LOCUS       sequence-127519-         955 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression of His-tagged Grx1-roGFP2.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 955)
  AUTHORS   Gutscher M, Pauleau AL, Marty L, Brach T, Wabnitz GH, Samstag Y, 
            Meyer AJ, Dick TP
  TITLE     Real-time imaging of the intracellular glutathione redox potential.
  JOURNAL   Nat Methods. 2008 Jun;5(6):553-9. doi: 10.1038/nmeth.1212. Epub 2008
            May 11.
  PUBMED    18469822
REFERENCE   2  (bases 1 to 955)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/nmeth.1212"; journalName: "Nat Methods"; date: "2008-06"; 
            volume: "5"; issue: "6"; pages: "553-9"
COMMENT     Sequence Label: pQE-60 Grx1-roGFP2-His
FEATURES             Location/Qualifiers
     source          1..955
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    13..29
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     RBS             48..59
                     /note="strong bacterial ribosome binding site (Elowitz and 
                     Leibler, 2000)"
     CDS             join(486..488,489..947)
                     /codon_start=1
                     /product="improved N-terminal fragment of mVenus for use in
                     bimolecular fluorescence complementation (BiFC) (Kodama and
                     Hu, 2010)"
                     /label=VN155(I152L)
                     /translation="VSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FISTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNCHNVYIMA"
     primer_bind     complement(528..549)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(789..808)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
ORIGIN
        1 aatttatttg ctttgtgagc ggataacaat ttcacacaga attcattaaa gaggagaaat
       61 taaccatggc tcaagagttt gtgaactgca aaatccagcc tgggaaggtg gttgtgttca
      121 tcaagcccac ctgcccgtac tgcaggaggg cccaagagat cctcagtcaa ttgcccatca
      181 aacaagggct tctggaattt gtcgatatca cagccaccaa ccacactaac gagattcaag
      241 attatttgca acagctcacg ggagcaagaa cggtgcctcg agtctttatt ggtaaagatt
      301 gtataggcgg atgcagtgat ctagtctctt tgcaacagag tggggaactg ctgacgcggc
      361 taaagcagat tggagctctg cagactagtg gtggttcagg tggtggtggt tcaggtggtg
      421 gtggttcagg tggaggagga tcaggaggag gaggatcagg aggaggagga tcaggaggag
      481 aattcgtgag caagggcgag gagctgttca ccggggtggt gcccatcctg gtcgagctgg
      541 acggcgacgt aaacggccac aagttcagcg tgtccggcga gggcgagggc gatgccacct
      601 acggcaagct gaccctgaag ttcatctcca ccaccggcaa gctgcccgtg ccctggccca
      661 ccctcgtgac caccctgacc tacggcgtgc agtgcttcag ccgctacccc gaccacatga
      721 agcagcacga cttcttcaag tccgccatgc ccgaaggcta cgtccaggag cgcaccatct
      781 tcttcaagga cgacggcaac tacaagaccc gcgccgaggt gaagttcgag ggcgacaccc
      841 tggtgaaccg catcgagctg aagggcatcg acttcaagga ggacggcaac atcctggggc
      901 acaagctgga gtacaactac aactgccaca acgtctatat catggccgac aagca
//
