LOCUS       sequence-127527-         740 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Single construct Sensor pair 1, ZF9 Op x6 -- GFP, with mCherry 
            marker.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 740)
  AUTHORS   Slomovic S, Collins JJ
  TITLE     DNA sense-and-respond protein modules for mammalian cells.
  JOURNAL   Nat Methods. 2015 Sep 21. doi: 10.1038/nmeth.3585.
  PUBMED    26389572
REFERENCE   2  (bases 1 to 740)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Methods. 2015 Sep 21. doi: 10.1038/nmeth.3585."
COMMENT     Sequence Label: pVITRO1-SS-338
FEATURES             Location/Qualifiers
     source          1..740
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     218..239
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     regulatory      281..290
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     promoter        complement(317..348)
                     /label=minP
                     /note="minimal TATA-box promoter with low basal activity"
     misc_feature    474..565
                     /label=pause site
                     /note="RNA polymerase II transcriptional pause signal from 
                     the human alpha-2 globin gene"
     primer_bind     complement(506..525)
                     /label=RVprimer3
                     /note="pGL3 vector, forward primer"
     polyA_signal    579..627
                     /note="synthetic polyadenylation signal"
ORIGIN
        1 tggacgtagc cttcgggcat ggcggacttg aagaagtcgt gctgcttcat gtggtcgggg
       61 tagcggctga agcactgcac gccgtaggtc agggtggtca cgagggtggg ccagggcacg
      121 ggcagcttgc cggtggtgca gatgaacttc agggtcagct tgccgtaggt ggcatcgccc
      181 tcgccctcgc cggacacgct gaacttgtgg ccgtttacgt cgccgtccag ctcgaccagg
      241 atgggcacca ccccggtgaa cagctcctcg cccttgctca ccatggtggc tttaccaaca
      301 gtaccggatt gccaagctgg aagtcgagct tccattatat accctctagt gtctaagctt
      361 ctccatctac atttctccat ctacatttct ccatctacat ttctccatct acatttctcc
      421 atctacattt ctccatctac agctagcgag ctcaggtacc ggccagttag gccagagaaa
      481 tgttctggca cctgcacttg cactggggac agcctatttt gctagtttgt tttgtttcgt
      541 tttgttttga tggagagcgt atgttagtac tatcgattca cacaaaaaac caacacacag
      601 atgtaatgaa aataaagata ttttattagt ttaaacaaga acatgtgagc aaaaggccag
      661 caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt ttttccatag gctccgcccc
      721 cctgacgagc atcacaaaaa
//
