LOCUS       sequence-127538-        1040 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Gateway destination vector for cell-free expression with N-term 
            myc-tag mCherry tag.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1040)
  AUTHORS   Gagoski D, Mureev S, Giles N, Johnston W, Dahmer-Heath M, Skalamera 
            D, Gonda TJ, Alexandrov K
  TITLE     Gateway-compatible vectors for high-throughput protein expression in
            pro- and eukaryotic cell-free systems.
  JOURNAL   J Biotechnol. 2015 Feb 10;195:1-7. doi: 
            10.1016/j.jbiotec.2014.12.006. Epub 2014 Dec 19.
  PUBMED    25529348
REFERENCE   2  (bases 1 to 1040)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J 
            Biotechnol."; date: "2015-02-10"; volume: "195:1-7. doi"; pages: " 
            10.1016/j.jbiotec.2014.12.006. Epub 2014 Dec 19"
COMMENT     Sequence Label: pCellFree_G07
FEATURES             Location/Qualifiers
     source          1..1040
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             253..276
                     /codon_start=1
                     /product="recognition and cleavage site for human 
                     rhinovirus 3C and PreScission proteases"
                     /label=HRV 3C site
                     /translation="LEVLFQGP"
     protein_bind    277..401
                     /gene="mutant version of attR"
                     /label=attR1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     CDS             complement(448..753)
                     /codon_start=1
                     /gene="ccdB"
                     /product="CcdB, a bacterial toxin that poisons DNA gyrase"
                     /label=ccdB
                     /note="Plasmids containing the ccdB gene cannot be 
                     propagated in standard E. coli strains."
                     /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK
                     VSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     primer_bind     complement(550..569)
                     /label=ccdB-fwd
                     /note="ccdB gene, forward primer"
ORIGIN
        1 gagcggatgt accccgagga cggcgccctg aagggcgaga tcaagcagag gctgaagctg
       61 aaggacggcg gccactacga cgctgaggtc aagaccacct acaaggccaa gaagcccgtg
      121 cagctgcccg gcgcctacaa cgtcaacatc aagttggaca tcacctccca caacgaggac
      181 tacaccatcg tggaacagta cgaacgcgcc gagggccgcc actccaccgg cggcatggac
      241 gagctgtaca agctggaggt gctcttccag ggtccgacaa gtttgtacaa aaaagctgaa
      301 cgagaaacgt aaaatgatat aaatatcaat atattaaatt agattttgca taaaaaacag
      361 actacataat actgtaaaac acaacatatc cagtcactat ggggcccgtc gacctgcaga
      421 ctggctgtgt ataagggagc ctgacattta tattccccag aacatcaggt taatggcgtt
      481 tttgatgtca ttttcgcggt ggctgagatc agccacttct tccccgataa cggagaccgg
      541 cacactggcc atatcggtgg tcatcatgcg ccagctttca tccccgatat gcaccaccgg
      601 gtaaagttca cgggagactt tatctgacag cagacgtgca ctggccaggg ggatcaccat
      661 ccgtcgcccg ggcgtgtcaa taatatcact ctgtacatcc acaaacagac gataacggct
      721 ctctctttta taggtgtaaa ccttaaactg catttcacca gcccctgttc tcgtcagcaa
      781 aagagccgtt catttcaata aaccgggcga cctcagccat cccttcctga ttttccgctt
      841 tccagcgttc ggcacgcaga cgacgggctt cattctgcat ggttgtgctt accagaccgg
      901 agatattgac atcatatatg ccttgagcaa ctgatagctg tcgctgtcaa ctgtcactgt
      961 aatacgctgc ttcatagcat acctcttttt gacatacttc gggtatacat atcagtatat
     1021 attcttatac cgcaaaaatc
//
