LOCUS       sequence-127507-         888 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Tublin-targetted Kohinoor for mammalian cell expression.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 888)
  AUTHORS   Tiwari DK, Arai Y, Yamanaka M, Matsuda T, Agetsuma M, Nakano M, 
            Fujita K, Nagai T
  TITLE     A fast- and positively photoswitchable fluorescent protein for 
            ultralow-laser-power RESOLFT nanoscopy.
  JOURNAL   Nat Methods. 2015 Jun;12(6):515-8. doi: 10.1038/nmeth.3362. Epub 
            2015 Apr 20.
  PUBMED    25894946
REFERENCE   2  (bases 1 to 888)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/nmeth.3362"; journalName: "Nat Methods"; date: "2015-06"; 
            volume: "12"; issue: "6"; pages: "515-8"
COMMENT     Sequence Label: Kohinoor-Tublin/pcDNA3
FEATURES             Location/Qualifiers
     source          1..888
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(41..715)
                     /codon_start=1
                     /product="fast-switching photoswitchable fluorescent 
                     protein useful for fluorescence nanoscopy (Tiwari et al., 
                     2015)"
                     /label=Kohinoor
                     /translation="MSVIKPDMKIKLRMEGAVNGHPFAIEGVGLGKPFEGKQSMDLKVK
                     EGGPLPFAYDILTMAFCYGNRVFAKYPENIVDYFKQSFPEGYSWERSMIYEDGGICIAT
                     NDITLDGDCYIYEIRFDGVNFPANGPVMQKRTVKWEPSTEKLYVRDGVLKSDGNYALSL
                     EGGGHYRCDSKTTYKAKKVVQLPDYHDVVHHIEIKSHDRDYSNVNLHEHAEAHSGLPRQ
                     AK"
ORIGIN
        1 tatagaatag ggccctctag atgcatgctc gagcggccgc ttacttggcc tgcctcggca
       61 gcccagaatg cgcttcggca tgctcatgca gattaacatt actgtaatct ctgtcgtggc
      121 ttttaatctc aatgtggtgg accacatcgt gatagtctgg caactggaca accttcttag
      181 ctttataagt agttttggag tcacatcggt agtggccacc tccttcaagc gacagagcgt
      241 agttgccatc gctcttcagc actccatcac gcacatacaa tttctcagtg gatggctccc
      301 atttcacagt cctcttctgc ataactggac cattggcagg aaagttcaca ccatcaaatc
      361 gaatttcata gatataacag tcaccatcca gggttatgtc gtttgtcgcg atacaaatgc
      421 ccccgtcttc gtaaatcatg cttcgttccc aagagtagcc ctcaggaaac gactgcttga
      481 aataatctac tatattttct gggtatttgg cgaataccct gttgccgtaa cagaaggcca
      541 ttgtcaagat gtcataggcg aaaggcagag gtccgccttc tttgacttta aggtccatac
      601 tctgttttcc ctcgaaaggc ttcccaaggc caactccttc aatcgcgaac gggtgtccat
      661 ttacagcgcc ttccatacgc agcttgatct tcatgtctgg tttaatcaca ctcattggat
      721 cccgggcccg cggtaccgtc gactgggcct cctcttctgc ctcctctccg aaatcctctt
      781 cctcttccgc ggtggcatcc tggtactgct ggtactcaga gacgaggtcg ttcatgttgc
      841 tctcagcttc ggtgaactcc atctcgtcca tgccctcacc cgtatacc
//
