LOCUS       sequence-127499-         876 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  8x Target 1 12gap minus SV40 Ori.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 876)
  AUTHORS   Slomovic S, Collins JJ
  TITLE     DNA sense-and-respond protein modules for mammalian cells.
  JOURNAL   Nat Methods. 2015 Sep 21. doi: 10.1038/nmeth.3585.
  PUBMED    26389572
REFERENCE   2  (bases 1 to 876)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Methods. 2015 Sep 21. doi: 10.1038/nmeth.3585."
COMMENT     Sequence Label: pBW121-SS-311
FEATURES             Location/Qualifiers
     source          1..876
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    569..590
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(605..610,611..628,629..635)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    643..659
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     648..670
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     667..683
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     667..683
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
ORIGIN
        1 cccgcgcgtt ggccgattca ttaatgcagc ggatctctcg agttaatttc gcttaatttc
       61 gcggatccgg accacttccc ctccagctga ctgactgaca cctcctgcag cttcttcaag
      121 atccggacca cttcccctcc agctgactga ctgacacctc ctgcagcttc ttcaagatcc
      181 ggaccacttc ccctccagct gactgactga cacctcctgc agcttcttca agatccggac
      241 cacttcccct ccagctgact gactgacacc tcctgcagct tcttcaagat ccggaccact
      301 tcccctccag ctgactgact gacacctcct gcagcttctt caagatccgg accacttccc
      361 ctccagctga ctgactgaca cctcctgcag cttcttcaag atccggacca cttcccctcc
      421 agctgactga ctgacacctc ctgcagcttc ttcaagatcc ggaccacttc ccctccagct
      481 gactgactga cacctcctgc agcttcttca agatccgctg gcacgacagg tttcccgact
      541 ggaaagcggg cagtgagcgc aacgcaatta atgtgagtta gctcactcat taggcacccc
      601 aggctttaca ctttatgctt ccggctcgta tgttgtgtgg aattgtgagc ggataacaat
      661 ttcacacagg aaacagctat gaccatgatt acgccaagct tgggctgcag gtcgagggat
      721 ctccataaga gaagagggac agctatgact gggagtagtc aggagaggag gaaaaatctg
      781 gctagtaaaa catgtaagga aaattttagg gatgttaaag aaaaaaataa cacaaaacaa
      841 aatataaaaa aaatctaacc tcaagtcaag gctttt
//
