LOCUS       sequence-127504-         879 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Tublin-targetted Kohinoor for mammalian cell expression.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 879)
  AUTHORS   Tiwari DK, Arai Y, Yamanaka M, Matsuda T, Agetsuma M, Nakano M, 
            Fujita K, Nagai T
  TITLE     A fast- and positively photoswitchable fluorescent protein for 
            ultralow-laser-power RESOLFT nanoscopy.
  JOURNAL   Nat Methods. 2015 Jun;12(6):515-8. doi: 10.1038/nmeth.3362. Epub 
            2015 Apr 20.
  PUBMED    25894946
REFERENCE   2  (bases 1 to 879)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/nmeth.3362"; journalName: "Nat Methods"; date: "2015-06"; 
            volume: "12"; issue: "6"; pages: "515-8"
COMMENT     Sequence Label: Kohinoor-Tublin/pcDNA3
FEATURES             Location/Qualifiers
     source          1..879
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     35..54
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        35..53
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
ORIGIN
        1 ctagagaacc cactgcttac tggcttatcg aaattaatac gactcactat agggagaccc
       61 aagcttgcca ccatgaggga aatcgtgcac atccaggccg gacagtgtgg caaccagatc
      121 ggtgctaagt tctgggaggt gataagcgat gaacatggca tcgaccccac cggtacctac
      181 cacggtgaca gcgacctgca gctggaccga atctctgtgt actataatga agccacaggt
      241 ggcaagtatg tccctcgagc tatcttggtg gatctagaac ctgggactat ggactccgtt
      301 cgctcaggtc cttttggcca gatcttcaga ccagacaact tcgttttcgg tcagtctggg
      361 gcaggcaaca actgggctaa aggccactac acagagggag ctgagttggt tgactctgtc
      421 ttggatgtgg tgcggaagga ggcggagagc tgtgattgcc tgcaaggctt tcagctgacc
      481 cactcactgg gtggaggcac tggctctggc atgggcaccc tgctcatcag caagatccgg
      541 gaagaatatc ctgaccgtat catgaatacc ttcagtgtgg tgccctcgcc caaagtgtct
      601 gataccgtgg tcgagcccta caatgccacc ctgtctgtcc atcagttggt tgagaacacg
      661 gatgagacct actgcatcga caacgaggcc ctctacgaca tctgcttccg taccctcaag
      721 ctcaccacgc caacctacgg agacctgaac catctcgtct cggccaccat gagcggcgtc
      781 accacctgcc tccgtttccc gggccagctt aatgctgacc ttcgaaagct ggctgtcaac
      841 atggtgccat tcccacgtct ccacttcttc atgcctggc
//
