LOCUS       sequence-127497-        1030 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses rat Cx43 (Gja1 CDS) truncated at amino acid 258 and tagged
            with msfGFP on the C-terminus. CMV promoter..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1030)
  AUTHORS   Stout RF Jr, Snapp EL, Spray DC
  TITLE     Connexin Type and Fluorescent Protein-fusion Tag Determine 
            Structural Stability of Gap Junction Plaques.
  JOURNAL   J Biol Chem. 2015 Aug 11. pii: jbc.M115.659979.
  PUBMED    26265468
REFERENCE   2  (bases 1 to 1030)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Biol 
            Chem. 2015 Aug 11. pii: jbc.M115.659979."
COMMENT     Sequence Label: Cx43K258stop-msfGFP
FEATURES             Location/Qualifiers
     source          1..1030
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(join(8..721,722..724,725..727))
                     /codon_start=1
                     /product="GFP variant that folds robustly even when fused 
                     to poorly folded proteins (Pedelacq et al., 2006)"
                     /label=superfolder GFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVRGEGEGDATNGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKRHDFFKSAMPEGYVQERTISFKDD
                     GTYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNFNSHNVYITADKQKNGIK
                     ANFKIRHNVEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSKLSKDPNEKRDHMVLL
                     EFVTAAGITHGMDELYK"
     primer_bind     complement(53..74)
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     661..682
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     regulatory      724..733
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 ggccgcttta cttgtacagc tcgtccatgc cgtgagtgat cccggcggcg gtcacgaact
       61 ccagcaggac catgtgatcg cgcttctcgt tggggtcttt gctcagcttg gactgggtgc
      121 tcaggtagtg gttgtcgggc agcagcacgg ggccgtcgcc gatgggggtg ttctgctggt
      181 agtggtcggc gagctgcacg ctgccgtcct ccacgttgtg gcggatcttg aagttggcct
      241 tgatgccgtt cttctgcttg tcggcggtga tatagacgtt gtggctgttg aagttgtact
      301 ccagcttgtg ccccaggatg ttgccgtcct ccttgaagtc gatgcccttc agctcgatgc
      361 ggttcaccag ggtgtcgccc tcgaacttca cctcggcgcg ggtcttgtag gtgccgtcgt
      421 ccttgaagct gatggtgcgc tcctggacgt agccttcggg catggcggac ttgaagaagt
      481 cgtggcgctt catgtggtcg gggtagcggc tgaagcactg cacgccgtag gtcagggtgg
      541 tcacgagggt gggccagggc acgggcagct tgccggtggt gcagatgaac ttcagggtca
      601 gcttgccgtt ggtggcatcg ccctcgccct cgccgcgcac gctgaacttg tggccgttta
      661 cgtcgccgtc cagctcgacc aggatgggca ccaccccggt gaacagctcc tcgcccttgc
      721 tcaccatggt ggcgaccggt ggatcccggg cggatgatgg gctcagtggg ccagtggtgg
      781 cgtggtaagg atcgcttctt cccttcacgc gatccttaac gcctttgaag aagacgtaga
      841 agagctcaat gatgttcaaa gcgagagaca ccaaggacac caccagcatg aagatgatga
      901 agatggtttt ctccgtggga cgtgagagga agcagtctac ctggtggggg cagggatctc
      961 tcttgcaggt gtagaccgcg ctcaagctga acccatagat gtaccactgg atgagcagga
     1021 aggccacctc
//
