LOCUS       sequence-127542-        1075 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Standard for cell-free expression benchmarking..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1075)
  AUTHORS   Gagoski D, Polinkovsky ME, Mureev S, Kunert A, Johnston W, Gambin Y,
            Alexandrov K
  TITLE     Performance benchmarking of four cell-free protein expression 
            systems.
  JOURNAL   Biotechnol Bioeng. 2015 Aug 24. doi: 10.1002/bit.25814.
  PUBMED    26301602
REFERENCE   2  (bases 1 to 1075)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Biotechnol 
            Bioeng. 2015 Aug 24. doi: 10.1002/bit.25814."
COMMENT     Sequence Label: pCellFree_G03 NFKB1
FEATURES             Location/Qualifiers
     source          1..1075
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    182..206
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
ORIGIN
        1 cttccttcct gcagggtacc acgcgtaccc aacacacgga cacatgcacg aacacgcatg
       61 cagagagatc atacatcatc tatagagaag tacacgtaaa agtaagactc gtcgtctgcc
      121 agctacaacc gagaaggcga cgtgaaagga acaagaaagg aggaggaggg cggccgctta
      181 caccactttg tacaagaaag ttgggtaaat tttgccttct agaggtcctt cctgcccata
      241 atcatggggc attttgttga gagttagcag tgaggcacca ctggtcagag actcggtaaa
      301 gctgagtttg cggaaggatg tctccacgcc gctgtcgcag acactgtcac tgtctcggag
      361 ctcgtctatt tgctgccttg tggaggcagg cgagagaggc agcgagtggg cctgagaggt
      421 ggtcttcact gggctggagg ctgcctggat cacttcaatt gcttcggtgt agcccatttg
      481 tctcagggcc tccaccagct ctctgactgt acccccagag acctcatagt tgtccataag
      541 tgttttggaa ggagcaggac tcagccggaa ggcattatta agtatcccca gacctaattt
      601 ctgcgccaga gtagcccagt ttttgtctgg atcaggaatt tctagtaact tatacagctg
      661 cagcttcaca tcttcagcca gctgtttcat gtctccttgt gctagtaaat catcagatgt
      721 aaactctggc tcatatggtt tcccatttaa tatgtcaaat acctgccagc tggtggccat
      781 atctagaggc gtggttccag gcacaactcc ttcatcctct cctgcatttt cccaagagtc
      841 atccaggtca tagagaggct caaagttctc caccagggga tctgctcctg ctgctttgag
      901 aagagctgcc agcctggtgg accctctccc agctgctata tgcaggggtg tggttccatc
      961 gtaggtagta ctgtccacat gggcatcacc ctccaggagc aggcagcctg ccaatgagat
     1021 gttgtcgtgc tccacagcca ggtgcagtgc tgtgcgcccg gacttctgct cctga
//
