LOCUS       sequence-127492-         505 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  CIS-V3m with N-FLAG and C-HIS.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 505)
  AUTHORS   Slomovic S, Collins JJ
  TITLE     DNA sense-and-respond protein modules for mammalian cells.
  JOURNAL   Nat Methods. 2015 Sep 21. doi: 10.1038/nmeth.3585.
  PUBMED    26389572
REFERENCE   2  (bases 1 to 505)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Methods. 2015 Sep 21. doi: 10.1038/nmeth.3585."
COMMENT     Sequence Label: pBW121-SS-281
FEATURES             Location/Qualifiers
     source          1..505
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(52..69)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             complement(88..237)
                     /codon_start=1
                     /product="tetrameric repeat of the minimal activation 
                     domain of herpes simplex virus VP16 (Beerli et al., 1998)"
                     /label=VP64
                     /translation="DALDDFDLDMLGSDALDDFDLDMLGSDALDDFDLDMLGSDALDDF
                     DLDML"
     CDS             complement(350..361)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
ORIGIN
        1 cagccaccac cttctgatag gcagcctgca cctgaggagt gcggccgctt aatggtgatg
       61 gtgatggtgg ccagatccac aattaattaa catatcgaga tcgaaatcgt ccagagcatc
      121 ggagccgagc atgtccagat caaagtcatc caatgcgtcg cttcccaaca tatccaggtc
      181 aaagtcatcg agggcatcag aacccagcat gtcgagatcg aaatcgtcca gcgcgtcggc
      241 gcgccctccg ctgccccaca gcacgctgtc gcaagcatgg acgacaacct ggttggcaag
      301 caaaaactga tgatcagaat catcagataa agtaatccca taataatcgt cttccttcaa
      361 ttcttgtaac tcgaaataaa atccgcggca ctcacgtgca aaagcagcgg cgggagcagg
      421 cctgaatttt ttagagccgg cacacttcga aagaacgtta agcaaaactg atccgcctcc
      481 tgatccgcca cctgatccgc cccca
//
