LOCUS       sequence-127496-         989 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses rat Cx43 (Gja1 CDS) truncated at amino acid 258 and tagged
            with msfGFP on the C-terminus. CMV promoter..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 989)
  AUTHORS   Stout RF Jr, Snapp EL, Spray DC
  TITLE     Connexin Type and Fluorescent Protein-fusion Tag Determine 
            Structural Stability of Gap Junction Plaques.
  JOURNAL   J Biol Chem. 2015 Aug 11. pii: jbc.M115.659979.
  PUBMED    26265468
REFERENCE   2  (bases 1 to 989)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Biol 
            Chem. 2015 Aug 11. pii: jbc.M115.659979."
COMMENT     Sequence Label: Cx43K258stop-msfGFP
FEATURES             Location/Qualifiers
     source          1..989
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      899..908
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     primer_bind     complement(950..971)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
ORIGIN
        1 agcagagctg gtttagtgaa ccgtcagatc cgctagcgct accggactca gatctcgagc
       61 tcaagcttcg aattctgcag tcgacggtac cgcgggcccg ggcaccatgg gtgactggag
      121 tgccttgggg aagcttctgg acaaggtcca agcctactcc accgctggag ggaaggtgtg
      181 gctgtcagtg ctcttcatat tcagaatcct gctcctgggg acagctgttg agtcagcttg
      241 gggtgatgaa cagtctgcct ttcgctgtaa cactcaacaa cctggctgcg aaaacgtctg
      301 ctatgacaag tccttcccca tctctcacgt gcgcttctgg gtccttcaga tcatattcgt
      361 gtctgtgccc accctcctgt acttggccca tgtgttctat gtgatgagga aggaagagaa
      421 gctaaacaag aaagaagagg agctcaaagt ggcccagact gacggggtca acgtggagat
      481 gcacctgaag cagattgaaa tcaagaagtt caagtacggg attgaagagc acggcaaggt
      541 gaaaatgagg ggcggcttgc tgagaaccta catcatcagc atcctcttca agtctgtctt
      601 cgaggtggcc ttcctgctca tccagtggta catctatggg ttcagcttga gcgcggtcta
      661 cacctgcaag agagatccct gcccccacca ggtagactgc ttcctctcac gtcccacgga
      721 gaaaaccatc ttcatcatct tcatgctggt ggtgtccttg gtgtctctcg ctttgaacat
      781 cattgagctc ttctacgtct tcttcaaagg cgttaaggat cgcgtgaagg gaagaagcga
      841 tccttaccac gccaccactg gcccactgag cccatcatcc gcccgggatc caccggtcgc
      901 caccatggtg agcaagggcg aggagctgtt caccggggtg gtgcccatcc tggtcgagct
      961 ggacggcgac gtaaacggcc acaagttca
//
