LOCUS       sequence-127532-        1059 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses rat Cx26 (Gjb2 CDS) with a 7 amino acid linker on the 
            C-terminus of the Connexin linking to non-monomerized superfolder 
            GFP. Expression driven by CMV promoter..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1059)
  AUTHORS   Stout RF Jr, Snapp EL, Spray DC
  TITLE     Connexin Type and Fluorescent Protein-fusion Tag Determine 
            Structural Stability of Gap Junction Plaques.
  JOURNAL   J Biol Chem. 2015 Aug 11. pii: jbc.M115.659979.
  PUBMED    26265468
REFERENCE   2  (bases 1 to 1059)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Biol 
            Chem. 2015 Aug 11. pii: jbc.M115.659979."
COMMENT     Sequence Label: Cx26-sfGFP
FEATURES             Location/Qualifiers
     source          1..1059
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      657..666
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     primer_bind     complement(708..729)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
ORIGIN
        1 tcaacaagca ctccaccagc attgggaaaa tctggctcac tgtcctcttc atcttccgca
       61 tcatgatcct cgtggtggcc gcgaaggagg tgtggggaga tgagcaagcc gattttgttt
      121 gcaacactct ccagcctggc tgtaagaatg tgtgctacga ccactacttc cccatctctc
      181 acatccggct ctgggctctg cagctgatca tggtgtccac gccggccctc ctggtagcta
      241 tgcacgtggc ctaccggaga cacgaaaaga aacggaagtt catgaaggga gagataaaga
      301 acgagtttaa ggacatcgaa gagatcaaaa cccagaaggt ccgtatcgaa gggtccctgt
      361 ggtggaccta caccaccagc atcttcttcc gggtcatctt cgaagctgtc ttcatgtatg
      421 tcttttacat catgtacaat ggcttcttca tgcagcgtct ggtgaagtgt aacgcctggc
      481 cttgtcccaa tacagtggac tgcttcattt ccaggcccac agaaaagact gtcttcacgg
      541 tgttcatgat ctctgtgtct ggaatttgca tcctgctaaa catcacagag ctgtgctatc
      601 tgttcattag gtattgctca gggaagtcca aaagaccagt cgcggatcca ccggtcgcca
      661 ccatggtgag caagggcgag gagctgttca ccggggtggt gcccatcctg gtcgagctgg
      721 acggcgacgt aaacggccac aagttcagcg tgcgcggcga gggcgagggc gatgccacca
      781 acggcaagct gaccctgaag ttcatctgca ccaccggcaa gctgcccgtg ccctggccca
      841 ccctcgtgac caccctgacc tacggcgtgc agtgcttcag ccgctacccc gaccacatga
      901 agcgccacga cttcttcaag tccgccatgc ccgaaggcta cgtccaggag cgcaccatca
      961 gcttcaagga cgacggcacc tacaagaccc gcgccgaggt gaagttcgag ggcgacaccc
     1021 tggtgaaccg catcgagctg aagggcatcg acttcaagg
//
