LOCUS       sequence-127533-        1031 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses rat Cx26 (Gjb2 CDS) with a 7 amino acid linker on the 
            C-terminus of the Connexin linking to non-monomerized superfolder 
            GFP. Expression driven by CMV promoter..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1031)
  AUTHORS   Stout RF Jr, Snapp EL, Spray DC
  TITLE     Connexin Type and Fluorescent Protein-fusion Tag Determine 
            Structural Stability of Gap Junction Plaques.
  JOURNAL   J Biol Chem. 2015 Aug 11. pii: jbc.M115.659979.
  PUBMED    26265468
REFERENCE   2  (bases 1 to 1031)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Biol 
            Chem. 2015 Aug 11. pii: jbc.M115.659979."
COMMENT     Sequence Label: Cx26-sfGFP
FEATURES             Location/Qualifiers
     source          1..1031
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(join(3..713,714..716,717..719))
                     /codon_start=1
                     /product="GFP variant that folds robustly even when fused 
                     to poorly folded proteins (Pedelacq et al., 2006)"
                     /label=superfolder GFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVRGEGEGDATNGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKRHDFFKSAMPEGYVQERTISFKDD
                     GTYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNFNSHNVYITADKQKNGIK
                     ANFKIRHNVEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSVLSKDPNEKRDHMVLL
                     EFVTAAGITHGMDELYK"
     primer_bind     complement(45..66)
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     653..674
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     regulatory      716..725
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 tacttgtaca gctcgtccat gccgtgagtg atcccggcgg cggtcacgaa ctccagcagg
       61 accatgtgat cgcgcttctc gttggggtct ttgctcagca cggactgggt gctcaggtag
      121 tggttgtcgg gcagcagcac ggggccgtcg ccgatggggg tgttctgctg gtagtggtcg
      181 gcgagctgca cgctgccgtc ctccacgttg tggcggatct tgaagttggc cttgatgccg
      241 ttcttctgct tgtcggcggt gatatagacg ttgtggctgt tgaagttgta ctccagcttg
      301 tgccccagga tgttgccgtc ctccttgaag tcgatgccct tcagctcgat gcggttcacc
      361 agggtgtcgc cctcgaactt cacctcggcg cgggtcttgt aggtgccgtc gtccttgaag
      421 ctgatggtgc gctcctggac gtagccttcg ggcatggcgg acttgaagaa gtcgtggcgc
      481 ttcatgtggt cggggtagcg gctgaagcac tgcacgccgt aggtcagggt ggtcacgagg
      541 gtgggccagg gcacgggcag cttgccggtg gtgcagatga acttcagggt cagcttgccg
      601 ttggtggcat cgccctcgcc ctcgccgcgc acgctgaact tgtggccgtt tacgtcgccg
      661 tccagctcga ccaggatggg caccaccccg gtgaacagct cctcgccctt gctcaccatg
      721 gtggcgaccg gtggatccgc gactggtctt ttggacttcc ctgagcaata cctaatgaac
      781 agatagcaca gctctgtgat gtttagcagg atgcaaattc cagacacaga gatcatgaac
      841 accgtgaaga cagtcttttc tgtgggcctg gaaatgaagc agtccactgt attgggacaa
      901 ggccaggcgt tacacttcac cagacgctgc atgaagaagc cattgtacat gatgtaaaag
      961 acatacatga agacagcttc gaagatgacc cggaagaaga tgctggtggt gtaggtccac
     1021 cacagggacc c
//
