LOCUS       sequence-127518-         403 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Provides the 4xLexA Operator fused to CaMV 35s minimal promoter as a
            level 0 GoldenBraid part.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 403)
  AUTHORS   Sarrion-Perdigones A, Vazquez-Vilar M, Palaci J, Castelijns B, 
            Forment J, Ziarsolo P, Blanca J, Granell A, Orzaez D
  TITLE     GoldenBraid 2.0: a comprehensive DNA assembly framework for plant 
            synthetic biology.
  JOURNAL   Plant Physiol. 2013 Jul;162(3):1618-31.
  PUBMED    23669743
REFERENCE   2  (bases 1 to 403)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Plant 
            Physiol."; date: "2013-07"; volume: "162(3)"; pages: "1618-31"
COMMENT     Sequence Label: pOpLexA::mini35s (GB0733)
FEATURES             Location/Qualifiers
     source          1..403
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      5..14
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     regulatory      9..18
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     promoter        complement(129..175)
                     /label=minimal CaMV 35S promoter
                     /note="minimal 35S promoter from cauliflower mosaic virus"
ORIGIN
        1 tccggccgcc atggcggccg cgggaattcg attgggcgat gagtggtctc gcatttcgac
       61 tagaatagta aattgtaatg ttgtttgttg tttgttttgt tgtggtaatt gttgtaaaaa
      121 taccggagtc ctctccaaat gaaatgaact tccttatata gaggaagggt cttgcgaagg
      181 ggagcatatt acatggcatt accaccatat acatatccat atacatatcc atatctaatc
      241 ttacctcgac tgctgtatat aaaaccagtg gttatatgta cagtactgct gtatataaaa
      301 ccagtggtta tatgtacagt acgtcgactg ctgtatataa aaccagtggt tatatgtaca
      361 gtactgctgt atataaaacc agtggttata tgtacagtac gtc
//
