LOCUS       sequence-127536-        1120 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Gateway destination vector for cell-free expression with N-term 
            myc-tag mCherry tag.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1120)
  AUTHORS   Gagoski D, Mureev S, Giles N, Johnston W, Dahmer-Heath M, Skalamera 
            D, Gonda TJ, Alexandrov K
  TITLE     Gateway-compatible vectors for high-throughput protein expression in
            pro- and eukaryotic cell-free systems.
  JOURNAL   J Biotechnol. 2015 Feb 10;195:1-7. doi: 
            10.1016/j.jbiotec.2014.12.006. Epub 2014 Dec 19.
  PUBMED    25529348
REFERENCE   2  (bases 1 to 1120)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J 
            Biotechnol."; date: "2015-02-10"; volume: "195:1-7. doi"; pages: " 
            10.1016/j.jbiotec.2014.12.006. Epub 2014 Dec 19"
COMMENT     Sequence Label: pCellFree_G07
FEATURES             Location/Qualifiers
     source          1..1120
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             109..138
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             148..171
                     /codon_start=1
                     /product="8xHis affinity tag"
                     /label=8xHis
                     /translation="HHHHHHHH"
     CDS             178..879
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="SKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEGTQ
                     TAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNFED
                     GGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALKGE
                     IKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERAEG
                     RHSTGGMDELYK"
     primer_bind     complement(317..335)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     585..604
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     CDS             880..903
                     /codon_start=1
                     /product="recognition and cleavage site for human 
                     rhinovirus 3C and PreScission proteases"
                     /label=HRV 3C site
                     /translation="LEVLFQGP"
     protein_bind    904..1028
                     /gene="mutant version of attR"
                     /label=attR1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
ORIGIN
        1 tttattttat tttattttat tttattttat tttattttat tttatttaac catgacagta
       61 atgtataaag tctgtaaaga cattaaacac gtaagtgaaa ccatgggcga gcagaagctg
      121 atctcggagg aggatctgac ctctggtcac caccatcacc accatcacca cggcacaagc
      181 aagggcgagg aggataacat ggccatcatc aaggagttca tgcgcttcaa ggtgcacatg
      241 gagggctccg tgaacggcca cgagttcgag atcgagggcg agggcgaggg ccgcccctac
      301 gagggcaccc agaccgccaa gctgaaggtg accaagggtg gccccctgcc cttcgcctgg
      361 gacatcctgt cccctcagtt catgtacggc tccaaggcct acgtgaagca ccccgccgac
      421 atccccgact acttgaagct gtccttcccc gagggcttca agtgggagcg cgtgatgaac
      481 ttcgaggacg gcggcgtggt gaccgtgacc caggactcct ccctgcagga cggcgagttc
      541 atctacaagg tgaagctgcg cggcaccaac ttcccctccg acggccccgt aatgcagaag
      601 aagactatgg gctgggaggc ctcctccgag cggatgtacc ccgaggacgg cgccctgaag
      661 ggcgagatca agcagaggct gaagctgaag gacggcggcc actacgacgc tgaggtcaag
      721 accacctaca aggccaagaa gcccgtgcag ctgcccggcg cctacaacgt caacatcaag
      781 ttggacatca cctcccacaa cgaggactac accatcgtgg aacagtacga acgcgccgag
      841 ggccgccact ccaccggcgg catggacgag ctgtacaagc tggaggtgct cttccagggt
      901 ccgacaagtt tgtacaaaaa agctgaacga gaaacgtaaa atgatataaa tatcaatata
      961 ttaaattaga ttttgcataa aaaacagact acataatact gtaaaacaca acatatccag
     1021 tcactatggg gcccgtcgac ctgcagactg gctgtgtata agggagcctg acatttatat
     1081 tccccagaac atcaggttaa tggcgttttt gatgtcattt
//
