LOCUS       sequence-127522-         685 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression of His-tagged Grx1-roGFP2.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 685)
  AUTHORS   Gutscher M, Pauleau AL, Marty L, Brach T, Wabnitz GH, Samstag Y, 
            Meyer AJ, Dick TP
  TITLE     Real-time imaging of the intracellular glutathione redox potential.
  JOURNAL   Nat Methods. 2008 Jun;5(6):553-9. doi: 10.1038/nmeth.1212. Epub 2008
            May 11.
  PUBMED    18469822
REFERENCE   2  (bases 1 to 685)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/nmeth.1212"; journalName: "Nat Methods"; date: "2008-06"; 
            volume: "5"; issue: "6"; pages: "553-9"
COMMENT     Sequence Label: pQE-60 Grx1-roGFP2-His
FEATURES             Location/Qualifiers
     source          1..685
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     289..310
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     CDS             359..376
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     terminator      399..493
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     primer_bind     complement(603..622)
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
ORIGIN
        1 ccgcatcgag ctgaagggca tcgacttcaa ggaggacggc aacatcctgg ggcacaagct
       61 ggagtacaac tacaactgcc acaacgtcta tatcatggcc gacaagcaga agaacggcat
      121 caaggtgaac ttcaagatcc gccacaacat cgaggacggc agcgtgcagc tcgccgacca
      181 ctaccagcag aacaccccca tcggcgacgg ccccgtgctg ctgcccgaca accactacct
      241 gagcacctgc tccgccctga gcaaagaccc caacgagaag cgcgatcaca tggtcctgct
      301 ggagttcgtg accgccgccg ggatcactct cggcatggac gagctgtaca agagatctca
      361 tcaccatcac catcactaag cttaattagc tgagcttgga ctcctgttga tagatccagt
      421 aatgacctca gaactccatc tggatttgtt cagaacgctc ggttgccgcc gggcgttttt
      481 tattggtgag aatccaagct agcttggcga gattttcagg agctaaggaa gctaaaatgg
      541 agaaaaaaat cactggatat accaccgttg atatatccca atggcatcgt aaagaacatt
      601 ttgaggcatt tcagtcagtt gctcaatgta cctataacca gaccgttcag ctggatatta
      661 cggccttttt aaagaccgta aagaa
//
