LOCUS       sequence-127537-         971 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Gateway destination vector for cell-free expression with N-term 
            myc-tag mCherry tag.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 971)
  AUTHORS   Gagoski D, Mureev S, Giles N, Johnston W, Dahmer-Heath M, Skalamera 
            D, Gonda TJ, Alexandrov K
  TITLE     Gateway-compatible vectors for high-throughput protein expression in
            pro- and eukaryotic cell-free systems.
  JOURNAL   J Biotechnol. 2015 Feb 10;195:1-7. doi: 
            10.1016/j.jbiotec.2014.12.006. Epub 2014 Dec 19.
  PUBMED    25529348
REFERENCE   2  (bases 1 to 971)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J 
            Biotechnol."; date: "2015-02-10"; volume: "195:1-7. doi"; pages: " 
            10.1016/j.jbiotec.2014.12.006. Epub 2014 Dec 19"
COMMENT     Sequence Label: pCellFree_G07
FEATURES             Location/Qualifiers
     source          1..971
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        344..446
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding 
                     chloramphenicol acetyltransferase"
     primer_bind     complement(513..532)
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
ORIGIN
        1 cttccttcct gcagggtacc acgcgtaccc aacacacgga cacatgcacg aacacgcatg
       61 cagagagatc atacatcatc tatagagaag tacacgtaaa agtaagactc gtcgtctgcc
      121 agctacaacc gagaaggcga cgtgaaagga acaagaaagg aggaggaggg cggccgctta
      181 caccactttg tacaagaaag cttgctaagt tggcagcatc acccgacgca ctttgcgccg
      241 aataaatacc tgtgacggaa gatcacttcg cagaataaat aaatcctggt gtccctgttg
      301 ataccgggaa gccctgggcc aacttttggc gaaaatgaga cgttgatcgg cacgcaagag
      361 gttccaactt tcaccataat gaaataagat cactaccggg cgtatttttt gagttatcga
      421 gattttcagg agctaaggaa gctaaaatgg agaaaaaaat cactggatat accaccgttg
      481 atatatccca atggcatcgt aaagaacatt ttgaggcatt tcagtcagtt gctcaatgta
      541 cctataacca gaccgttcag ctggatatta cggccttttt aaagaccgta aagaaaaata
      601 agcacaagtt ttatccggcc tttattcaca ttcttgcccg cctgatgaat gctcatccgg
      661 aattccgtat ggcaatgaaa gacggtgagc tggtgatatg ggatagtgtt cacccttgtt
      721 acaccgtttt ccatgagcaa actgaaacgt tttcatcgct ctggagtgaa taccacgacg
      781 atttccggca gtttctacac atatattcgc aagatgtggc gtgttacggt gaaaacctgg
      841 cctatttccc taaagggttt attgagaata tgtttttcgt ctcagccaat ccctgggtga
      901 gtttcaccag ttttgattta aacgtggcca atatggacaa cttcttcgcc cccgttttca
      961 ccatgggcaa a
//
