcctt gcgtataata tttgcccatg gtgaaaacgg
     9241 gggcgaagaa gttgtccata ttggccacgt ttaaatcaaa actggtgaaa ctcacccagg
     9301 gattggctga gacgaaaaac atattctcaa taaacccttt agggaaatag gccaggtttt
     9361 caccgtaaca cgccacatct tgcgaatata tgtgtagaaa ctgccggaaa tcgtcgtggt
     9421 attcactcca gagcgatgaa aacgtttcag tttgctcatg gaaaacggtg taacaagggt
     9481 gaacactatc ccatatcacc agctcaccgt ctttcattgc catacggaat tccggatgag
     9541 cattcatcag gcgggcaaga atgtgaataa aggccggata aaacttgtgc ttatttttct
     9601 ttacggtctt taaaaaggcc gtaatatcca gctgaacggt ctggttatag gtacattgag
     9661 caactgactg aaatgcctca aaatgttctt tacgatgcca ttgggatata tcaacggtgg
     9721 tatatccagt gatttttttc tccattttag cttccttagc tcctgaaaat ctcgacggat
     9781 cctaactcaa aatccacaca ttatacgagc cggaagcata aagtgtaaag cctgggggtg
     9841 cctaatgcgg ccgccatagt gactggatat gttgtgtttt acagtattat gtagtctgtt
     9901 ttttatgcaa aatctaattt aatatattga tatttatatc attttacgtt tctcgttcaa
     9961 ctttattata catagttgga tatcgaattc ctgcagcccg gggatccact agttctagag
    10021 cggccgccac cgcggtggag ctccagcttt tgttcccttt agtgagggtt aattccgagc
    10081 ttggcgtaat catggtcata gctgtttcct gtgtgaaatt gttatccgct cacaattcca
    10141 cacaacatac gagccggaag hcataaagtg taaagcctgg ggtgcctaat gagtgagcta
    10201 actcacatta attgcgttgc gctcactgcc cgctttccag tcgggaaacc tgtcgtgcca
    10261 gctgcattaa tgaatcggcc aacgcgcggg gagaggcggt ttgcgtattg ggcgctcttc
    10321 cgcttcctcg ctcactgact cgctgcgctc ggtcgttcgg ctgcggcgag cggtatcagc
    10381 tcactcaaag gcggtaatac ggttatccac agaatcaggg gataacgcag gaaagaacat
    10441 gaagg
//
