LOCUS       sequence-127524-         994 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses rat Cx43 (Gja1 CDS) truncated at amino acid 258 and tagged
            with sfGFP (V206-version) on the N-terminus. CMV promoter..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 994)
  AUTHORS   Stout RF Jr, Snapp EL, Spray DC
  TITLE     Connexin Type and Fluorescent Protein-fusion Tag Determine 
            Structural Stability of Gap Junction Plaques.
  JOURNAL   J Biol Chem. 2015 Aug 11. pii: jbc.M115.659979.
  PUBMED    26265468
REFERENCE   2  (bases 1 to 994)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Biol 
            Chem. 2015 Aug 11. pii: jbc.M115.659979."
COMMENT     Sequence Label: sfGFP-Cx43K258stop
FEATURES             Location/Qualifiers
     source          1..994
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(899..920)
                     /label=EGFP-C
                     /note="EGFP, forward primer"
ORIGIN
        1 gatccggtgg atcccgggcc cgcggtaccg tcgactgcag aattcgaagc ttgagctctt
       61 atgatgggct cagtgggcca gtggtggcgt ggtaaggatc gcttcttccc ttcacgcgat
      121 ccttaacgcc tttgaagaag acgtagaaga gctcaatgat gttcaaagcg agagacacca
      181 aggacaccac cagcatgaag atgatgaaga tggttttctc cgtgggacgt gagaggaagc
      241 agtctacctg gtgggggcag ggatctctct tgcaggtgta gaccgcgctc aagctgaacc
      301 catagatgta ccactggatg agcaggaagg ccacctcgaa gacagacttg aagaggatgc
      361 tgatgatgta ggttctcagc aagccgcccc tcattttcac cttgccgtgc tcttcaatcc
      421 cgtacttgaa cttcttgatt tcaatctgct tcaggtgcat ctccacgttg accccgtcag
      481 tctgggccac tttgagctcc tcttctttct tgtttagctt ctcttccttc ctcatcacat
      541 agaacacatg ggccaagtac aggagggtgg gcacagacac gaatatgatc tgaaggaccc
      601 agaagcgcac gtgagagatg gggaaggact tgtcatagca gacgttttcg cagccaggtt
      661 gttgagtgtt acagcgaaag gcagactgtt catcacccca agctgactca acagctgtcc
      721 ccaggagcag gattctgaat atgaagagca ctgacagcca caccttccct ccagcggtgg
      781 agtaggcttg gaccttgtcc agaagcttcc ccaaggcact ccagtcaccc atctgagctc
      841 gagatctgag tccggacttg tacagctcgt ccatgccgtg agtgatcccg gcggcggtca
      901 cgaactccag caggaccatg tgatcgcgct tctcgttggg gtctttgctc agcacggact
      961 gggtgctcag gtagtggttg tcgggcagca gcac
//
